cDNA Clone SYT1

• Synonyms: P65, SVP65, SYT
• Plasmid: pGEX6P-1 TEVb SYT1 270-end K326A K328A
• Parent Plasmid: pGEX6P1
• Expressed: GST-TEVb-SYT1 270-end K326A K328A
• Genbank: NM_001135805.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGAAAACCTGTATTTTCAGATGGAGCAAGAGAAATTGGGTGATATCTGCTTCTCCCTTCGCTACGTACCTACTGCTGGTAAGCTGACTGTTGTCATTCTGGAGGCAAAGAACCTGAAGAAGATGGATGTGGGTGGCTTATCCGATCCTTATGTGAAGATTCATCTGATGCAGAATGGTAAGAGGCTGAAGGCGAAAGCGACAACAATTAAAAAGAACACACTTAACCCCTACTACAATGAGTCATTCAGCTTTGAAGTACCTTTTGAACAAATCCAGAAAGTGCAGGTGGTGGTAACTGTTTTGGACTATGACAAGATTGGCAAGAACGATGCCATCGGCAAAGTCTTTGTGGGCTACAACAGCACCGGCGCGGAGCTGCGACACTGGTCAGACATGCTGGCCAACCCCAGGCGACCTATTGCCCAGTGGCACACCCTGCAGGTAGAGGAGGAAGTTGATGCCATGCTGGCCGTCAAGAAGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSENLYFQMEQEKLGDICFSL
RYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKAKATTI
KKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGY
NSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44835
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 270-end, K326/328A. Cleavage at starting Methionine