cDNA Clone EIF4G3

• Synonyms: eIF-4G 3, eIF4G 3, eIF4GII
• Plasmid: pCMV5 FLAG EIF4G3
• Parent Plasmid: pCMV5D FLAG
• Expressed: FLAG-EIF4G3
• Genbank: NM_003760.4
• Tag: FLAG
• Species: Human

Sequence of Insert

GTCGACATGAATTCACAACCTCAAACCCGTTCTCCGTTTTTCCAGAGGCCTCAAATACAGCCTCCTAGAGCTACCATCCCGAACAGCAGTCCTTCCATTCGTCCTGGTGCACAGACACCCACTGCAGTGTACCAGGCTAATCAGCACATCATGATGGTTAACCATCTGCCCATGCCGTACCCAGTGCCCCAGGGGCCTCAGTACTGTATACCACAGTACCGTCATAGTGGCCCTCCTTATGTTGGGCCCCCCCAACAATATCCAGTTCAACCACCGGGGCCAGGTCCTTTTTATCCTGGACCAGGACCTGGGGACTTCCCCAATGCTTATGGAACGCCTTTTTACCCAAGTCAGCCGGTGTATCAGTCAGCACCTATCATAGTGCCTACGCAGCAACAGCCGCCTCCAGCCAAGAGAGAGAAAAAAACTATAAGAATTCGGGATCCAAACCAGGGAGGTAAAGACATAACAGAGGAGATTATGTCTGGAGGTGGCAGCAGAAATCCTACTCCACCCATAGGAAGACCCACGTCCACACCTACTCCTCCTCAGCAGCTGCCCAGCCAGGTCCCCGAGCACAGCCCTGTGGTTTATGGGACTGTGGAGAGCGCTCATCTTGCTGCCAGCACCCCTGTCACTGCAGCTAGCGACCAGAAGCAAGAGGAGAAGCCAAAACCAGATCCAGTGTTAAAGTCTCCTTCCCCAGTCCTTAGGCTAGTCCTCAGTGGAGAGAAGAAAGAACAAGAAGGCCAGACATCTGAAACTACTGCAATAGTATCCATAGCAGAGCTTCCTCTGCCTCCATCACCTACCACTGTTTCTTCTGTTGCTCGAAGTACAATTGCAGCCCCCACCTCTTCTGCTCTTAGTAGCCAACCAATATTCACCACTGCTATAGATGACAGATGTGAACTCTCATCCCCAAGAGAAGACACAATTCCTATACCCAGCCTCACATCTTGCACAGAAACATCAGACCCTTTACCAACAAATGAAAATGATGATGATATATGCAAGAAACCCTGTAGTGTAGCACCTAATGATATTCCACTGGTTTCTAGTACTAACCTAATTAATGAAATAAATGGAGTTAGCGAAAAATTATCAGCCACGGAGAGCATTGTGGAAATAGTAAAACAGGAAGTATTGCCATTGACTCTTGAATTGGAGATTCTCGAAAATCCCCCAGAAGAAATGAAACTGGAGTGTATCCCAGCTCCCATCACCCCTTCCACAGTTCCTTCCTTTCCTCCAACTCCTCCAACTCCTCCAGCTTCTCCTCCTCACACTCCAGTCATTGTTCCTGCTGCTGCCACTACTGTTAGTTCTCCGAGTGCTGCCATCACAGTCCAGAGAGTCCTAGAGGAGGACGAGAGCATAAGAACTTGCCTTAGTGAAGATGCAAAAGAGATTCAGAACAAAATAGAGGTAGAAGCAGATGGGCAAACAGAAGAGATTTTGGATTCTCAAAACTTAAATTCAAGAAGGAGCCCTGTCCCAGCTCAAATAGCTATAACTGTACCAAAGACATGGAAGAAACCAAAAGATCGGACCCGAACCACTGAAGAGATGTTAGAGGCAGAATTGGAGCTTAAAGCTGAAGAGGAGCTTTCCATTGACAAAGTACTTGAATCTGAACAAGATAAAATGAGCCAGGGGTTTCATCCTGAAAGAGACCCCTCTGACCTAAAAAAAGTGAAAGCTGTGGAAGAAAATGGAGAAGAAGCTGAGCCAGTACGTAATGGTGCTGAGAGTGTTTCTGAGGGTGAAGGAATAGATGCTAATTCAGGCTCCACAGATAGTTCTGGTGATGGGGTTACATTTCCATTTAAACCAGAATCCTGGAAGCCTACTGATACTGAAGGTAAGAAGCAGTATGACAGGGAGTTTCTGCTGGACTTCCAGTTCATGCCTGCCTGTATACAAAAACCAGAGGGCCTGCCTCCTATCAGTGATGTGGTTCTTGACAAGATCAACCAACCCAAATTGCCAATGCGAACTCTGGATCCTCGAATTTTGCCTCGAGGACCAGACTTTACACCAGCCTTTGCTGATTTTGGAAGGCAGACACCTGGTGGAAGAGGCGTACCTTTGTTGAATGTTGGGTCACGAAGATCTCAACCTGGCCAAAGAAGAGAACCCAGAAAGATCATCACAGTTTCTGTAAAAGAAGATGTACACCTGAAAAAGGCAGAAAATGCCTGGAAGCCAAGCCAAAAACGAGACAGCCAAGCCGATGATCCCGAAAACATTAAAACCCAGGAGCTTTTTAGAAAAGTTCGAAGTATCTTAAATAAATTGACACCACAGATGTTCAATCAACTGATGAAGCAAGTGTCAGGACTTACTGTTGACACAGAGGAGCGGCTGAAAGGAGTTATTGACCTGGTCTTTGAGAAGGCTATTGATGAACCCAGTTTCTCTGTGGCTTACGCAAACATGTGTCGATGTCTAGTAACGCTGAAAGTACCCATGGCAGACAAGCCTGGTAACACAGTGAATTTCCGGAAGCTGCTACTGAACCGTTGCCAGAAGGAGTTTGAAAAAGATAAAGCAGATGATGATGTCTTTGAGAAGAAGCAGAAAGAACTTGAGGCTGCCAGTGCTCCAGAGGAGAGGACAAGGCTTCATGATGAACTGGAAGAAGCCAAGGACAAAGCCCGGCGGAGATCCATTGGCAACATCAAGTTTATTGGAGAACTCTTTAAACTCAAAATGCTGACTGAAGCCATCATGCATGACTGTGTGGTGAAGCTGCTAAAGAACCATGATGAAGAATCCCTGGAGTGCCTGTGTCGCCTGCTCACCACCATTGGCAAAGACTTGGACTTTGAAAAAGCAAAGCCACGTATGGACCAGTACTTTAATCAGATGGAGAAAATTGTGAAAGAAAGAAAAACCTCATCTAGGATTCGGTTCATGCTTCAAGATGTTATAGACCTAAGGCTGTGCAATTGGGTATCTCGAAGAGCAGATCAAGGGCCTAAAACTATCGAACAGATTCACAAAGAGGCTAAAATAGAAGAACAAGAAGAGCAAAGGAAGGTCCAGCAACTCATGACCAAAGAGAAGAGAAGACCAGGTGTCCAGAGAGTGGACGAAGGTGGGTGGAACACTGTACAAGGGGCCAAGAACAGTCGGGTACTGGACCCCTCAAAATTCCTAAAAATCACTAAGCCTACAATTGATGAAAAAATTCAGCTGGTACCTAAAGCACAGCTAGGCAGCTGGGGAAAAGGCAGCAGTGGTGGAGCAAAGGCAAGTGAGACTGATGCCTTACGGTCAAGTGCTTCCAGTTTAAACAGATTCTCTGCCCTGCAACCTCCAGCACCCTCAGGGTCCACGCCATCCACGCCTGTAGAGTTTGATTCCCGAAGGACCTTAACTAGTCGTGGAAGTATGGGCAGGGAGAAGAATGACAAGCCCCTTCCATCTGCAACAGCTCGGCCAAATACTTTCATGAGGGGTGGCAGCAGTAAAGACCTGCTAGACAATCAGTCTCAAGAAGAGCAGCGGAGAGAGATGCTGGAGACCGTGAAGCAGCTCACAGGAGGTGTGGATGTGGAGAGGAACAGCACTGAGGCTGAGCGAAATAAAACAAGGGAGTCAGCAAAACCAGAAATTTCAGCAATGTCAGCTCATGACAAGGCTGCATTATCAGAAGAGGAGTCTGAGGATAACTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSFEEFASLDMNSQPQTRSPFFQRPQIQPPRATIPNSSPSI
RPGAQTPTAVYQANQHIMMVNHLPMPYPVPQGPQYCIPQYRHSGPPYVGP
PQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQSAPIIVPTQQQ
PPPAKREKKTIRIRDPNQGGKDITEEIMSGGGSRNPTPPIGRPTSTPTPP
QQLPSQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSP
SPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIAA
PTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNEN
DDDICKKPCSVAPNDIPLVSSTNLINEINGVSEKLSATESIVEIVKQEVL
PLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPTPPASPPHTPVI
VPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQT
EEILDSQNLNSRRSPVPAQIAITVPKTWKKPKDRTRTTEEMLEAELELKA
EEELSIDKVLESEQDKMSQGFHPERDPSDLKKVKAVEENGEEAEPVRNGA
ESVSEGEGIDANSGSTDSSGDGVTFPFKPESWKPTDTEGKKQYDREFLLD
FQFMPACIQKPEGLPPISDVVLDKINQPKLPMRTLDPRILPRGPDFTPAF
ADFGRQTPGGRGVPLLNVGSRRSQPGQRREPRKIITVSVKEDVHLKKAEN
AWKPSQKRDSQADDPENIKTQELFRKVRSILNKLTPQMFNQLMKQVSGLT
VDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTV
NFRKLLLNRCQKEFEKDKADDDVFEKKQKELEAASAPEERTRLHDELEEA
KDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCR
LLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRL
CNWVSRRADQGPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRVD
EGGWNTVQGAKNSRVLDPSKFLKITKPTIDEKIQLVPKAQLGSWGKGSSG
GAKASETDALRSSASSLNRFSALQPPAPSGSTPSTPVEFDSRRTLTSRGS
MGREKNDKPLPSATARPNTFMRGGSSKDLLDNQSQEEQRREMLETVKQLT
GGVDVERNSTEAERNKTRESAKPEISAMSAHDKAALSEEESEDN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44869
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Residues 1-1221 of variant 3.