cDNA Clone FBXL4

• Plasmid: pcDNA5 FRT/TO FBXL4 1-370 FS*15 3FLAG
• Parent Plasmid: pcDNA5 FRT/TO C-3FLAG
• Expressed: FBXL4 1-370 FS*15 3FLAG
• Genbank: Q9UKA2
• Tag: 3FLAG
• Species: Human

Sequence of Insert

CTCGAGATGTCACCGGTCTTTCCCATGTTAACAGTTCTGACCATGTTTTA
TTATATATGCCTTCGGCGCCGAGCCAGGACAGCTACAAGAGGAGAAATGA
TGAACACCCATAGAGCTATAGAATCAAACAGCCAGACTTCCCCTCTCAAT
GCAGAGGTAGTCCAGTATGCCAAAGAAGTAGTGGATTTCAGTTCCCATTA
TGGAAGTGAGAATAGTATGTCCTATACTATGTGGAATTTGGCTGGTGTAC
CAAATGTATTCCCAAGTTCTGGTGACTTTACTCAGACAGCTGTGTTTCGA
ACTTATGGGACATGGTGGGATCAGTGTCCTAGTGCTTCCTTGCCATTCAA
GAGGACGCCACCTAATTTTCAGAGCCAGGACTATGTGGAACTTACTTTTG
AACAACAGGTGTATCCTACAGCTGTACATGTTCTAGAAACCTATCATCCC
GGAGCAGTCATTAGAATTCTCGCTTGTTCTGCAAATCCTTATTCCCCAAA
TCCACCAGCTGAAGTAAGATGGGAGATTCTTTGGTCAGAGAGACCTACGA
AGGTGAATGCTTCCCAAGCTCGCCAGTTTAAACCTTGTATTAAGCAGATA
AATTTCCCCACAAATCTTATACGACTGGAAGTAAATAGTTCTCTTCTGGA
ATATTACACTGAATTAGATGCAGTTGTGCTACATGGTGTGAAGGACAAGC
CAGTGCTTTCTCTCAAGACTTCACTTATTGACATGAATGATATAGAAGAT
GATGCCTATGCAGAAAAGGATGGTTGTGGAATGGACAGTCTTAACAAAAA
GTTTAGCAGTGCTGTCCTCGGGGAAGGGCCAAATAATGGGTATTTTGATA
AACTACCTTATGAGCTTATTCAGCTGATTCTGAATCATCTTACACTACCA
GACCTGTGTAGATTAGCACAGACTTGCAAACTACTGAGCCAGCATTGCTG
TGATCCTCTGCAATACATCCACCTCAATCTGCAACCATACTGGGCAAAAC
TAGATGACACTTCTCTGGAATTTCTACAGTCTCGCTGCACTCTTGTCCAG
TGGCTTAATTTATCTTGGACTGCAATAGAGGCTTCATCTCTGTTGCAGGA
TTTAGCAGGTTTCGCGGCCGC

Amino Acid Sequence

MSPVFPMLTVLTMFYYICLRRRARTATRGEMMNTHRAIESNSQTSPLNAE
VVQYAKEVVDFSSHYGSENSMSYTMWNLAGVPNVFPSSGDFTQTAVFRTY
GTWWDQCPSASLPFKRTPPNFQSQDYVELTFEQQVYPTAVHVLETYHPGA
VIRILACSANPYSPNPPAEVRWEILWSERPTKVNASQARQFKPCIKQINF
PTNLIRLEVNSSLLEYYTELDAVVLHGVKDKPVLSLKTSLIDMNDIEDDA
YAEKDGCGMDSLNKKFSSAVLGEGPNNGYFDKLPYELIQLILNHLTLPDL
CRLAQTCKLLSQHCCDPLQYIHLNLQPYWAKLDDTSLEFLQSRCTLVQWL
NLSWTAIEASSLLQDLAGFAAADYKDDDDKDYKDDDDKDYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU47085
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: in pcDNA5frtTO with C-terminal FLAG; c. 1067_1067 del --> G356A and introduction of a stop codon at 371, hence the 1-370 fragment for c-ter tagging; ; parental DU43547