cDNA Clone EIF4G3

• Synonyms: eIF-4G 3, eIF4G 3, eIF4GII
• Plasmid: pSCB EIF4G3 tv3 1-1221 template only
• Parent Plasmid: pSC-B
• Genbank: NM_003760.4
• Species: Human

Sequence of Insert

CTCGAGATGAATTCACAACCTCAAACCCGTTCTCCGTTTTTCCAGAGGCCTCAAATACAGCCTCCTAGAGCTACCATCCCGAACAGCAGTCCTTCCATTCGTCCTGGTGCACAGACACCCACTGCAGTGTACCAGGCTAATCAGCACATCATGATGGTTAACCATCTGCCCATGCCGTACCCAGTGCCCCAGGGGCCTCAGTACTGTATACCACAGTACCGTCATAGTGGCCCTCCTTATGTTGGGCCCCCCCAACAATATCCAGTTCAACCACCGGGGCCAGGTCCTTTTTATCCTGGACCAGGACCTGGGGACTTCCCCAATGCTTATGGAACGCCTTTTTACCCAAGTCAGCCGGTGTATCAGTCAGCACCTATCATAGTGCCTACGCAGCAACAGCCGCCTCCAGCCAAGAGAGAGAAAAAAACTATAAGAATTCGGGATCCAAACCAGGGAGGTAAAGACATAACAGAGGAGATTATGTCTGGAGGTGGCAGCAGAAATCCTACTCCACCCATAGGAAGACCCACGTCCACACCTACTCCTCCTCAGCAGCTGCCCAGCCAGGTCCCCGAGCACAGCCCTGTGGTTTATGGGACTGTGGAGAGCGCTCATCTTGCTGCCAGCACCCCTGTCACTGCAGCTAGCGACCAGAAGCAAGAGGAGAAGCCAAAACCAGATCCAGTGTTAAAGTCTCCTTCCCCAGTCCTTAGGCTAGTCCTCAGTGGAGAGAAGAAAGAACAAGAAGGCCAGACATCTGAAACTACTGCAATAGTATCCATAGCAGAGCTTCCTCTGCCTCCATCACCTACCACTGTTTCTTCTGTTGCTCGAAGTACAATTGCAGCCCCCACCTCTTCTGCTCTTAGTAGCCAACCAATATTCACCACTGCTATAGATGACAGATGTGAACTCTCATCCCCAAGAGAAGACACAATTCCTATACCCAGCCTCACATCTTGCACAGAAACATCAGACCCTTTACCAACAAATGAAAATGATGATGATATATGCAAGAAACCCTGTAGTGTAGCACCTAATGATATTCCACTGGTTTCTAGTACTAACCTAATTAATGAAATAAATGGAGTTAGCGAAAAATTATCAGCCACGGAGAGCATTGTGGAAATAGTAAAACAGGAAGTATTGCCATTGACTCTTGAATTGGAGATTCTCGAAAATCCCCCAGAAGAAATGAAACTGGAGTGTATCCCAGCTCCCATCACCCCTTCCACAGTTCCTTCCTTTCCTCCAACTCCTCCAACTCCTCCAGCTTCTCCTCCTCACACTCCAGTCATTGTTCCTGCTGCTGCCACTACTGTTAGTTCTCCGAGTGCTGCCATCACAGTCCAGAGAGTCCTAGAGGAGGACGAGAGCATAAGAACTTGCCTTAGTGAAGATGCAAAAGAGATTCAGAACAAAATAGAGGTAGAAGCAGATGGGCAAACAGAAGAGATTTTGGATTCTCAAAACTTAAATTCAAGAAGGAGCCCTGTCCCAGCTCAAATAGCTATAACTGTACCAAAGACATGGAAGAAACCAAAAGATCGGACCCGAACCACTGAAGAGATGTTAGAGGCAGAATTGGAGCTTAAAGCTGAAGAGGAGCTTTCCATTGACAAAGTACTTGAATCTGAACAAGATAAAATGAGCCAGGGGTTTCATCCTGAAAGAGACCCCTCTGACCTAAAAAAAGTGAAAGCTGTGGAAGAAAATGGAGAAGAAGCTGAGCCAGTACGTAATGGTGCTGAGAGTGTTTCTGAGGGTGAAGGAATAGATGCTAATTCAGGCTCCACAGATAGTTCTGGTGATGGGGTTACATTTCCATTTAAACCAGAATCCTGGAAGCCTACTGATACTGAAGGTAAGAAGCAGTATGACAGGGAGTTTCTGCTGGACTTCCAGTTCATGCCTGCCTGTATACAAAAACCAGAGGGCCTGCCTCCTATCAGTGATGTGGTTCTTGACAAGATCAACCAACCCAAATTGCCAATGCGAACTCTGGATCCTCGAATTTTGCCTCGAGGACCAGACTTTACACCAGCCTTTGCTGATTTTGGAAGGCAGACACCTGGTGGAAGAGGCGTACCTTTGTTGAATGTTGGGTCACGAAGATCTCAACCTGGCCAAAGAAGAGAACCCAGAAAGATCATCACAGTTTCTGTAAAAGAAGATGTACACCTGAAAAAGGCAGAAAATGCCTGGAAGCCAAGCCAAAAACGAGACAGCCAAGCCGATGATCCCGAAAACATTAAAACCCAGGAGCTTTTTAGAAAAGTTCGAAGTATCTTAAATAAATTGACACCACAGATGTTCAATCAACTGATGAAGCAAGTGTCAGGACTTACTGTTGACACAGAGGAGCGGCTGAAAGGAGTTATTGACCTGGTCTTTGAGAAGGCTATTGATGAACCCAGTTTCTCTGTGGCTTACGCAAACATGTGTCGATGTCTAGTAACGCTGAAAGTACCCATGGCAGACAAGCCTGGTAACACAGTGAATTTCCGGAAGCTGCTACTGAACCGTTGCCAGAAGGAGTTTGAAAAAGATAAAGCAGATGATGATGTCTTTGAGAAGAAGCAGAAAGAACTTGAGGCTGCCAGTGCTCCAGAGGAGAGGACAAGGCTTCATGATGAACTGGAAGAAGCCAAGGACAAAGCCCGGCGGAGATCCATTGGCAACATCAAGTTTATTGGAGAACTCTTTAAACTCAAAATGCTGACTGAAGCCATCATGCATGACTGTGTGGTGAAGCTGCTAAAGAACCATGATGAAGAATCCCTGGAGTGCCTGTGTCGCCTGCTCACCACCATTGGCAAAGACTTGGACTTTGAAAAAGCAAAGCCACGTATGGACCAGTACTTTAATCAGATGGAGAAAATTGTGAAAGAAAGAAAAACCTCATCTAGGATTCGGTTCATGCTTCAAGATGTTATAGACCTAAGGCTGTGCAATTGGGTATCTCGAAGAGCAGATCAAGGGCCTAAAACTATCGAACAGATTCACAAAGAGGCTAAAATAGAAGAACAAGAAGAGCAAAGGAAGGTCCAGCAACTCATGACCAAAGAGAAGAGAAGACCAGGTGTCCAGAGAGTGGACGAAGGTGGGTGGAACACTGTACAAGGGGCCAAGAACAGTCGGGTACTGGACCCCTCAAAATTCCTAAAAATCACTAAGCCTACAATTGATGAAAAAATTCAGCTGGTACCTAAAGCACAGCTAGGCAGCTGGGGAAAAGGCAGCAGTGGTGGAGCAAAGGCAAGTGAGACTGATGCCTTACGGTCAAGTGCTTCCAGTTTAAACAGATTCTCTGCCCTGCAACCTCCAGCACCCTCAGGGTCCACGCCATCCACGCCTGTAGAGTTTGATTCCCGAAGGACCTTAACTAGTCGTGGAAGTATGGGCAGGGAGAAGAATGACAAGCCCCTTCCATCTGCAACAGCTCGGCCAAATACTTTCATGAGGGGTGGCAGCAGTAAAGACCTGCTAGACAATCAGTCTCAAGAAGAGCAGCGGAGAGAGATGCTGGAGACCGTGAAGCAGCTCACAGGAGGTGTGGATGTGGAGAGGAACAGCACTGAGGCTGAGCGAAATAAAACAAGGGAGTCAGCAAAACCAGAAATTTCAGCAATGTCAGCTCATGACAAGGCTGCATTATCAGAAGAGGAGTCTGAGGATAACTAAGCGGCCGC

Amino Acid Sequence

MNSQPQTRSPFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMM
VNHLPMPYPVPQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPG
PGDFPNAYGTPFYPSQPVYQSAPIIVPTQQQPPPAKREKKTIRIRDPNQG
GKDITEEIMSGGGSRNPTPPIGRPTSTPTPPQQLPSQVPEHSPVVYGTVE
SAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQT
SETTAIVSIAELPLPPSPTTVSSVARSTIAAPTSSALSSQPIFTTAIDDR
CELSSPREDTIPIPSLTSCTETSDPLPTNENDDDICKKPCSVAPNDIPLV
SSTNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLE
CIPAPITPSTVPSFPPTPPTPPASPPHTPVIVPAAATTVSSPSAAITVQR
VLEEDESIRTCLSEDAKEIQNKIEVEADGQTEEILDSQNLNSRRSPVPAQ
IAITVPKTWKKPKDRTRTTEEMLEAELELKAEEELSIDKVLESEQDKMSQ
GFHPERDPSDLKKVKAVEENGEEAEPVRNGAESVSEGEGIDANSGSTDSS
GDGVTFPFKPESWKPTDTEGKKQYDREFLLDFQFMPACIQKPEGLPPISD
VVLDKINQPKLPMRTLDPRILPRGPDFTPAFADFGRQTPGGRGVPLLNVG
SRRSQPGQRREPRKIITVSVKEDVHLKKAENAWKPSQKRDSQADDPENIK
TQELFRKVRSILNKLTPQMFNQLMKQVSGLTVDTEERLKGVIDLVFEKAI
DEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFEKDKA
DDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELF
KLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMD
QYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQGPKTIEQIH
KEAKIEEQEEQRKVQQLMTKEKRRPGVQRVDEGGWNTVQGAKNSRVLDPS
KFLKITKPTIDEKIQLVPKAQLGSWGKGSSGGAKASETDALRSSASSLNR
FSALQPPAPSGSTPSTPVEFDSRRTLTSRGSMGREKNDKPLPSATARPNT
FMRGGSSKDLLDNQSQEEQRREMLETVKQLTGGVDVERNSTEAERNKTRE
SAKPEISAMSAHDKAALSEEESEDN*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU44840
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Residues 1-1221 of variant 3, template only. Contains internal XhoI site, will need to reamplify SalI-NotI.