cDNA Clone TRPM6

• Synonyms: CHAK2, HMGX, HOMG, HOMG1, HSH
• Plasmid: pGEX TRPM6 1-800
• Parent Plasmid: pGEX6P1
• Expressed: GST-TRPM6 1-800
• Genbank: NM_017662.4
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAAAGAACAACCTGTCTTGGAGCGCTTGCAGTCCCAGAAATCCTGGATTAAAGGAGTATTTGACAAGAGAGAATGTAGCACAATCATACCCAGCTCAAAAAATCCTCACAGATGTACTCCAGTATGCCAAGTCTGCCAGAATTTAATCAGGTGTTACTGTGGCCGACTGATTGGAGACCATGCTGGGATAGATTATTCCTGGACCATCTCAGCTGCCAAGGGTAAAGAAAGTGAACAATGGTCTGTTGAAAAGCACACAACGAAAAGCCCAACAGATACTTTTGGCACGATTAATTTCCAAGATGGAGAGCACACCCATCATGCCAAGTATATTAGAACTTCTTATGATACAAAACTGGATCATCTGTTACATTTAATGTTGAAAGAGTGGAAAATGGAACTGCCCAAGCTTGTGATCTCAGTCCATGGGGGCATCCAGAACTTTACTATGCCCTCTAAATTTAAAGAGATTTTCAGCCAAGGTTTGGTTAAAGCTGCAGAGACAACCGGTGCGTGGATAATAACTGAAGGCATCAATACAGGAGTGTCCAAGCATGTTGGGGATGCCTTGAAATCCCATTCCTCTCATTCCTTGAGAAAAATCTGGACAGTTGGAATCCCTCCTTGGGGTGTCATTGAGAACCAGAGAGACCTTATTGGAAAAGATGTGGTGTGCCTGTACCAGACTCTGGATAACCCCCTCAGCAAGCTCACAACACTCAACAGCATGCACTCGCACTTCATCCTGTCTGATGATGGGACCGTGGGCAAGTATGGAAATGAAATGAAGCTCAGAAGGAACCTGGAGAAGTACCTCTCTCTGCAGAAAATACACTGCCGCTCAAGACAAGGCGTGCCGGTCGTGGGGCTGGTGGTGGAAGGCGGTCCCAACGTCATCCTGTCAGTGTGGGAGACTGTCAAGGACAAGGACCCAGTGGTGGTGTGTGAGGGTACAGGTAGGGCGGCTGACCTCCTGGCCTTCACACACAAACACCTGGCAGATGAAGGGATGCTGCGACCTCAGGTGAAAGAGGAGATCATCTGCATGATTCAGAACACTTTCAACTTTAGTCTTAAACAGTCCAAGCACCTTTTCCAAATTCTAATGGAGTGTATGGTTCACAGGGATTGTATTACCATATTTGATGCTGACTCTGAAGAGCAGCAAGACCTGGACTTAGCAATCCTAACAGCTTTGCTGAAGGGCACAAATTTATCAGCGTCAGAGCAATTAAATCTGGCAATGGCTTGGGACAGGGTGGACATTGCCAAGAAACATATCCTAATTTATGAACAACACTGGAAGCCTGATGCCCTGGAACAAGCAATGTCAGATGCTTTAGTGATGGATCGGGTGGATTTTGTGAAGCTCTTAATAGAATATGGAGTGAACCTCCATCGCTTTCTTACCATCCCTCGACTGGAAGAGCTCTACAATACAAAACAAGGACCTACTAATACACTCTTGCATCATCTCGTCCAAGATGTGAAACAGCATACCCTTCTTTCAGGCTACCGAATAACCTTGATTGACATTGGATTAGTAGTAGAATACCTCATTGGTAGAGCATATCGCAGCAACTACACTAGAAAACATTTCAGAGCCCTCTACAACAACCTCTACAGAAAATACAAGCACCAGAGACACTCCTCAGGAAATAGAAATGAGTCTGCAGAAAGTACGCTGCACTCCCAGTTCATTAGAACTGCACAGCCATACAAATTCAAGGAAAAGTCTATAGTCCTTCATAAATCAAGGAAGAAGTCAAAAGAACAAAATGTATCAGATGACCCTGAGTCTACTGGCTTTCTTTACCCTTACAATGACCTGCTGGTTTGGGCTGTGCTGATGAAAAGGCAGAAGATGGCTATGTTCTTCTGGCAGCATGGAGAGGAGGCCACGGTTAAAGCCGTGATTGCGTGTATCCTCTACCGGGCAATGGCCCATGAAGCTAAGGAGAGTCACATGGTGGATGATGCCTCAGAAGAGTTGAAGAATTACTCAAAACAGTTTGGCCAGCTGGCTCTGGACTTGTTGGAGAAGGCATTCAAGCAGAATGAGCGCATGGCCATGACGCTGTTGACGTATGAACTCAGGAACTGGAGCAATTCGACCTGCCTTAAACTGGCCGTGTCGGGAGGATTACGACCCTTTGTTTCACATACTTGTACCCAGATGCTACTGACAGACATGTGGATGGGGAGGCTGAAAATGAGGAAAAACTCTTGGTTAAAGATTATTATAAGCATTATTTTACCACCCACCATTTTGACACTGGAATTTAAAAGCAAAGCTGAGATGTCACATGTTCCCCAGTCCCAGGACTTCCAATTTATGTGGTATTACAGTGACCAGAACGCCAGCAGTTCCAAAGAAAGTGCTTCTGTGAAAGAGTATTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMKEQPVLERLQSQKSWIKG
VFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWT
ISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTK
LDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAET
TGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDL
IGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNL
EKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGT
GRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQIL
MECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWD
RVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRF
LTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDIGLVVEY
LIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQFIR
TAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMK
RQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYS
KQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPF
VSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMS
HVPQSQDFQFMWYYSDQNASSSKESASVKEY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44879
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 1-800; ; All TRPM6 plasmids MUST be grown at 30 degrees C or less to prevent recombination.