cDNA Clone Cyclin K/CDK12

• Synonyms: CRK7; CRKR; CRKRS; hCDK12
• Plasmid: pFBDM Cyclin K/GST-CDK12
• Parent Plasmid: pFBDM
• Expressed: GST-CDK12
• Genbank: NM_016507.2 GI:157817022
• Tag: GST
• Species: Human

Sequence of Insert

gctagcgaaaccATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCT
TGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAG
AGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTT
GAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGT
TAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACA
ACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAA
GGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAA
AGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGC
TGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGAT
CATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTT
ATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTA
AAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGC
AAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGG
CGACCATCCTCCAAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGG
GATCCATGCCCAATTCAGAGAGACATGGGGGCAAGAAGGACGGGAGTGGA
GGAGCTTCTGGAACTTTGCAGCCGTCATCGGGAGGCGGCAGCTCTAACAG
CAGAGAGCGTCACCGCTTGGTATCGAAGCACAAGCGGCATAAGTCCAAAC
ACTCCAAAGACATGGGGTTGGTGACCCCCGAAGCAGCATCCCTGGGCACA
GTTATCAAACCTTTGGTGGAGTATGATGATATCAGCTCTGATTCCGACAC
CTTCTCCGATGACATGGCCTTCAAACTAGACCGAAGGGAGAACGACGAAC
GTCGTGGATCAGATCGGAGCGACCGCCTGCACAAACATCGTCACCACCAG
CACAGGCGTTCCCGGGACTTACTAAAAGCTAAACAGACCGAAAAAGAAAA
AAGCCAAGAAGTCTCCAGCAAGTCGGGATCGATGAAGGACCGGATATCGG
GAAGTTCAAAGCGTTCGAATGAGGAGACTGATGACTATGGGAAGGCGCAG
GTAGCCAAAAGCAGCAGCAAGGAATCCAGGTCATCCAAGCTCCACAAGGA
GAAGACCAGGAAAGAACGGGAGCTGAAGTCTGGGCACAAAGACCGGAGTA
AAAGTCATCGAAAAAGGGAAACACCCAAAAGTTACAAAACAGTGGACAGC
CCAAAACGGAGATCCAGGAGCCCCCACAGGAAGTGGTCTGACAGCTCCAA
ACAAGATGATAGCCCCTCGGGAGCTTCTTATGGCCAAGATTATGACCTTA
GTCCCTCACGATCTCATACCTCGAGCAATTATGACTCCTACAAGAAAAGT
CCTGGAAGTACCTCGAGAAGGCAGTCGGTCAGTCCCCCTTACAAGGAGCC
TTCGGCCTACCAGTCCAGCACCCGGTCACCGAGCCCCTACAGTAGGCGAC
AGAGATCTGTCAGTCCCTATAGCAGGAGACGGTCGTCCAGCTACGAAAGA
AGTGGCTCTTACAGCGGGCGATCGCCCAGTCCCTATGGTCGAAGGCGGTC
CAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTCCCCA
GTAGGAAATCCATGAAGTCCAGAAGTAGAAGTCCTGCATATTCAAGACAT
TCATCTTCTCATAGTAAAAAGAAGAGATCCAGTTCACGCAGTCGTCATTC
CAGTATCTCACCTGTCAGGCTTCCACTTAATTCCAGTCTGGGAGCTGAAC
TCAGTAGGAAAAAGAAGGAAAGAGCAGCTGCTGCTGCTGCAGCAAAGATG
GATGGAAAGGAGTCCAAGGGTTCACCTGTATTTTTGCCTAGAAAAGAGAA
CAGTTCAGTAGAGGCTAAGGATTCAGGTTTGGAGTCTAAAAAGTTACCCA
GAAGTGTAAAATTGGAAAAATCTGCCCCAGATACTGAACTGGTGAATGTA
ACACATCTAAACACAGAGGTAAAAAATTCTTCAGATACAGGGAAAGTAAA
GTTGGATGAGAACTCCGAGAAGCATCTTGTTAAAGATTTGAAAGCACAGG
GAACAAGAGACTCTAAACCCATAGCACTGAAAGAGGAGATTGTTACTCCA
AAGGAGACAGAAACATCAGAAAAGGAGACCCCTCCACCTCTTCCCACAAT
TGCTTCTCCCCCACCCCCTCTACCAACTACTACCCCTCCACCTCAGACAC
CCCCTTTGCCACCTTTGCCTCCAATACCAGCTCTTCCACAGCAACCACCT
CTGCCTCCTTCTCAGCCAGCATTTAGTCAGGTTCCTGCTTCCAGTACTTC
AACTTTGCCCCCTTCTACTCACTCAAAGACATCTGCTGTGTCCTCTCAGG
CAAATTCTCAGCCCCCTGTACAGGTTTCTGTGAAGACTCAAGTATCTGTA
ACAGCTGCTATTCCACACCTGAAAACTTCAACGTTGCCTCCTTTGCCCCT
CCCACCCTTATTACCTGGAGATGATGACATGGATAGTCCAAAAGAAACTC
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA
CTCACAGACCTTCCTCTCCCTCCAGAGCTCCCTGGTGGAGATCTGTCTCC
CCCAGACTCTCCAGAACCAAAGGCAATCACACCACCTCAGCAACCATATA
AAAAGAGACCAAAAATTTGTTGTCCTCGTTATGGAGAAAGAAGACAAACA
GAAAGCGACTGGGGGAAACGCTGTGTGGACAAGTTTGACATTATTGGGAT
TATTGGAGAAGGAACCTATGGCCAAGTATATAAAGCCAAGGACAAAGACA
CAGGAGAACTAGTGGCTCTGAAGAAGGTGAGACTAGACAATGAGAAAGAG
GGCTTCCCAATCACAGCCATTCGTGAAATCAAAATCCTTCGTCAGTTAAT
CCACCGAAGTGTTGTTAACATGAAGGAAATTGTCACAGATAAACAAGATG
CACTGGATTTCAAGAAGGACAAAGGTGCCTTTTACCTTGTATTTGAGTAT
ATGGACCATGACTTAATGGGACTGCTAGAATCTGGGTTGGTGCACTTTTC
TGAGGACCATATCAAGTCGTTCATGAAACAGCTAATGGAAGGATTGGAAT
ACTGTCACAAAAAGAATTTCCTGCATCGGGATATTAAGTGTTCTAACATT
TTGCTGAATAACAGTGGGCAAATCAAACTAGCAGATTTTGGACTTGCTCG
GCTCTATAACTCTGAAGAGAGTCGCCCTTACACAAACAAAGTCATTACTT
TGTGGTACCGACCTCCAGAACTACTGCTAGGAGAGGAACGTTACACACCA
GCCATAGATGTTTGGAGCTGTGGATGTATTCTTGGGGAACTATTCACAAA
GAAGCCTATTTTTCAAGCCAATCTGGAACTGGCTCAGCTAGAACTGATCA
GCCGACTTTGTGGTAGCCCTTGTCCAGCTGTGTGGCCTGATGTTATCAAA
CTGCCCTACTTCAACACCATGAAACCGAAGAAGCAATATCGAAGGCGTCT
ACGAGAAGAATTCTCTTTCATTCCTTCTGCAGCACTTGATTTATTGGACC
ACATGCTGACACTAGATCCTAGTAAGCGGTGCACAGCTGAACAGACCCTA
CAGAGCGACTTCCTTAAAGATGTCGAACTCAGCAAAATGGCTCCTCCAGA
CCTCCCCCACTGGCAGGATTGCCATGAGTTGTGGAGTAAGAAACGGCGAC
GTCAGCGACAAAGTGGTGTTGTAGTCGAAGAGCCACCTCCATCCAAAACT
TCTCGAAAAGAAACTACCTCAGGGACAAGTACTGAGCCTGTGAAGAACAG
CAGCCCAGCACCACCTCAGCCTGCTCCTGGCAAGGTGGAGTCTGGGGCTG
GGGATGCAATAGGCCTTGCTGACATCACACAACAGCTGAATCAAAGTGAA
TTGGCAGTGTTATTAAACCTGCTGCAGAGCCAAACCGACCTGAGCATCCC
TCAAATGGCACAGCTGCTTAACATCCACTCCAACCCAGAGATGCAGCAGC
AGCTGGAAGCCCTGAACCAATCCATCAGTGCCCTGACGGAAGCTACTTCC
CAGCAGCAGGACTCAGAGACCATGGCCCCAGAGGAGTCTTTGAAGGAAGC
ACCCTCTGCCCCAGTGATCCTGCCTTCAGCAGAACAGACGACCCTTGAAG
CTTCAAGCACACCAGCTGACATGCAGAATATATTGGCAGTTCTCTTGAGT
CAGCTGATGAAAACCCAAGAGCCAGCAGGCAGTCTGGAGGAAAACAACAG
TGACAAGAACAGTGGGCCACAGGGGCCCCGAAGAACTCCCACAATGCCAC
AGGAGGAGGCAGCAGCATGTCCTCCTCACATTCTTCCACCAGAGAAGAGG
CCCCCTGAGCCCCCCGGACCTCCACCGCCGCCACCTCCACCCCCTCTGGT
TGAAGGCGATCTTTCCAGCGCCCCCCAGGAGTTGAACCCAGCCGTGACAG
CCGCCTTGCTGCAACTTTTATCCCAGCCTGAAGCAGAGCCTCCTGGCCAC
CTGCCACATGAGCACCAGGCCTTGAGACCAATGGAGTACTCCACCCGACC
CCGTCCAAACAGGACTTATGGAAACACTGATGGGCCTGAAACAGGGTTCA
GTGCCATTGACACTGATGAACGAAACTCTGGTCCAGCCTTGACAGAATCC
TTGGTCCAGACCCTGGTGAAGAACAGGACCTTCTCAGGCTCTCTGAGCCA
CCTTGGGGAGTCCAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAG
GGGACCAGGACCTCCGTTTTGCCAGGGTCCCCTTAGCGTTACACCCGGTG
GTCGGGCAACCATTCCTGAAGGCTGAGGGAAGCAGCAATTCTGTGGTACA
TGCAGAGACCAAATTGCAAAACTATGGGGAGCTGGGGCCAGGAACCACTG
GGGCCAGCAGCTCAGGAGCAGGCCTTCACTGGGGGGGCCCAACTCAGTCT
TCTGCTTATGGAAAACTCTATCGGGGGCCTACAAGAGTCCCACCAAGAGG
GGGAAGAGGGAGAGGAGTTCCTTACTAAatgcat

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPNSERHGGKKDGSGGASG
TLQPSSGGGSSNSRERHRLVSKHKRHKSKHSKDMGLVTPEAASLGTVIKP
LVEYDDISSDSDTFSDDMAFKLDRRENDERRGSDRSDRLHKHRHHQHRRS
RDLLKAKQTEKEKSQEVSSKSGSMKDRISGSSKRSNEETDDYGKAQVAKS
SSKESRSSKLHKEKTRKERELKSGHKDRSKSHRKRETPKSYKTVDSPKRR
SRSPHRKWSDSSKQDDSPSGASYGQDYDLSPSRSHTSSNYDSYKKSPGST
SRRQSVSPPYKEPSAYQSSTRSPSPYSRRQRSVSPYSRRRSSSYERSGSY
SGRSPSPYGRRRSSSPFLSKRSLSRSPLPSRKSMKSRSRSPAYSRHSSSH
SKKKRSSSRSRHSSISPVRLPLNSSLGAELSRKKKERAAAAAAAKMDGKE
SKGSPVFLPRKENSSVEAKDSGLESKKLPRSVKLEKSAPDTELVNVTHLN
TEVKNSSDTGKVKLDENSEKHLVKDLKAQGTRDSKPIALKEEIVTPKETE
TSEKETPPPLPTIASPPPPLPTTTPPPQTPPLPPLPPIPALPQQPPLPPS
QPAFSQVPASSTSTLPPSTHSKTSAVSSQANSQPPVQVSVKTQVSVTAAI
PHLKTSTLPPLPLPPLLPGDDDMDSPKETLPSKPVKKEKEQRTRHLLTDL
PLPPELPGGDLSPPDSPEPKAITPPQQPYKKRPKICCPRYGERRQTESDW
GKRCVDKFDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPI
TAIREIKILRQLIHRSVVNMKEIVTDKQDALDFKKDKGAFYLVFEYMDHD
LMGLLESGLVHFSEDHIKSFMKQLMEGLEYCHKKNFLHRDIKCSNILLNN
SGQIKLADFGLARLYNSEESRPYTNKVITLWYRPPELLLGEERYTPAIDV
WSCGCILGELFTKKPIFQANLELAQLELISRLCGSPCPAVWPDVIKLPYF
NTMKPKKQYRRRLREEFSFIPSAALDLLDHMLTLDPSKRCTAEQTLQSDF
LKDVELSKMAPPDLPHWQDCHELWSKKRRRQRQSGVVVEEPPPSKTSRKE
TTSGTSTEPVKNSSPAPPQPAPGKVESGAGDAIGLADITQQLNQSELAVL
LNLLQSQTDLSIPQMAQLLNIHSNPEMQQQLEALNQSISALTEATSQQQD
SETMAPEESLKEAPSAPVILPSAEQTTLEASSTPADMQNILAVLLSQLMK
TQEPAGSLEENNSDKNSGPQGPRRTPTMPQEEAAACPPHILPPEKRPPEP
PGPPPPPPPPPLVEGDLSSAPQELNPAVTAALLQLLSQPEAEPPGHLPHE
HQALRPMEYSTRPRPNRTYGNTDGPETGFSAIDTDERNSGPALTESLVQT
LVKNRTFSGSLSHLGESSSYQGTGSVQFPGDQDLRFARVPLALHPVVGQP
FLKAEGSSNSVVHAETKLQNYGELGPGTTGASSSGAGLHWGGPTQSSAYG
KLYRGPTRVPPRGGRGRGVPY*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU45797
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GST-CDK12 re-amplified and cut NheI-NsiI, cloned into DU45750 NheI-NsiI