cDNA Clone SYT1 (rat)

• Synonyms: P65, synaptotagmin 1, synaptotagmin-1
• Plasmid: pSuper Neo Phluorin SYT1 (rat)
• Parent Plasmid: pSuper neo mCerulean
• Expressed: Signal peptide-Phluorin-TEV-SYT1 (rat)
• Tag: pHluorin
• Tag: mCerulean
• Species: Rat

Sequence of Insert

AGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCTAGACTCGAGCTGCAGAGGGCCCTGCGTATGAGTGCAAGTGGGTTTTAGGACCAGGATGAGGCGGGGTGGGGGTGCCTACCTGACGACCGACCCCGACCCACTGGACAAGCACCCAACCCCCATTCCCCAAATTGCGCATCCCCTATCAGAGAGGGGGAGGGGAAACAGGATGCGGCGAGGCGCGTGCGCACTGCCAGCTTCAGCACCGCGGACAGTGCCTTCGCCCCCGCCTGGCGGCGCGCGCCACCGCCGCCTCAGCACTGAAGGCGCGCTGACGTCACTCGCCGGTCCCCCGCAAACTCCCCTTCCCGGCCACCTTGGTCGCGTCCGCGCCGCCGCCGGCCCAGCCGGACCGCACCACGCGAGGCGCGAGATAGGGGGGCACGGGCGCGACCATCTGCGCTGCGGCGCCGGCGACTCAGCGCTGCCTCAGTCTGCGGTGGGCAGCGGAGGAGTCGTGTCGTGCCTGAGAGCGCAGTCGAATTAGCTTGGTACCGAGCTCGGATCCATGAAAATCCTCGTGGCGGTGGCGGTCTTTTTTCTCGTTTCCACTCAACTGTTTGCAGAGGAAATCGGTGCCAACAAGCTTAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCTTGGCCAACACTTGTCACTACTTTAACTTATGGTGTTCAATGCTTTTCAAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACGATCACCAGGTGTACATCATGGCAGACAAACAAAAGAATGGAATCAAAGCTAACTTCAAAATTAGACACAACATTGAAGATGGAGGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGGCCCGTCCTTTTACCAGACAACCATTACCTGTTTACAACTTCTACTCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAACCGGTGACTACGACATCCCGACTACGCTCGAGAACCTGTACTTCCAGGGTGAGCTCAAGACTGTTGATGCTGACATGGTGAGTGCCAGTCATCCTGAGGCCCTGGCCGCCCCTGTCACCACTGTTGCGACCCTTGTCCCACACAATGCCACTGAGCCAGCCAGTCCTGGGGAAGGGAAGGAAGATGCCTTTTCCAAGCTGAAGCAGAAGTTTATGAATGAGCTGCATAAAATTCCATTGCCACCGTGGGCCTTAATAGCCATAGCCATAGTTGCGGTCCTTTTAGTCGTAACCTGCTGCTTTTGTGTCTGTAAGAAATGTTTGTTCAAAAAGAAAAACAAGAAGAAGGGGAAGGAAAAGGGAGGAAAGAACGCCATTAACATGAAAGACGTGAAAGACTTAGGGAAGACCATGAAGGATCAGGCCCTTAAGGATGACGATGCTGAAACCGGACTGACTGATGGAGAAGAAAAGGAAGAGCCCAAGGAAGAGGAGAAACTGGGAAAGCTCCAATATTCACTGGACTATGACTTCCAGAATAACCAGCTGTTGGTGGGAATCATCCAGGCTGCTGAACTGCCCGCCCTGGACATGGGGGGTACATCCGATCCATACGTCAAAGTCTTCCTGCTGCCTGACAAAAAGAAGAAATTTGAGACTAAAGTCCACCGGAAAACCCTCAATCCAGTCTTCAATGAACAATTTACTTTCAAGGTACCCTACTCGGAATTAGGTGGCAAAACCCTGGTGATGGCTGTGTATGACTTTGATCGCTTCTCCAAGCACGACATCATCGGAGAGTTCAAAGTTCCTATGAACACCGTGGATTTTGGCCATGTGACCGAGGAGTGGCGCGATCTCCAGAGCGCTGAGAAAGAAGAGCAAGAGAAACTGGGTGACATCTGCTTCTCCCTCCGCTACGTCCCTACTGCCGGCAAACTGACTGTTGTCATTCTGGAAGCCAAGAACCTGAAGAAGATGGATGTGGGTGGCTTATCTGATCCCTACGTGAAGATTCACCTGATGCAGAACGGTAAGAGGCTGAAGAAGAAAAAGACGACGATTAAGAAGAACACACTCAACCCCTACTACAACGAGTCCTTCAGCTTTGAAGTTCCGTTCGAGCAAATCCAGAAAGTGCAAGTGGTGGTAACTGTTTTGGACTATGACAAGATTGGCAAGAACGACGCGATCGGCAAAGTCTTCGTTGGTTACAACAGCACTGGGGCGGAGCTGCGACACTGGTCAGACATGCTGGCCAACCCCCGGCGACCCATCGCACAGTGGCACACTCTGCAGGTAGAGGAGGAGGTTGATGCCATGCTGGCTGTCAAGAAGTAAGAATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGAGCTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCCCGGG

Amino Acid Sequence

MKILVAVAVFFLVSTQLFAEEIGANKLSKGEELFTGVVPILVELDGDVNG
HKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRY
PDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
LKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKANFKIRHNIEDG
GVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEFV
TAAGITHGMDELYKTGDYDIPTTLENLYFQGELKTVDADMVSASHPEALA
APVTTVATLVPHNATEPASPGEGKEDAFSKLKQKFMNELHKIPLPPWALI
AIAIVAVLLVVTCCFCVCKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGK
TMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVG
IIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFT
FKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRD
LQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPY
VKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTV
LDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEE
EVDAMLAVKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44942
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Expresses a signal peptide-Phluorin-rSYT1 fusion from a hSYN1 derived promoter. Contains a TEV site for cleavage of rSTY1 from the n-ter Phluorin tag.; ; parent plasmid 43750