cDNA Clone HA-IKKbeta S177A/S181A

• Synonyms: IKBKB
• Plasmid: pRetrox tight HA IKKb S177A S181A
• Parent Plasmid: pRetroX tight puro
• Expressed: HA IKKb S177A S181A
• Genbank: NP_001547.1
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGAGCTGGTCACCTTCCCTGACAACGCAAACATGTGGGGCCTG
GGAAATGAAAGAGCGCCTTGGGACAGGGGGATTTGGAAATGTCATCCGAT
GGCACAATCAGGAAACAGGTGAGCAGATTGCCATCAAGCAGTGCCGGCAG
GAGCTCAGCCCCCGGAACCGAGAGCGGTGGTGCCTGGAGATCCAGATCAT
GAGAAGGCTGACCCACCCCAATGTGGTGGCTGCCCGAGATGTCCCTGAGG
GGATGCAGAACTTGGCGCCCAATGACCTGCCCCTGCTGGCCATGGAGTAC
TGCCAAGGAGGAGATCTCCGGAAGTACCTGAACCAGTTTGAGAACTGCTG
TGGTCTGCGGGAAGGTGCCATCCTCACCTTGCTGAGTGACATTGCCTCTG
CGCTTAGATACCTTCATGAAAACAGAATCATCCATCGGGATCTAAAGCCA
GAAAACATCGTCCTGCAGCAAGGAGAACAGAGGTTAATACACAAAATTAT
TGACCTAGGATATGCCAAGGAGCTGGATCAGGGCgctCTTTGCACAgCAT
TCGTGGGGACCCTGCAGTACCTGGCCCCAGAGCTACTGGAGCAGCAGAAG
TACACAGTGACCGTCGACTACTGGAGCTTCGGCACCCTGGCCTTTGAGTG
CATCACGGGCTTCCGGCCCTTCCTCCCCAACTGGCAGCCCGTGCAGTGGC
ATTCAAAAGTGCGGCAGAAGAGTGAGGTGGACATTGTTGTTAGCGAAGAC
TTGAATGGAACGGTGAAGTTTTCAAGCTCTTTACCCTACCCCAATAATCT
TAACAGTGTCCTGTCTGAGCGACTGGAGAAGTGGCTGCAACTGATGCTGA
TGTGGCACCCCCGACAGAGGGGCACGGATCCCACGTATGGGCCCAATGGC
TGCTTCAAGGCCCTGGATGACATCTTAAACTTAAAGCTGGTTCATATCTT
GAACATGGTCACGGGCACCATCCACACCTACCCTGTGACAGAGGATGAGA
GTCTGCAGAGCTTGAAGGCCAGAATCCAACAGGACACGGGCATCCCAGAG
GAGGACCAGGAGCTGCTGCAGGAAGCGGGCCTGGCGTTGATCCCCGATAA
GCCTGCCACTCAGTGTATTTCAGACGGCAAGTTAAATGAGGGCCACACAT
TGGACATGGATCTTGTTTTTCTCTTTGACAACAGTAAAATCACCTATGAG
ACTCAGATCTCCCCACGGCCCCAACCTGAAAGTGTCAGCTGTATCCTTCA
AGAGCCCAAGAGGAATCTCGCCTTCTTCCAGCTGAGGAAGGTGTGGGGCC
AGGTCTGGCACAGCATCCAGACCCTGAAGGAAGATTGCAACCGGCTGCAG
CAGGGACAGCGAGCCGCCATGATGAATCTCCTCCGAAACAACAGCTGCCT
CTCCAAAATGAAGAATTCCATGGCTTCCATGTCTCAGCAGCTCAAGGCCA
AGTTGGATTTCTTCAAAACCAGCATCCAGATTGACCTGGAGAAGTACAGC
GAGCAAACCGAGTTTGGGATCACATCAGATAAACTGCTGCTGGCCTGGAG
GGAAATGGAGCAGGCTGTGGAGCTCTGTGGGCGGGAGAACGAAGTGAAAC
TCCTGGTAGAACGGATGATGGCTCTGCAGACCGACATTGTGGACTTACAG
AGGAGCCCCATGGGCCGGAAGCAGGGGGGAACGCTGGACGACCTAGAGGA
GCAAGCAAGGGAGCTGTACAGGAGACTAAGGGAAAAACCTCGAGACCAGC
GAACTGAGGGTGACAGTCAGGAAATGGTACGGCTGCTGCTTCAGGCAATT
CAGAGCTTCGAGAAGAAAGTGCGAGTGATCTATACGCAGCTCAGTAAAAC
TGTGGTTTGCAAGCAGAAGGCGCTGGAACTGTTGCCCAAGGTGGAAGAGG
TGGTGAGCTTAATGAATGAGGATGAGAAGACTGTTGTCCGGCTGCAGGAG
AAGCGGCAGAAGGAGCTCTGGAATCTCCTGAAGATTGCTTGTAGCAAGGT
CCGTGGTCCTGTCAGTGGAAGCCCGGATAGCATGAATGCCTCTCGACTTA
GCCAGCCTGGGCAGCTGATGTCTCAGCCCTCCACGGCCTCCAACAGCTTA
CCTGAGCCAGCCAAGAAGAGTGAAGAACTGGTGGCTGAAGCACATAACCT
CTGCACCCTGCTAGAAAATGCCATACAGGACACTGTGAGGGAACAAGACC
AGAGTTTCACGGCCCTAGACTGGAGCTGGTTACAGACGGAAGAAGAAGAG
CACAGCTGCCTGGAGCAGGCCTCATGATGTGGGGGGACTCGACCCCCTGA
CATGGGGCAGCCCATAGCAGGCCTTGTGCGGTGGGGGGACTCGACCCCCT
GACATGGGGCTGCCTGGAGCAGGCCTCATGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSGEFGAAASWSPSLTTQTCGAWEMKERLGTGGFGNVIRW
HNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEG
MQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDIASA
LRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGALCTAF
VGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWH
SKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLSERLEKWLQLMLM
WHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTEDES
LQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEGHTL
DMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRKVWGQ
VWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAK
LDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKL
LVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQR
TEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEV
VSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLS
QPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQ
SFTALDWSWLQTEEEEHSCLEQAS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU46081
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: please insert HA-IKKbeta into pRETRO-X-tight. We also need both S177 and S181 mutated to Ala. Thank you.