cDNA Clone FBXL4

• Plasmid: pET-His-SUMO FBXL4
• Parent Plasmid: pET15b 6His SUMO
• Expressed: His-SUMO-FBXL4
• Genbank: Q9UKA2
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

ATGTCACCGGTCTTTCCCATGTTAACAGTTCTGACCATGTTTTATTATATATGCCTTCGGCGCCGAGCCAGGACAGCTACAAGAGGAGAAATGATGAACACCCATAGAGCTATAGAATCAAACAGCCAGACTTCCCCTCTCAATGCAGAGGTAGTCCAGTATGCCAAAGAAGTAGTGGATTTCAGTTCCCATTATGGAAGTGAGAATAGTATGTCCTATACTATGTGGAATTTGGCTGGTGTACCAAATGTATTCCCAAGTTCTGGTGACTTTACTCAGACAGCTGTGTTTCGAACTTATGGGACATGGTGGGATCAGTGTCCTAGTGCTTCCTTGCCATTCAAGAGGACGCCACCTAATTTTCAGAGCCAGGACTATGTGGAACTTACTTTTGAACAACAGGTGTATCCTACAGCTGTACATGTTCTAGAAACCTATCATCCCGGAGCAGTCATTAGAATTCTCGCTTGTTCTGCAAATCCTTATTCCCCAAATCCACCAGCTGAAGTAAGATGGGAGATTCTTTGGTCAGAGAGACCTACGAAGGTGAATGCTTCCCAAGCTCGCCAGTTTAAACCTTGTATTAAGCAGATAAATTTCCCCACAAATCTTATACGACTGGAAGTAAATAGTTCTCTTCTGGAATATTACACTGAATTAGATGCAGTTGTGCTACATGGTGTGAAGGACAAGCCAGTGCTTTCTCTCAAGACTTCACTTATTGACATGAATGATATAGAAGATGATGCCTATGCAGAAAAGGATGGTTGTGGAATGGACAGTCTTAACAAAAAGTTTAGCAGTGCTGTCCTCGGGGAAGGGCCAAATAATGGGTATTTTGATAAACTACCTTATGAGCTTATTCAGCTGATTCTGAATCATCTTACACTACCAGACCTGTGTAGATTAGCACAGACTTGCAAACTACTGAGCCAGCATTGCTGTGATCCTCTGCAATACATCCACCTCAATCTGCAACCATACTGGGCAAAACTAGATGACACTTCTCTGGAATTTCTACAGTCTCGCTGCACTCTTGTCCAGTGGCTTAATTTATCTTGGACTGGCAATAGAGGCTTCATCTCTGTTGCAGGATTTAGCAGGTTTCTGAAGGTTTGTGGATCCGAATTAGTACGCCTTGAATTGTCTTGCAGCCACTTTCTTAATGAAACTTGCTTAGAAGTTATTTCTGAGATGTGTCCAAATCTACAGGCCTTAAATCTCTCCTCCTGTGATAAGCTACCACCTCAAGCTTTCAACCACATTGCCAAGTTATGCAGCCTTAAACGACTTGTTCTCTATCGAACAAAAGTAGAGCAAACAGCACTGCTCAGCATTTTGAACTTCTGTTCAGAGCTTCAGCACCTCAGTTTAGGCAGTTGTGTCATGATTGAAGACTATGATGTGATAGCTAGCATGATAGGAGCCAAGTGTAAAAAACTCCGGACCCTGGATCTGTGGAGATGTAAGAATATTACTGAGAATGGAATAGCAGAACTGGCTTCTGGGTGTCCACTACTGGAGGAGCTTGACCTTGGCTGGTGCCCAACTCTGCAGAGCAGCACCGGGTGCTTCACCAGACTGGCACACCAGCTCCCAAACTTGCAAAAACTCTTTCTTACAGCTAATAGATCTGTGTGTGACACAGACATTGATGAATTGGCATGTAATTGTACCAGGTTACAGCAGCTGGACATATTAGGAACAAGAATGGTAAGTCCGGCATCCTTAAGAAAACTCCTGGAATCTTGTAAAGATCTTTCTTTACTTGATGTGTCCTTCTGTTCGCAGATTGATAACAGAGCTGTGCTAGAACTGAATGCAAGCTTTCCAAAAGTGTTCATAAAAAAGAGCTTTACTCAGTGA

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMSPVFPMLTVLTMFYYICLRRRARTATRGEMMNTHRAIESNSQTSPLNAEVVQYAKEVVDFSSHYGSENSMSYTMWNLAGVPNVFPSSGDFTQTAVFRTYGTWWDQCPSASLPFKRTPPNFQSQDYVELTFEQQVYPTAVHVLETYHPGAVIRILACSANPYSPNPPAEVRWEILWSERPTKVNASQARQFKPCIKQINFPTNLIRLEVNSSLLEYYTELDAVVLHGVKDKPVLSLKTSLIDMNDIEDDAYAEKDGCGMDSLNKKFSSAVLGEGPNNGYFDKLPYELIQLILNHLTLPDLCRLAQTCKLLSQHCCDPLQYIHLNLQPYWAKLDDTSLEFLQSRCTLVQWLNLSWTGNRGFISVAGFSRFLKVCGSELVRLELSCSHFLNETCLEVISEMCPNLQALNLSSCDKLPPQAFNHIAKLCSLKRLVLYRTKVEQTALLSILNFCSELQHLSLGSCVMIEDYDVIASMIGAKCKKLRTLDLWRCKNITENGIAELASGCPLLEELDLGWCPTLQSSTGCFTRLAHQLPNLQKLFLTANRSVCDTDIDELACNCTRLQQLDILGTRMVSPASLRKLLESCKDLSLLDVSFCSQIDNRAVLELNASFPKVFIKKSFTQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU42962
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: the linker between His Sumo has been removed and with it the BamHI site used for cloning