cDNA Clone Cyclin K/CDK12

• Synonyms: CPR4; CCNK
• Plasmid: pFBDM GST-Cyclin K/CDK12 (untagged)
• Parent Plasmid: pFBDM
• Expressed: GST-Cyclin K
• Genbank: NM_001099402.1 GI:150417988
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGAAACCATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCTATGAAGGAGAATAAAGAAAATTCAAGCCCTTCAGTAACTTCAGCAAACCTGGACCACACAAAGCCATGTTGGTACTGGGATAAGAAAGACTTGGCTCATACACCCTCACAACTTGAAGGACTTGATCCAGCCACCGAGGCCCGGTACCGCCGAGAGGGCGCTCGGTTCATCTTTGATGTGGGCACACGTTTGGGGCTACACTATGATACCCTGGCAACTGGAATAATTTATTTTCATCGCTTCTATATGTTTCATTCCTTCAAGCAATTCCCAAGATATGTGACAGGAGCCTGTTGCCTCTTTCTGGCTGGGAAAGTAGAAGAAACACCAAAAAAATGTAAAGATATCATCAAAACAGCTCGTAGTTTATTAAATGATGTACAATTTGGCCAGTTTGGAGATGACCCAAAGGAGGAAGTAATGGTTCTGGAGAGAATCTTACTGCAGACCATCAAGTTTGATTTACAGGTAGAACATCCATACCAGTTCCTACTAAAATATGCAAAGCAACTCAAAGGTGATAAAAACAAAATTCAAAAGTTGGTTCAAATGGCATGGACATTTGTAAATGACAGTCTCTGCACCACCTTGTCACTGCAGTGGGAACCAGAGATCATAGCAGTAGCAGTGATGTATCTCGCAGGACGTTTGTGCAAATTTGAAATACAAGAATGGACCTCCAAACCCATGTATAGGAGATGGTGGGAGCAGTTTGTTCAAGATGTCCCGGTCGACGTTTTGGAAGACATCTGCCACCAAATCCTGGATCTTTACTCACAAGGAAAACAACAGATGCCTCATCACACCCCCCATCAGCTGCAACAGCCCCCATCTCTTCAGCCTACACCACAAGTGCCGCAAGTACAGCAGTCACAGCCGTCTCAAAGCTCCGAACCATCCCAGCCCCAGCAGAAGGACCCCCAGCAACCAGCCCAGCAGCAGCAGCCAGCCCAACAGCCCAAGAAACCCTCTCCGCAGCCCAGTTCTCCCCGACAGGTTAAGCGAGCCGTGGTTGTTTCTCCCAAAGAAGAGAACAAAGCAGCAGAACCACCACCACCTAAAATCCCCAAAATTGAGACCACTCATCCACCGTTGCCTCCAGCCCACCCACCTCCAGACCGGAAGCCTCCCCTCGCTGCTGCCTTAGGTGAGGCTGAGCCGCCGGGCCCTGTGGATGCCACTGACCTCCCCAAAGTCCAGATTCCCCCTCCGGCCCACCCGGCCCCTGTGCACCAGCCACCGCCGCTGCCACACCGGCCCCCGCCCCCACCCCCCTCCAGCTACATGACCGGGATGTCCACCACCAGCTCCTACATGTCTGGAGAGGGCTACCAGAGCCTGCAGTCCATGATGAAGACCGAGGGACCCTCCTACGGTGCCCTGCCCCCCGCCTACGGCCCACCTGCACACCTGCCCTACCACCCCCATGTCTACCCGCCCAACCCGCCCCCGCCACCTGTGCCTCCTCCCCCAGCCTCCTTCCCCCCACCTGCCATCCCACCCCCTACTCCTGGCTACCCCCCACCCCCACCCACCTACAACCCCAACTTCCCACCCCCACCCCCACGCCTCCCGCCTACCCACGCAGTCCCCCCTCATCCTCCTCCAGGGTTGGGCCTGCCGCCAGCCAGCTACCCACCTCCTGCCGTCCCCCCTGGAGGACAGCCTCCTGTGCCCCCGCCCATTCCCCCACCCGGCATGCCTCCAGTTGGGGGGCTGGGGCGGGCAGCCTGGATGAGATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMKENKENSSPSVTSANLDH
TKPCWYWDKKDLAHTPSQLEGLDPATEARYRREGARFIFDVGTRLGLHYD
TLATGIIYFHRFYMFHSFKQFPRYVTGACCLFLAGKVEETPKKCKDIIKT
ARSLLNDVQFGQFGDDPKEEVMVLERILLQTIKFDLQVEHPYQFLLKYAK
QLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQWEPEIIAVAVMYLAGRLCK
FEIQEWTSKPMYRRWWEQFVQDVPVDVLEDICHQILDLYSQGKQQMPHHT
PHQLQQPPSLQPTPQVPQVQQSQPSQSSEPSQPQQKDPQQPAQQQQPAQQ
PKKPSPQPSSPRQVKRAVVVSPKEENKAAEPPPPKIPKIETTHPPLPPAH
PPPDRKPPLAAALGEAEPPGPVDATDLPKVQIPPPAHPAPVHQPPPLPHR
PPPPPPSSYMTGMSTTSSYMSGEGYQSLQSMMKTEGPSYGALPPAYGPPA
HLPYHPHVYPPNPPPPPVPPPPASFPPPAIPPPTPGYPPPPPTYNPNFPP
PPPRLPPTHAVPPHPPPGLGLPPASYPPPAVPPGGQPPVPPPIPPPGMPP
VGGLGRAAWMR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU45800
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CDK12 from DU45682 cloned into NheI-NsiI of DU45774