cDNA Clone Cyclin K/CDK12

• Synonyms: CPR4; CCNK
• Plasmid: pFBDM Cyclin K/GST-CDK12
• Parent Plasmid: pFBDM
• Expressed: Cyclin K (untagged)
• Genbank: NM_001099402.1 GI:150417988
• Tag: untagged
• Species: Human

Sequence of Insert

AGATCTATGAAGGAGAATAAAGAAAATTCAAGCCCTTCAGTAACTTCAGCAAACCTGGACCACACAAAGCCATGTTGGTACTGGGATAAGAAAGACTTGGCTCATACACCCTCACAACTTGAAGGACTTGATCCAGCCACCGAGGCCCGGTACCGCCGAGAGGGCGCTCGGTTCATCTTTGATGTGGGCACACGTTTGGGGCTACACTATGATACCCTGGCAACTGGAATAATTTATTTTCATCGCTTCTATATGTTTCATTCCTTCAAGCAATTCCCAAGATATGTGACAGGAGCCTGTTGCCTCTTTCTGGCTGGGAAAGTAGAAGAAACACCAAAAAAATGTAAAGATATCATCAAAACAGCTCGTAGTTTATTAAATGATGTACAATTTGGCCAGTTTGGAGATGACCCAAAGGAGGAAGTAATGGTTCTGGAGAGAATCTTACTGCAGACCATCAAGTTTGATTTACAGGTAGAACATCCATACCAGTTCCTACTAAAATATGCAAAGCAACTCAAAGGTGATAAAAACAAAATTCAAAAGTTGGTTCAAATGGCATGGACATTTGTAAATGACAGTCTCTGCACCACCTTGTCACTGCAGTGGGAACCAGAGATCATAGCAGTAGCAGTGATGTATCTCGCAGGACGTTTGTGCAAATTTGAAATACAAGAATGGACCTCCAAACCCATGTATAGGAGATGGTGGGAGCAGTTTGTTCAAGATGTCCCGGTCGACGTTTTGGAAGACATCTGCCACCAAATCCTGGATCTTTACTCACAAGGAAAACAACAGATGCCTCATCACACCCCCCATCAGCTGCAACAGCCCCCATCTCTTCAGCCTACACCACAAGTGCCGCAAGTACAGCAGTCACAGCCGTCTCAAAGCTCCGAACCATCCCAGCCCCAGCAGAAGGACCCCCAGCAACCAGCCCAGCAGCAGCAGCCAGCCCAACAGCCCAAGAAACCCTCTCCGCAGCCCAGTTCTCCCCGACAGGTTAAGCGAGCCGTGGTTGTTTCTCCCAAAGAAGAGAACAAAGCAGCAGAACCACCACCACCTAAAATCCCCAAAATTGAGACCACTCATCCACCGTTGCCTCCAGCCCACCCACCTCCAGACCGGAAGCCTCCCCTCGCTGCTGCCTTAGGTGAGGCTGAGCCGCCGGGCCCTGTGGATGCCACTGACCTCCCCAAAGTCCAGATTCCCCCTCCGGCCCACCCGGCCCCTGTGCACCAGCCACCGCCGCTGCCACACCGGCCCCCGCCCCCACCCCCCTCCAGCTACATGACCGGGATGTCCACCACCAGCTCCTACATGTCTGGAGAGGGCTACCAGAGCCTGCAGTCCATGATGAAGACCGAGGGACCCTCCTACGGTGCCCTGCCCCCCGCCTACGGCCCACCTGCACACCTGCCCTACCACCCCCATGTCTACCCGCCCAACCCGCCCCCGCCACCTGTGCCTCCTCCCCCAGCCTCCTTCCCCCCACCTGCCATCCCACCCCCTACTCCTGGCTACCCCCCACCCCCACCCACCTACAACCCCAACTTCCCACCCCCACCCCCACGCCTCCCGCCTACCCACGCAGTCCCCCCTCATCCTCCTCCAGGGTTGGGCCTGCCGCCAGCCAGCTACCCACCTCCTGCCGTCCCCCCTGGAGGACAGCCTCCTGTGCCCCCGCCCATTCCCCCACCCGGCATGCCTCCAGTTGGGGGGCTGGGGCGGGCAGCCTGGATGAGATAAGCGGCCGC

Amino Acid Sequence

MKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTPSQLEGLDPATEARYR
REGARFIFDVGTRLGLHYDTLATGIIYFHRFYMFHSFKQFPRYVTGACCL
FLAGKVEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEVMVLERILLQT
IKFDLQVEHPYQFLLKYAKQLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQ
WEPEIIAVAVMYLAGRLCKFEIQEWTSKPMYRRWWEQFVQDVPVDVLEDI
CHQILDLYSQGKQQMPHHTPHQLQQPPSLQPTPQVPQVQQSQPSQSSEPS
QPQQKDPQQPAQQQQPAQQPKKPSPQPSSPRQVKRAVVVSPKEENKAAEP
PPPKIPKIETTHPPLPPAHPPPDRKPPLAAALGEAEPPGPVDATDLPKVQ
IPPPAHPAPVHQPPPLPHRPPPPPPSSYMTGMSTTSSYMSGEGYQSLQSM
MKTEGPSYGALPPAYGPPAHLPYHPHVYPPNPPPPPVPPPPASFPPPAIP
PPTPGYPPPPPTYNPNFPPPPPRLPPTHAVPPHPPPGLGLPPASYPPPAV
PPGGQPPVPPPIPPPGMPPVGGLGRAAWMR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU45797
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GST-CDK12 re-amplified and cut NheI-NsiI, cloned into DU45750 NheI-NsiI