cDNA Clone Cyclin K/CDK12

• Synonyms: CRK7; CRKR; CRKRS; hCDK12
• Plasmid: pFBDM Cyclin K/6His-CDK12
• Parent Plasmid: pFBDM
• Expressed: 6His-CDK12
• Genbank: NM_016507.2 GI:157817022
• Tag: 6His
• Species: Human

Sequence of Insert

GCTAGCGAAACCATGCACCATCACCATCACCATATGCCCAATTCAGAGAGACATGGGGGCAAGAAGGACGGGAGTGGAGGAGCTTCTGGAACTTTGCAGCCGTCATCGGGAGGCGGCAGCTCTAACAGCAGAGAGCGTCACCGCTTGGTATCGAAGCACAAGCGGCATAAGTCCAAACACTCCAAAGACATGGGGTTGGTGACCCCCGAAGCAGCATCCCTGGGCACAGTTATCAAACCTTTGGTGGAGTATGATGATATCAGCTCTGATTCCGACACCTTCTCCGATGACATGGCCTTCAAACTAGACCGAAGGGAGAACGACGAACGTCGTGGATCAGATCGGAGCGACCGCCTGCACAAACATCGTCACCACCAGCACAGGCGTTCCCGGGACTTACTAAAAGCTAAACAGACCGAAAAAGAAAAAAGCCAAGAAGTCTCCAGCAAGTCGGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGGAGACTGATGACTATGGGAAGGCGCAGGTAGCCAAAAGCAGCAGCAAGGAATCCAGGTCATCCAAGCTCCACAAGGAGAAGACCAGGAAAGAACGGGAGCTGAAGTCTGGGCACAAAGACCGGAGTAAAAGTCATCGAAAAAGGGAAACACCCAAAAGTTACAAAACAGTGGACAGCCCAAAACGGAGATCCAGGAGCCCCCACAGGAAGTGGTCTGACAGCTCCAAACAAGATGATAGCCCCTCGGGAGCTTCTTATGGCCAAGATTATGACCTTAGTCCCTCACGATCTCATACCTCGAGCAATTATGACTCCTACAAGAAAAGTCCTGGAAGTACCTCGAGAAGGCAGTCGGTCAGTCCCCCTTACAAGGAGCCTTCGGCCTACCAGTCCAGCACCCGGTCACCGAGCCCCTACAGTAGGCGACAGAGATCTGTCAGTCCCTATAGCAGGAGACGGTCGTCCAGCTACGAAAGAAGTGGCTCTTACAGCGGGCGATCGCCCAGTCCCTATGGTCGAAGGCGGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTCCCCAGTAGGAAATCCATGAAGTCCAGAAGTAGAAGTCCTGCATATTCAAGACATTCATCTTCTCATAGTAAAAAGAAGAGATCCAGTTCACGCAGTCGTCATTCCAGTATCTCACCTGTCAGGCTTCCACTTAATTCCAGTCTGGGAGCTGAACTCAGTAGGAAAAAGAAGGAAAGAGCAGCTGCTGCTGCTGCAGCAAAGATGGATGGAAAGGAGTCCAAGGGTTCACCTGTATTTTTGCCTAGAAAAGAGAACAGTTCAGTAGAGGCTAAGGATTCAGGTTTGGAGTCTAAAAAGTTACCCAGAAGTGTAAAATTGGAAAAATCTGCCCCAGATACTGAACTGGTGAATGTAACACATCTAAACACAGAGGTAAAAAATTCTTCAGATACAGGGAAAGTAAAGTTGGATGAGAACTCCGAGAAGCATCTTGTTAAAGATTTGAAAGCACAGGGAACAAGAGACTCTAAACCCATAGCACTGAAAGAGGAGATTGTTACTCCAAAGGAGACAGAAACATCAGAAAAGGAGACCCCTCCACCTCTTCCCACAATTGCTTCTCCCCCACCCCCTCTACCAACTACTACCCCTCCACCTCAGACACCCCCTTTGCCACCTTTGCCTCCAATACCAGCTCTTCCACAGCAACCACCTCTGCCTCCTTCTCAGCCAGCATTTAGTCAGGTTCCTGCTTCCAGTACTTCAACTTTGCCCCCTTCTACTCACTCAAAGACATCTGCTGTGTCCTCTCAGGCAAATTCTCAGCCCCCTGTACAGGTTTCTGTGAAGACTCAAGTATCTGTAACAGCTGCTATTCCACACCTGAAAACTTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATGACATGGATAGTCCAAAAGAAACTCTTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTACTCACAGACCTTCCTCTCCCTCCAGAGCTCCCTGGTGGAGATCTGTCTCCCCCAGACTCTCCAGAACCAAAGGCAATCACACCACCTCAGCAACCATATAAAAAGAGACCAAAAATTTGTTGTCCTCGTTATGGAGAAAGAAGACAAACAGAAAGCGACTGGGGGAAACGCTGTGTGGACAAGTTTGACATTATTGGGATTATTGGAGAAGGAACCTATGGCCAAGTATATAAAGCCAAGGACAAAGACACAGGAGAACTAGTGGCTCTGAAGAAGGTGAGACTAGACAATGAGAAAGAGGGCTTCCCAATCACAGCCATTCGTGAAATCAAAATCCTTCGTCAGTTAATCCACCGAAGTGTTGTTAACATGAAGGAAATTGTCACAGATAAACAAGATGCACTGGATTTCAAGAAGGACAAAGGTGCCTTTTACCTTGTATTTGAGTATATGGACCATGACTTAATGGGACTGCTAGAATCTGGTTTGGTGCACTTTTCTGAGGACCATATCAAGTCGTTCATGAAACAGCTAATGGAAGGATTGGAATACTGTCACAAAAAGAATTTCCTGCATCGGGATATTAAGTGTTCTAACATTTTGCTGAATAACAGTGGGCAAATCAAACTAGCAGATTTTGGACTTGCTCGGCTCTATAACTCTGAAGAGAGTCGCCCTTACACAAACAAAGTCATTACTTTGTGGTACCGACCTCCAGAACTACTGCTAGGAGAGGAACGTTACACACCAGCCATAGATGTTTGGAGCTGTGGATGTATTCTTGGGGAACTATTCACAAAGAAGCCTATTTTTCAAGCCAATCTGGAACTGGCTCAGCTAGAACTGATCAGCCGACTTTGTGGTAGCCCTTGTCCAGCTGTGTGGCCTGATGTTATCAAACTGCCCTACTTCAACACCATGAAACCGAAGAAGCAATATCGAAGGCGTCTACGAGAAGAATTCTCTTTCATTCCTTCTGCAGCACTTGATTTATTGGACCACATGCTGACACTAGATCCTAGTAAGCGGTGCACAGCTGAACAGACCCTACAGAGCGACTTCCTTAAAGATGTCGAACTCAGCAAAATGGCTCCTCCAGACCTCCCCCACTGGCAGGATTGCCATGAGTTGTGGAGTAAGAAACGGCGACGTCAGCGACAAAGTGGTGTTGTAGTCGAAGAGCCACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTGAGCCTGTGAAGAACAGCAGCCCAGCACCACCTCAGCCTGCTCCTGGCAAGGTGGAGTCTGGGGCTGGGGATGCAATAGGCCTTGCTGACATCACACAACAGCTGAATCAAAGTGAATTGGCAGTGTTATTAAACCTGCTGCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACATCCACTCCAACCCAGAGATGCAGCAGCAGCTGGAAGCCCTGAACCAATCCATCAGTGCCCTGACGGAAGCTACTTCCCAGCAGCAGGACTCAGAGACCATGGCCCCAGAGGAGTCTTTGAAGGAAGCACCCTCTGCCCCAGTGATCCTGCCTTCAGCAGAACAGACGACCCTTGAAGCTTCAAGCACACCAGCTGACATGCAGAATATATTGGCAGTTCTCTTGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGGCAGTCTGGAGGAAAACAACAGTGACAAGAACAGTGGGCCACAGGGGCCCCGAAGAACTCCCACAATGCCACAGGAGGAGGCAGCAGCATGTCCTCCTCACATTCTTCCACCAGAGAAGAGGCCCCCTGAGCCCCCCGGACCTCCACCGCCGCCACCTCCACCCCCTCTGGTTGAAGGCGATCTTTCCAGCGCCCCCCAGGAGTTGAACCCAGCCGTGACAGCCGCCTTGCTGCAACTTTTATCCCAGCCTGAAGCAGAGCCTCCTGGCCACCTGCCACATGAGCACCAGGCCTTGAGACCAATGGAGTACTCCACCCGACCCCGTCCAAACAGGACTTATGGAAACACTGATGGGCCTGAAACAGGGTTCAGTGCCATTGACACTGATGAACGAAACTCTGGTCCAGCCTTGACAGAATCCTTGGTCCAGACCCTGGTGAAGAACAGGACCTTCTCAGGCTCTCTGAGCCACCTTGGGGAGTCCAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACCTCCGTTTTGCCAGGGTCCCCTTAGCGTTACACCCGGTGGTCGGGCAACCATTCCTGAAGGCTGAGGGAAGCAGCAATTCTGTGGTACATGCAGAGACCAAATTGCAAAACTATGGGGAGCTGGGGCCAGGAACCACTGGGGCCAGCAGCTCAGGAGCAGGCCTTCACTGGGGGGGCCCAACTCAGTCTTCTGCTTATGGAAAACTCTATCGGGGGCCTACAAGAGTCCCACCAAGAGGGGGAAGAGGGAGAGGAGTTCCTTACTAAATGCAT

Amino Acid Sequence

MHHHHHHMPNSERHGGKKDGSGGASGTLQPSSGGGSSNSRERHRLVSKHK
RHKSKHSKDMGLVTPEAASLGTVIKPLVEYDDISSDSDTFSDDMAFKLDR
RENDERRGSDRSDRLHKHRHHQHRRSRDLLKAKQTEKEKSQEVSSKSGSM
KDRISGSSKRSNEETDDYGKAQVAKSSSKESRSSKLHKEKTRKERELKSG
HKDRSKSHRKRETPKSYKTVDSPKRRSRSPHRKWSDSSKQDDSPSGASYG
QDYDLSPSRSHTSSNYDSYKKSPGSTSRRQSVSPPYKEPSAYQSSTRSPS
PYSRRQRSVSPYSRRRSSSYERSGSYSGRSPSPYGRRRSSSPFLSKRSLS
RSPLPSRKSMKSRSRSPAYSRHSSSHSKKKRSSSRSRHSSISPVRLPLNS
SLGAELSRKKKERAAAAAAAKMDGKESKGSPVFLPRKENSSVEAKDSGLE
SKKLPRSVKLEKSAPDTELVNVTHLNTEVKNSSDTGKVKLDENSEKHLVK
DLKAQGTRDSKPIALKEEIVTPKETETSEKETPPPLPTIASPPPPLPTTT
PPPQTPPLPPLPPIPALPQQPPLPPSQPAFSQVPASSTSTLPPSTHSKTS
AVSSQANSQPPVQVSVKTQVSVTAAIPHLKTSTLPPLPLPPLLPGDDDMD
SPKETLPSKPVKKEKEQRTRHLLTDLPLPPELPGGDLSPPDSPEPKAITP
PQQPYKKRPKICCPRYGERRQTESDWGKRCVDKFDIIGIIGEGTYGQVYK
AKDKDTGELVALKKVRLDNEKEGFPITAIREIKILRQLIHRSVVNMKEIV
TDKQDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFMKQL
MEGLEYCHKKNFLHRDIKCSNILLNNSGQIKLADFGLARLYNSEESRPYT
NKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQANLELA
QLELISRLCGSPCPAVWPDVIKLPYFNTMKPKKQYRRRLREEFSFIPSAA
LDLLDHMLTLDPSKRCTAEQTLQSDFLKDVELSKMAPPDLPHWQDCHELW
SKKRRRQRQSGVVVEEPPPSKTSRKETTSGTSTEPVKNSSPAPPQPAPGK
VESGAGDAIGLADITQQLNQSELAVLLNLLQSQTDLSIPQMAQLLNIHSN
PEMQQQLEALNQSISALTEATSQQQDSETMAPEESLKEAPSAPVILPSAE
QTTLEASSTPADMQNILAVLLSQLMKTQEPAGSLEENNSDKNSGPQGPRR
TPTMPQEEAAACPPHILPPEKRPPEPPGPPPPPPPPPLVEGDLSSAPQEL
NPAVTAALLQLLSQPEAEPPGHLPHEHQALRPMEYSTRPRPNRTYGNTDG
PETGFSAIDTDERNSGPALTESLVQTLVKNRTFSGSLSHLGESSSYQGTG
SVQFPGDQDLRFARVPLALHPVVGQPFLKAEGSSNSVVHAETKLQNYGEL
GPGTTGASSSGAGLHWGGPTQSSAYGKLYRGPTRVPPRGGRGRGVPY*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU45784
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 6His-CDK12 cloned from DU45683 into DU45750 at Nhe1/NsiI