cDNA Clone CCDC99

• Synonyms: Spindly, hSpindly, protein Spindly
• Plasmid: pCMV5-FLAG CCDC99 K117R
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-CCDC99 K117R
• Genbank: NM_017785.4
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCATGGAGGCAGATATAATCACAAATCTTCGATGCAGGCTCAAAGAGGCTGAAGAAGAGCGACTAAAAGCTGCACAGTATGGTTTACAACTAGTAGAGAGTCAAAATGAATTACAGAATCAATTGGATAAATGTCGTAATGAAATGATGACCATGACTGAGAGTTATGAACAAGAAAAATATACCCTTCAAAGAGAAGTTGAACTCAAGAGTCGAATGTTAGAAAGTTTGAGCTGCGAATGTGAAGCTATTAAACAACAACAAAAAATGCACCTGGAGAAATTGGAAGAACAACTAAGCAGAAGCCATGGACAGGAAGTGAATGAACTAAAAACTAAGATAGAAAAACTGAGAGTGGAATTAGATGAAGCCAGGCTTAGTGAAAAGCAGCTGAAGCACCAAGTAGATCATCAGAAGGAACTCCTCTCTTGTAAATCAGAGGAACTGCGCGTAATGTCTGAACGTGTGCAGGAAAGCATGTCTTCAGAGATGCTGGCTCTTCAAATTGAGCTGACAGAAATGGAGAGTATGAAGACCACCCTCAAAGAAGAAGTGAATGAACTACAATACAGACAAGAACAGCTAGAACTTCTTATTACTAACCTAATGCGCCAGGTAGACCGGCTTAAAGAGGAAAAAGAGGAGCGAGAGAAAGAAGCAGTTTCTTACTATAATGCCCTAGAGAAAGCTCGTGTAGCAAATCAAGATCTTCAGGTACAGTTGGACCAGGCACTCCAGCAAGCCTTGGATCCCAATAGTAAAGGCAACTCTTTGTTTGCAGAGGTGGAAGATCGAAGGGCAGCAATGGAACGTCAGCTTATCAGTATGAAAGTCAAGTATCAGTCACTAAAGAAGCAAAATGTATTTAACAGAGAACAGATGCAGAGAATGAAGTTACAAATTGCCACGTTGCTACAGATGAAAGGGTCTCAAACTGAATTTGAGCAGCAGGAACGGTTGCTTGCCATGTTGGAGCAGAAGAATGGTGAAATAAAACATCTTTTAGGTGAAATTAGAAATCTGGAGAAATTTAAGAATTTATATGACAGTATGGAATCTAAGCCTTCAGTCGACTCTGGTACTCTGGAAGATAACACCTATTATACAGATTTACTTCAGATGAAGCTGGATAACTTAAACAAAGAAATTGAAAGCACTAAAGGTGAATTGTCCATACAGCGAATGAAAGCATTATTTGAGAGCCAGCGGGCTCTAGATATTGAGCGAAAACTTTTTGCAAATGAAAGATGCCTCCAGCTTTCAGAAAGTGAAAATATGAAACTGAGAGCTAAACTAGATGAATTGAAACTAAAATATGAACCTGAAGAGACAGTTGAAGTGCCTGTACTGAAAAAGAGGCGTGAGGTGCTCCCTGTGGATATAACCACCGCTAAAGATGCATGTGTCAACAACAGTGCTCTCGGGGGAGAAGTTTATCGATTACCGCCTCAGAAAGAGGAGACACAGTCCTGCCCTAACAGTTTAGAAGATAACAACTTGCAATTAGAAAAATCAGTTTCTATACACACACCAGTAGTCAGTCTCTCTCCTCACAAAAATCTGCCCGTGGATATGCAGCTGAAGAAGGAAAAGAAATGTGTGAAACTCATAGGAGTTCCCGCTGACGCTGAGGCCTTAAGTGAAAGAAGTGGAAACACCCCTAACTCTCCCAGGTTAGCTGCTGAATCAAAGCTTCAAACAGAAGTTAAAGAAGGAAAAGAAACTTCAAGCAAATTGGAAAAAGAAACTTGTAAGAAATCACACCCTATTCTATATGTGTCTTCTAAATCTACTCCAGAGACCCAGTGCCCTCAACAGTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSPEFMEADIITNLRCRLKEAEEERLKAAQYGLQLVESQNE
LQNQLDKCRNEMMTMTESYEQEKYTLQREVELKSRMLESLSCECEAIKQQ
QKMHLEKLEEQLSRSHGQEVNELKTKIEKLRVELDEARLSEKQLKHQVDH
QKELLSCKSEELRVMSERVQESMSSEMLALQIELTEMESMKTTLKEEVNE
LQYRQEQLELLITNLMRQVDRLKEEKEEREKEAVSYYNALEKARVANQDL
QVQLDQALQQALDPNSKGNSLFAEVEDRRAAMERQLISMKVKYQSLKKQN
VFNREQMQRMKLQIATLLQMKGSQTEFEQQERLLAMLEQKNGEIKHLLGE
IRNLEKFKNLYDSMESKPSVDSGTLEDNTYYTDLLQMKLDNLNKEIESTK
GELSIQRMKALFESQRALDIERKLFANERCLQLSESENMKLRAKLDELKL
KYEPEETVEVPVLKKRREVLPVDITTAKDACVNNSALGGEVYRLPPQKEE
TQSCPNSLEDNNLQLEKSVSIHTPVVSLSPHKNLPVDMQLKKEKKCVKLI
GVPADAEALSERSGNTPNSPRLAAESKLQTEVKEGKETSSKLEKETCKKS
HPILYVSSKSTPETQCPQQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44910
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: K117R. Also contains Y508H and L586S known variations, these are benign according to ensembl; ; All CCDC99 plasmids MUST be grown at 30 degrees C or less to prevent recombination.