cDNA Clone C9orf84

• Plasmid: pcDNA5D FRT/TO GFP C9ORF84 TV2 L1032R
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP C9orf84 tv2 L1032R
• Genbank: NM_001080551.1 GI:124028518 with t243c (silent to kill BamH1)
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGTCAGAGACTCTGGGGGATGAATTGGAGATATTAAGAGGTAAAATGATGCAGAGAAGACCAAGGTCAGCAGAAGTCAAATACTTCTATTTCTTTAAAATTTTGCTTAGGCTACGCCTGGCTATTTTGAAGTATTTATTTATTGATGATAAAGGAATACTTTTTGTAAGTAGTAGAAAACACCTACCAACTTTGCCTACTCTCTTGAGTAGACTAAAACTGTTTTTGGTAAAGGACCCTCTTTTAGATTTCAAAGGACAGATCTTCACAGAAGCTAATTTTTCCAGGGAATGTTTCTCTCTTCAAGAAACTTTGGAAGCTTTTGTGAAAGAAGATTTTTGTATGGATAAAGTGAACTTTTGTCAAGAGAAACTAGAAGATACAATATGTTTAAATGAGCCGTCAAGTTTTCTTATTGAGTATGAATTCTTAATACCTCCAAGCCTCAAACCAGAAATTGATATTCCATCACTCTCAGAACTGAAGGAGTTATTAAACCCAGTGCCAGAAATAATAAACTATGTAGATGAAAAGGAAAAGCTTTTTGAAAGAGATCTTACTAACAAGCATGGAATTGAGGATATCGGGGATATAAAATTCAGCTCCACAGAGATTTTGACCATTCAAAGCCAGAGTGAACCAGAAGAGTGCAGTAAACCAGGAGAGTTAGAAATGCCACTAACTCCTCTATTCCTAACATGCCAACATTCTTCAGTGAATTCATTACGTACAGAACTTCAGACATTTCCATTATCTCCGGTTTGTAAAATTAATTTGCTTACTGCTGAAGAATCAGCTAATGAATACTACATGATGTGGCAATTAGAAAGATGTAGAAGCCCTTTGAACCCATTTTTGCTTACAGTGCCAAGAATTCAAGAGCCCCACAGCCAATATTCAGTTACAGATTTGAAAAAGATATTTTCTGTTAAAGAAGAAAGCCTTGTGATTAATCTGGAAAAGGCAGAGTGGTGGAAACAAGCAGGACTAAATCTGAAAATGATGGAAACATTGGAACATCTGAATACATATTTATGTCATGATAATTTGTCTTCTAATGACACTAAAATTGAGATATTTTTGCCTACGAAAGTGCTTCAATTAGAATCATGTCTAGAACATAAAAGTCATTCTTCACCTATTGCACTTATTGATGAAAAATCTACAAATGCTCATTTATCACTTCCACAAAAGAGTCCATCTCTGGCAAAAGAAGTACCAGATCTATGTTTTTCTGATGACTATTTCTCTGATAAAGGAGCAGCAAAAGAAGAAAAACCAAAGAATGACCAAGAACCAGTAAACAGAATAATCCAAAAGAAAGAAAATAACGATCACTTTGAACTTGACTGCACAGGACCATCTATTAAATCACCTTCCTCTTCAATAATTAAAAAAGCATCTTTTGAACATGGCAAAAAACAAGAGAATGATTTGGACCTTTTGAGCGACTTTATTATGCTGCGAAATAAATATAAGACTTGCACCTCAAAGACTGAAGTCACAAACAGTGATGAAAAACATGATAAAGAAGCATGTTCTTTGACACTTCAAGAAGAAAGTCCTATTGTTCATATTAATAAAACCCTGGAGGAAATAAATCAGGAAAGGGGAACAGATAGTGTCATTGAAATTCAAGCGTCAGATAGCCAGTGCCAAGCATTTTGCCTCCTCGAAGCAGCAGCTTCTCCTATCTTAAAAAACCTTGTATCCTTGTGTACCCTCCCTACTGCTAATTGGAAATTTGCCACTGTTATTTTTGACCAAACAAGGTTTCTCTTAAAGGAACAAGAAAAAGTAGTAAGTGATGCTGTTCGCCAAGGTACAATTGATGAAAGAGAAATGACTTTCAAGCATGCCGCTCTCTTACATCTTCTGGTAACAATTAGAGATGTCCTTTTAACATGCAGCTTGGACACAGCATTGGGATATTTGTCGAAGGCAAAAGATATCTACAACAGCATTTTAGGCCCCTATTTGGGTGACATTTGGAGACAGCTGGAGATTGTACAGTTTATTAGGGGGAAAAAGCCTGAAACCAACTACAAGATACAAGAATTGCAATGTCAGATACTAAGTTGGATGCAAAGTCAACAGCAAATTAAGGTACTGATTATAATAAGAATGGACTCAGACGGTGAAAAACATTTTCTCATTAAAATTCTTAACAAAATAGAAGGTTTAACACTGACTGTCCTTCATTCAAATGAAAGAAAAGATTTTCTGGAATCTGAAGGTGTTTTAAGGGGTACAAGTTCCTGTGTAGTTGTACATAATCAATATATTGGAGCAGATTTCCCCTGGAGTAATTTCTCATTTGTGGTGGAATACAATTATGTGGAAGACTCTTGTTGGACTAAACACTGCAAAGAGTTGAATATTCCTTACATGGCCTTTAAAGTGATTCTTCCAGACACAGTTTTAGAAAGAAGCACCTTGCTGGATAGATTTGGAGGTTTTCTTTTGGAAATTCAGATTCCATATGTGTTTTTTGCATCTGAAGGACTTCTTAATACTCCAGACATACTTCAGCTGCTAGAATCCAACTATAACATCTCACTAGTAGAGAGAGGCTGCAGTGAGTCATTGAAACTCTTTGGAAGTTCAGAGTGTTATGTAGTGGTGACAATTGATGAACACACTGCCATAATTTTGCAGGATCTAGAAGAATTGAATTATGAGAAGGCATCAGACAATATCATTATGAGGCTGATGGCATTATCATTACAGTACAGATATTGTTGGATAATTTTATATACCAAAGAAACATTAAATTCAGAGTATCTGCTTACAGAAAAGACACTTCATCACCTAGCACTGATTTATGCAGCTTTGGTTTCATTTGGGCTAAACTCTGAAGAACTGGATGTAAAGCTTATAATTGCCCCAGGAGTAGAAGCAACTGCCTTGATAATTCGACAAATTGCTGACCACAGTTTAATGACCTCAAAGAGAGATCCTCATGAATGGTTGGATAAATCCTGGCTTAAAGTTTCACCATCTGAGGAAGAAATGTACTTACTTGATTTTCCATGTATTAACCCATTGGTGGCTCAGCTCATGCTAAATAAAGGACCTTCACTGCATTGGATACGATTAGCAACTCTGTGTCAACTTCAGGAACTCCTACCTGAAGTCCCAGAAAAAGTGTTAAAGCATTTTTGTAGCATCACTTCCCTATTCAAGATTGGTTCTTCTTCCATAACAAAATCACCGCAAATTTCGTCACCTCAGGAAAATAGGAATCAGATTAGTACCTTGTCTTCTCAAAGTTCAGCTTCTGATTTAGACTCTGTCATTCAAGAACATAATGAATATTATCAGTATTTAGGATTAGGAGAGACAGTGCAGGAAGACAAAACCACCATTTTGAATGACAACTCTTCCATTATGGAACTAAAAGAAATCTCAAGTTTTTTACCACCTGTGACTTCATACAATCAGACCAGCTACTGGAAAGACTCCAGCTGTAAATCTAATATAGGGCAGAATACTCCTTTTCTAATTAATATAGAATCAAGGAGACCGGCTTATAACTCCTTTCTAAACCACAGTGATTCAGAGTCAGATGTCTTTTCTTTGGGTCTAACACAAATGAACTGTGAAACTATAAAATCACCAACTGACACTCAGAAGAGAGTGTCAGTTGTCCCCCGTTTTATAAATTCTCAGAAAAGGAGAACACATGAAGCAAAAGGTTTCATAAATAAAGATGTATCGGACCCTATCTTTTCACTAGAGGGCACTCAATCTCCTCTTCATTGGAACTTTAAGAAAAATATATGGGAACAAGAGAATCACCCGTTCAACTTACAATATGGTGCACAGCAGACTGCATGTAACAAATTGTACTCTCAGAAAGGTAATTTATTCACTGATCAGCAAAAATGTCTATCAGATGAGTCTGAAGGCCTCACATGTGAAAGTTCAAAAGATGAGACTTTCTGGAGAGAATTACCATCTGTCCCCAGTTTGGATTTATTTCGTGCTTCTGATTCTAATGCAAATCAAAAAGAATTCAACAGCCTTTATTTCTACCAAAGAGCTGGAAAAAGTTTAGGACAGAAAAGGCACCATGAATCTTCATTTAACTCAGGAGACAAGGAATCATTAACAGGTTTTATGTGCTCACAACTACCACAATTCAAAAAACGACGTCTAGCATATGAAAAAGTCCCTGGTAGAGTTGATGGGCAGACTCGGCTGAGGTTTTTTTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMSETLGDELEILRGKMMQRRPRSAEVKYFYFFKILLRLRLAILKYLFIDDKGILFVSSRKHLPTLPTLLSRLKLFLVKDPLLDFKGQIFTEANFSRECFSLQETLEAFVKEDFCMDKVNFCQEKLEDTICLNEPSSFLIEYEFLIPPSLKPEIDIPSLSELKELLNPVPEIINYVDEKEKLFERDLTNKHGIEDIGDIKFSSTEILTIQSQSEPEECSKPGELEMPLTPLFLTCQHSSVNSLRTELQTFPLSPVCKINLLTAEESANEYYMMWQLERCRSPLNPFLLTVPRIQEPHSQYSVTDLKKIFSVKEESLVINLEKAEWWKQAGLNLKMMETLEHLNTYLCHDNLSSNDTKIEIFLPTKVLQLESCLEHKSHSSPIALIDEKSTNAHLSLPQKSPSLAKEVPDLCFSDDYFSDKGAAKEEKPKNDQEPVNRIIQKKENNDHFELDCTGPSIKSPSSSIIKKASFEHGKKQENDLDLLSDFIMLRNKYKTCTSKTEVTNSDEKHDKEACSLTLQEESPIVHINKTLEEINQERGTDSVIEIQASDSQCQAFCLLEAAASPILKNLVSLCTLPTANWKFATVIFDQTRFLLKEQEKVVSDAVRQGTIDEREMTFKHAALLHLLVTIRDVLLTCSLDTALGYLSKAKDIYNSILGPYLGDIWRQLEIVQFIRGKKPETNYKIQELQCQILSWMQSQQQIKVLIIIRMDSDGEKHFLIKILNKIEGLTLTVLHSNERKDFLESEGVLRGTSSCVVVHNQYIGADFPWSNFSFVVEYNYVEDSCWTKHCKELNIPYMAFKVILPDTVLERSTLLDRFGGFLLEIQIPYVFFASEGLLNTPDILQLLESNYNISLVERGCSESLKLFGSSECYVVVTIDEHTAIILQDLEELNYEKASDNIIMRLMALSLQYRYCWIILYTKETLNSEYLLTEKTLHHLALIYAALVSFGLNSEELDVKLIIAPGVEATALIIRQIADHSLMTSKRDPHEWLDKSWLKVSPSEEEMYLLDFPCINPLVAQLMLNKGPSLHWIRLATLCQLQELLPEVPEKVLKHFCSITSLFKIGSSSITKSPQISSPQENRNQISTLSSQSSASDLDSVIQEHNEYYQYLGLGETVQEDKTTILNDNSSIMELKEISSFLPPVTSYNQTSYWKDSSCKSNIGQNTPFLINIESRRPAYNSFLNHSDSESDVFSLGLTQMNCETIKSPTDTQKRVSVVPRFINSQKRRTHEAKGFINKDVSDPIFSLEGTQSPLHWNFKKNIWEQENHPFNLQYGAQQTACNKLYSQKGNLFTDQQKCLSDESEGLTCESSKDETFWRELPSVPSLDLFRASDSNANQKEFNSLYFYQRAGKSLGQKRHHESSFNSGDKESLTGFMCSQLPQFKKRRLAYEKVPGRVDGQTRLRFF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU25296
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Grow at 30c tendency to recombine; Parental Plasmid DU41455