cDNA Clone ARHGEF12

• Synonyms: LARG, PRO2792
• Plasmid: pSCB ARHGEF12 Bam Not
• Parent Plasmid: pSC-B
• Genbank: NM_015313.2
• Species: Human

Sequence of Insert

GGATCCATGAGTGGCACACAGTCTACTATCACCGACAGGTTTCCCCTCAAAAAACCTATAAGGCATGGAAGTATTTTGAACCGAGAGTCACCAACAGATAAGAAGCAGAAAGTTGAGCGCATTGCATCACATGATTTTGACCCCACAGATAGCTCCTCCAAGAAGACAAAGTCTAGTTCAGAGGAGAGTAGATCCGAGATATATGGTCTTGTTCAGCGTTGCGTAATCATCCAGAAAGATGACAATGGATTTGGGCTGACGGTCAGTGGAGACAATCCAGTCTTCGTACAGTCTGTCAAAGAAGATGGAGCAGCCATGCGGGCTGGAGTACAGACAGGTGATCGAATCATCAAGGTGAATGGAACTCTGGTGACTCATTCAAATCATCTGGAGGTGGTGAAGCTAATCAAATCTGGTTCCTATGTAGCTCTCACTGTTCAGGGACGCCCACCTGGGTCGCCCCAGATTCCACTTGCCGACTCTGAAGTAGAGCCGTCAGTCATTGGACATATGTCTCCCATCATGACATCTCCTCATTCACCTGGAGCATCTGGGAATATGGAGAGAATCACTAGTCCTGTGCTCATGGGGGAGGAAAACAATGTGGTTCATAACCAGAAAGTAGAAATTCTGAGAAAAATGTTACAGAAAGAACAGGAACGGCTACAGTTATTGCAGGAAGATTACAACCGAACACCTGCCCAAAGATTGCTAAAAGAGATCCAAGAGGCCAAGAAACACATTCCTCAGCTGCAAGAGCAGTTATCCAAAGCCACAGGCTCTGCTCAGGATGGAGCTGTAGTTACACCCTCCAGACCTTTAGGGGACACCCTAACAGTCAGTGAGGCAGAAACAGATCCTGGAGATGTACTGGGCAGGACTGACTGTAGCAGTGGAGATGCTTCTCGGCCCAGTAGTGACAATGCAGATAGTCCCAAGAGTGGCCCAAAAGAGAGAATTTATCTAGAGGAAAACCCAGAGAAAAGTGAAACAATTCAGGACACTGACACTCAATCACTTGTCGGAAGTCCCTCAACCCGTATAGCACCTCATATTATTGGAGCAGAAGATGATGATTTTGGTACTGAACATGAACAGATCAATGGACAGTGCAGCTGTTTCCAGAGCATTGAATTACTAAAATCTCGCCCGGCTCATTTGGCTGTTTTCTTACACCATGTAGTTTCACAATTTGACCCTGCGACTTTGCTCTGTTATCTCTATTCAGACCTGTATAAACATACCAATTCCAAAGAAACTCGTCGCATCTTCCTTGAGTTTCATCAGTTCTTTCTAGATCGATCAGCACACCTGAAAGTTTCTGTTCCTGATGAAATGTCTGCAGATCTAGAAAAGAGAAGACCTGAGCTCATTCCTGAGGATCTGCATCGCCACTATATCCAAACTATGCAAGAAAGAGTCCATCCAGAAGTTCAAAGGCACTTAGAAGATTTTCGGCAGAAACGTAGTATGGGACTGACCTTGGCTGAAAGCGAGCTGACTAAACTTGATGCAGAGCGAGACAAGGACCGATTGACTTTGGAGAAGGAGCGGACATGTGCAGAACAGATTGTTGCCAAAATTGAAGAAGTATTGATGACTGCTCAGGCTGTAGAGGAAGATAAGAGCTCCACCATGCAGTATGTTATTCTCATGTATATGAAGCATTTGGGAGTAAAAGTGAAAGAGCCTCGAAATTTGGAGCACAAACGGGGTCGGATTGGATTTCTTCCCAAAATCAAGCAAAGTATGAAGAAAGATAAAGAAGGGGAAGAAAAAGGGAAGCGAAGAGGATTCCCCAGCATCCTGGGACCCCCACGGAGACCAAGCCGTCATGACAACAGTGCAATTGGCAGAGCCATGGAACTACAGAAGGCGCGCCACCCTAAGCACTTATCCACACCCTCATCTGTGAGTCCTGAACCTCAGGACTCTGCCAAGTTGCGCCAGAGTGGGTTAGCAAATGAAGGAACAGACGCTGGATACCTGCCTGCCAATTCCATGTCTTCTGTAGCTTCAGGGGCCTCTTTTTCCCAGGAAGGAGGGAAAGAGAATGATACAGGATCAAAGCAAGTTGGAGAAACATCAGCACCTGGAGACACCTTAGATGGCACACCTCGTACTCTCAATACTGTCTTTGATTTCCCACCACCTCCATTAGACCAAGTGCAGGAGGAGGAATGTGAAGTAGAAAGGGTGACTGAACATGGGACACCAAAGCCCTTTCGAAAGTTTGACAGTGTAGCTTTTGGAGAAAGTCAAAGTGAGGATGAACAATTTGAAAATGACTTAGAGACAGACCCACCCAACTGGCAGCAGCTTGTTAGTCGAGAAGTGTTACTGGGACTAAAACCTTGTGAAATCAAAAGACAGGAAGTGATTAATGAATTGTTCTACACTGAAAGAGCTCATGTTCGAACACTGAAGGTTCTTGATCAAGTGTTCTATCAGCGAGTATCCAGAGAAGGAATTCTGTCACCCTCAGAGCTACGGAAAATTTTTTCAAACTTGGAAGATATTCTTCAACTTCATATTGGATTGAATGAACAAATGAAGGCTGTTCGAAAGAGAAATGAGACCTCTGTTATCGATCAGATTGGGGAAGATTTGCTGACATGGTTCAGCGGACCAGGAGAGGAGAAATTGAAACATGCTGCTGCTACCTTTTGCAGTAACCAACCTTTCGCCCTGGAAATGATCAAATCTCGTCAGAAAAAGGATTCTCGATTTCAGACTTTTGTGCAAGATGCTGAAAGTAATCCACTGTGTCGTCGTCTTCAACTGAAGGATATTATTCCCACTCAAATGCAAAGGCTTACTAAGTACCCACTTCTGTTGGATAATATTGCCAAATACACAGAATGGCCAACAGAAAGGGAGAAGGTGAAGAAAGCTGCAGATCACTGTCGTCAGATCTTAAATTATGTAAATCAGGCTGTCAAGGAGGCAGAAAACAAGCAGCGCCTAGAAGATTATCAGCGTCGCCTTGATACCTCCAGCCTGAAGTTGTCAGAGTACCCAAATGTTGAAGAGCTCAGGAATTTGGATTTAACAAAAAGGAAGATGATTCATGAAGGGCCATTGGTTTGGAAGGTGAATAGAGATAAAACTATTGATTTATACACGTTGCTGCTGGAAGACATTCTTGTATTGTTACAAAAGCAGGATGATAGACTGGTTTTAAGGTGTCATAGTAAGATTCTGGCATCTACAGCTGATAGCAAACACACGTTTAGCCCTGTCATTAAGTTGAGTACAGTGTTGGTTCGACAAGTGGCAACAGATAACAAAGCTTTATTCGTCATTTCCATGTCAGACAATGGCGCTCAGATTTATGAACTGGTGGCACAGACAGTTTCTGAAAAGACTGTCTGGCAGGACCTAATCTGTCGGATGGCTGCATCAGTGAAGGAGCAATCCACAAAGCCAATTCCATTACCACAGTCAACACCTGGCGAAGGAGATAATGATGAAGAAGATCCTTCAAAATTAAAAGAGGAGCAGCATGGCATTTCAGTCACTGGTTTGCAGAGTCCAGACAGAGATTTGGGATTAGAATCTACCTTAATATCGTCAAAACCTCAGTCCCATTCACTGAGTACCTCTGGGAAATCAGAGGTACGTGATCTGTTTGTGGCTGAGAGACAGTTTGCAAAGGAACAACATACAGATGGGACACTAAAGGAAGTTGGAGAAGATTATCAAATCGCAATCCCAGATTCACACCTGCCTGTCTCAGAAGAACGGTGGGCATTGGATGCACTAAGAAATTTGGGTTTGTTGAAGCAGTTGCTGGTGCAACAGCTAGGTTTGACTGAGAAGAGCGTTCAGGAAGACTGGCAACATTTCCCAAGATACAGAACAGCCTCTCAGGGGCCGCAGACAGACAGTGTCATCCAGAACTCTGAAAATATTAAGGCCTATCATTCTGGTGAAGGACATATGCCCTTTAGAACTGGAACTGGTGACATTGCAACTTGTTACAGTCCACGGACTTCAACTGAATCTTTTGCTCCACGGGATTCAGTGGGACTGGCACCCCAGGATAGCCAGGCAAGTAACATTTTAGTAATGGACCACATGATTATGACCCCAGAGATGCCTACCATGGAGCCAGAAGGGGGTCTTGATGACAGTGGAGAGCACTTTTTTGATGCCCGTGAAGCACATAGTGATGAGAATCCATCAGAAGGTGATGGAGCAGTTAACAAGGAAGAGAAGGATGTTAATTTACGCATCTCAGGAAACTATTTGATCCTTGATGGCTATGACCCAGTGCAGGAGAGTTCCACAGATGAGGAGGTTGCTTCCTCACTTACCCTGCAGCCCATGACAGGCATCCCTGCTGTGGAATCCACCCACCAGCAGCAACATTCTCCTCAGAATACTCACTCCGATGGGGCAATTTCACCATTCACCCCCGAATTTCTGGTCCAGCAGCGCTGGGGAGCTATGGAGTATTCCTGTTTTGAGATCCAGAGTCCCTCCTCTTGTGCAGATTCACAGAGCCAGATCATGGAGTACATTCATAAGATAGAGGCTGACCTTGAACACTTAAAGAAGGTGGAGGAAAGTTACACCATTCTTTGCCAAAGGCTGGCTGGATCAGCCCTCACAGACAAGCACTCAGATAAAAGTTAGCGGCCGC

Amino Acid Sequence

MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTDSS
SKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKED
GAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPG
SPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNV
VHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQ
EQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDAS
RPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHI
IGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPAT
LLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEK
RRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTK
LDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILM
YMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSI
LGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSG
LANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTL
DGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGE
SQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTER
AHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMK
AVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKS
RQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKY
TEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLK
LSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLL
QKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVI
SMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPG
EGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTS
GKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWAL
DALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNS
ENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQA
SNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAV
NKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVES
THQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQ
SQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU44901
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee