cDNA Clone FBXL4 (mouse)

• Plasmid: pGEX blunt TEV-ms FBXL4
• Parent Plasmid: pGEX TEV
• Expressed: GST-ms-FBXL4 [wild-type]
• Genbank: Q8BH70
• Tag: GST

Sequence of Insert

ATGTCACCAGTCTTTCCCATGTTAACAGTTCTGACCATGTTTTATTATATGTGCCTTCGGCGTCGAGCCAGGACAGCTACAAGAGGAGATATGATGAACAGCCATAGGACTATAGTGTCAAACAGCCGGACTTCCCCTCTCAATGCAGAGGTGGTCCAGTATGCTAAAGAAGTGGTGGATTTCAGTTCCCATTACGGCAGTGAGAACAGTATGTCCTATACCATGTGGAATTTGGCAGGTGTACCAAATGTCTTCCCAAGTTCTGGTGACTTCACGCAGACGGCTGTGTTTCGAACTTATGGGACGTGGTGGGATCAGTGTCCTAGTGCTTCCTTGCCATTCAGAAGGACACCGTCCAGTTTCCAGAGCCAGGACTATGTGGAACTGACTTTTGAGCAGCAAGTGTACCCCACGGCTGTCCATGTGCTGGAGACTTACCATCCTGGAGCAGTCATCAGAATTCTGGCCTGCTCAGCAAATCCCTACTCCCCAAATCCTCCAGCTGAAGTAAGATGGGAGATTCTTTGGTCAGAGAGACCTATGAAGGTGAATGCTTCCCAGGCTCGTCAGTTTAAACCTTGTATTAAGCAAATAAATTTCCCTACAAACCTCATACGACTGGAAGTCAATAGTTCTCTTCTGGATTATTACACCGAACTAGATGCAGTTGTGCTGCATGGTACGAAGGACAAGCCACTGCTCTCTCTCAAGACTGCTCTTGTAGACATGAATGATCTAGAAGATGACGACTATGAAGAGAAGGATGGCTGTGAGATGGATGCTCTTAACAAAAAGTTTAGCAGTGCTGCCCTTGGGGATGGGCCACATAATGGGTATTTTGACAAACTACCATATGAGCTCATTCAACTGATTCTGAATCATCTTTCACTACCAGACCTGTGTAGATTAGCCCAGACTTGCAGGCTTCTCCACCAGCATTGCTGTGATCCTCTGCAATATATCCACCTCAACCTGCAGCCATACTGGGCCCGGCTGGACGACACGTCACTGGAGTTCCTACAGGCTCGCTGTGTGCTTGTCCAGTGGCTTAATCTGTCTTGGACTGGCAACAGAGGGTTCATCTCTGTATCAGGATTTAGCAGGTTTCTAAAGGTGTGTGGGTCTGAGTTAGTACGCCTTGAGTTATCCTGCAGCCACTTTCTTAATGACACTTGCTTGGAGGTTATTTCTGAAATGTGCCCAAATCTACAAGACTTAAATCTCTCATCCTGCGATAAACTCCCACCTCAGGCTTTTGGCCATATTGCCAAGTTATGCAGCCTCAAGAGGCTTGTTCTCTATCGGACAAAAGTAGAGCAAACAGCACTACTGAGCATTTTGAACTTCTGTGCAGAGCTTCAGCACCTCAGCCTGGGCAGCTGTGTGATGATTGAAGATTATGATGTGATAGCCAGCATGATAGGAGCCAAGTGTAAAAACCTGCGGACTTTGGATCTGTGGAGATGCAAGAATATCACCGAGAATGGGATAGCAGAGTTGGCTTCTGGCTGTGTTCTTCTTGAGGAACTTGACCTTGGCTGGTGCCCCACCCTTCAGAGCAGCACTGGGTGCTTTGTCAGACTGGCACGCCAGCTCCCAAACTTGCAGAAACTCTTTCTTACAGCTAACAGGTCTGTGTGTGACACAGACATAGAAGAACTGGCATCTAATTGTACCAGATTACAGCAACTGGACATATTAGGAACAAGAATGGTAAGTCCAGCATCCTTGAGAAAACTCCTGGAATCTTGCAAAGATCTTTCCCTACTTGACGTGTCCTTCTGTTCCCAGATTGATAACAAGGCTGTGTTAGAGCTCAACGCGAGCTTTCCGAAAGTCTTCATAAAGAAGAGCTTTACTCAATGA

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDENLYFQMSPVFPMLTVLTMFYYMCLRRRARTATRGDMMNSHRTIVSNSRTSPLNAEVVQYAKEVVDFSSHYGSENSMSYTMWNLAGVPNVFPSSGDFTQTAVFRTYGTWWDQCPSASLPFRRTPSSFQSQDYVELTFEQQVYPTAVHVLETYHPGAVIRILACSANPYSPNPPAEVRWEILWSERPMKVNASQARQFKPCIKQINFPTNLIRLEVNSSLLDYYTELDAVVLHGTKDKPLLSLKTALVDMNDLEDDDYEEKDGCEMDALNKKFSSAALGDGPHNGYFDKLPYELIQLILNHLSLPDLCRLAQTCRLLHQHCCDPLQYIHLNLQPYWARLDDTSLEFLQARCVLVQWLNLSWTGNRGFISVSGFSRFLKVCGSELVRLELSCSHFLNDTCLEVISEMCPNLQDLNLSSCDKLPPQAFGHIAKLCSLKRLVLYRTKVEQTALLSILNFCAELQHLSLGSCVMIEDYDVIASMIGAKCKNLRTLDLWRCKNITENGIAELASGCVLLEELDLGWCPTLQSSTGCFVRLARQLPNLQKLFLTANRSVCDTDIEELASNCTRLQQLDILGTRMVSPASLRKLLESCKDLSLLDVSFCSQIDNKAVLELNASFPKVFIKKSFTQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU42964
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: in pGEX with TEV that permits removal of tag with no overhanging residues