cDNA Clone cyclin K

• Plasmid: pGEX6P-1 cyclin K 6HIS
• Parent Plasmid: pGEX6P1
• Expressed: GST-cyclin K-His6
• Genbank: NM_0010094.2
• Tag: GST
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCTATGAAGGAGAATAAAGAAAATTCAAGCCCTTCAGTAACTTCAGC
AAACCTGGACCACACAAAGCCATGTTGGTACTGGGATAAGAAAGACTTGG
CTCATACACCCTCACAACTTGAAGGACTTGATCCAGCCACCGAGGCCCGG
TACCGCCGAGAGGGCGCTCGGTTCATCTTTGATGTGGGCACACGTTTGGG
GCTACACTATGATACCCTGGCAACTGGAATAATTTATTTTCATCGCTTCT
ATATGTTTCATTCCTTCAAGCAATTCCCAAGATATGTGACAGGAGCCTGT
TGCCTCTTTCTGGCTGGGAAAGTAGAAGAAACACCAAAAAAATGTAAAGA
TATCATCAAAACAGCTCGTAGTTTATTAAATGATGTACAATTTGGCCAGT
TTGGAGATGACCCAAAGGAGGAAGTAATGGTTCTGGAGAGAATCTTACTG
CAGACCATCAAGTTTGATTTACAGGTAGAACATCCATACCAGTTCCTACT
AAAATATGCAAAGCAACTCAAAGGTGATAAAAACAAAATTCAAAAGTTGG
TTCAAATGGCATGGACATTTGTAAATGACAGTCTCTGCACCACCTTGTCA
CTGCAGTGGGAACCAGAGATCATAGCAGTAGCAGTGATGTATCTCGCAGG
ACGTTTGTGCAAATTTGAAATACAAGAATGGACCTCCAAACCCATGTATA
GGAGATGGTGGGAGCAGTTTGTTCAAGATGTCCCGGTCGACGTTTTGGAA
GACATCTGCCACCAAATCCTGGATCTTTACTCACAAGGAAAACAACAGAT
GCCTCATCACACCCCCCATCAGCTGCAACAGCCCCCATCTCTTCAGCCTA
CACCACAAGTGCCGCAAGTACAGCAGTCACAGCCGTCTCAAAGCTCCGAA
CCATCCCAGCCCCAGCAGAAGGACCCCCAGCAACCAGCCCAGCAGCAGCA
GCCAGCCCAACAGCCCAAGAAACCCTCTCCGCAGCCCAGTTCTCCCCGAC
AGGTTAAGCGAGCCGTGGTTGTTTCTCCCAAAGAAGAGAACAAAGCAGCA
GAACCACCACCACCTAAAATCCCCAAAATTGAGACCACTCATCCACCGTT
GCCTCCAGCCCACCCACCTCCAGACCGGAAGCCTCCCCTCGCTGCTGCCT
TAGGTGAGGCTGAGCCGCCGGGCCCTGTGGATGCCACTGACCTCCCCAAA
GTCCAGATTCCCCCTCCGGCCCACCCGGCCCCTGTGCACCAGCCACCGCC
GCTGCCACACCGGCCCCCGCCCCCACCCCCCTCCAGCTACATGACCGGGA
TGTCCACCACCAGCTCCTACATGTCTGGAGAGGGCTACCAGAGCCTGCAG
TCCATGATGAAGACCGAGGGACCCTCCTACGGTGCCCTGCCCCCCGCCTA
CGGCCCACCTGCACACCTGCCCTACCACCCCCATGTCTACCCGCCCAACC
CGCCCCCGCCACCTGTGCCTCCTCCCCCAGCCTCCTTCCCCCCACCTGCC
ATCCCACCCCCTACTCCTGGCTACCCCCCACCCCCACCCACCTACAACCC
CAACTTCCCACCCCCACCCCCACGCCTCCCGCCTACCCACGCAGTCCCCC
CTCATCCTCCTCCAGGGTTGGGCCTGCCGCCAGCCAGCTACCCACCTCCT
GCCGTCCCCCCTGGAGGACAGCCTCCTGTGCCCCCGCCCATTCCCCCACC
CGGCATGCCTCCAGTTGGGGGGCTGGGGCGGGCAGCCTGGATGAGACATC
ATCACCATCACCATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMKENKENSSPSVTSANLDH
TKPCWYWDKKDLAHTPSQLEGLDPATEARYRREGARFIFDVGTRLGLHYD
TLATGIIYFHRFYMFHSFKQFPRYVTGACCLFLAGKVEETPKKCKDIIKT
ARSLLNDVQFGQFGDDPKEEVMVLERILLQTIKFDLQVEHPYQFLLKYAK
QLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQWEPEIIAVAVMYLAGRLCK
FEIQEWTSKPMYRRWWEQFVQDVPVDVLEDICHQILDLYSQGKQQMPHHT
PHQLQQPPSLQPTPQVPQVQQSQPSQSSEPSQPQQKDPQQPAQQQQPAQQ
PKKPSPQPSSPRQVKRAVVVSPKEENKAAEPPPPKIPKIETTHPPLPPAH
PPPDRKPPLAAALGEAEPPGPVDATDLPKVQIPPPAHPAPVHQPPPLPHR
PPPPPPSSYMTGMSTTSSYMSGEGYQSLQSMMKTEGPSYGALPPAYGPPA
HLPYHPHVYPPNPPPPPVPPPPASFPPPAIPPPTPGYPPPPPTYNPNFPP
PPPRLPPTHAVPPHPPPGLGLPPASYPPPAVPPGGQPPVPPPIPPPGMPP
VGGLGRAAWMRHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46165
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GST with PreScission to cleave the GST tag