cDNA Clone MTMR15 (FAN1)

• Plasmid: pcDNA5 FRT/TO.GFP.Puro.DU FAN1 (MTMR15) C44A C47A
• Parent Plasmid: pcDNA5D FRT/TO GFP puro
• Expressed: GFP-FAN1 C44A C47A
• Genbank: NM_014967.4 GI:226246522 with t3015c (silent)
• Tag: GFP
• Species: Human

Sequence of Insert

GGAtctatgatgtcagaagggaaacctcctgacaaaaaaaggcctcgtag
aagcttatcaatcagcaagaataagaaaaaagcatctaattctattattt
cgtgttttaacaatgcaccacctgctaaacttgccGCAcccgttGCAagt
aaaatggtgcctagatatgacttaaaccggcaccttgatgaaatgtgtgc
taacaatgacttcgttcaagtggatccagggcaggttggcttaataaatt
caaatgtgtctatggtagatttaaccagtgttaccttagaagatgtaaca
cctaagaagtcaccaccaccaaagacaaatttaacccctggccaaagtga
ttcagcaaaaagggaagtaaagcagaagatcagtccctactttaaaagta
atgatgtggtgtgcaaaaatcaagatgagctgagaaatcgtagtgtgaaa
gtcatttgtttgggaagcctagcatctaaattgtccagaaaatacgtaaa
ggctaaaaaatcaatagataaggatgaagaatttgccggttctagtccac
agagttccaaatccacagttgttaagagcctgattgataactcttcagaa
attgaggacgaggatcaaattttggagaacagttctcaaaaagaaaacgt
gtttaaatgtgattctctaaaggaagagtgcattcctgaacatatggtaa
gaggaagtaaaataatggaagccgaaagccaaaaggctacccgggaatgt
gagaaatcagccctcacccctggattctcagataatgcgatcatgttatt
ctcaccagatttcactcttaggaatacattaaagtctacttcagaagaca
gtcttgtaaagcaagagtgtatcaaagaagtggttgaaaaacgtgaggca
tgtcattgtgaagaagtaaaaatgactgttgcttcagaagctaaaataca
gctgtcagattcagaggcaaaatctcatagttctgcagatgatgcttctg
catggagtaacatccaagaggctcctctgcaggatgacagttgcttaaac
aatgatatccctcacagcattcctttggagcaggggtcaagctgcaatgg
tcctggtcaaacaaccggtcatccttactaccttcggagtttccttgtgg
tgctgaaaaccgtacttgagaatgaagatgatatgttgctctttgatgag
caggagaagggaattgtaactaaattttatcagttatcagctactggtca
gaagttatatgtaaggctctttcaacgtaaattaagctggattaagatga
ccaaattagagtatgaagagattgccttagacttaacacctgtgattgaa
gaattgacgaatgcaggctttctacagacagaatctgagttgcaagaact
ctctgaagtgcttgaactcctttctgctcctgaactaaaatccctagcca
agaccttccacttggtgaatcccaatggacagaaacagcagctggtggac
gcctttctcaaattggccaaacagcgttcagtctgcacttggggcaagaa
taagcctggaattggtgcagtgattttaaaaagagccaaagccttggctg
gacagtcagtacgaatctgtaaaggccccagggctgtgttttcccgcatc
ttgctactgttttcgttgaccgactcaatggaagatgaagacgccgcttg
tggaggtcagggacagctttcaacagtcctgttggtcaacctcggccgaa
tggagtttcctagttacaccatcaatcggaaaacccacatcttccaagac
agagatgatcttatcagatatgcagcagccacgcacatgctgagtgacat
ttcttccgcaatggccaatgggaactgggaagaagctaaggagctcgctc
agtgtgcaaaaagggattggaacagactgaaaaaccacccttctctgaga
tgccacgaagatttaccactcttcctgcggtgtttcactgttgggtggat
ttatacaaggattttgtctcggtttgtggaaatactgcagagacttcaca
tgtatgaggaagccgtcagagaacttgaaagccttttgtctcagagaatt
tattgtcctgacagcagaggccgatggtgggatcgactggcccttaattt
acaccagcacttgaagcgcctggaaccgactatcaagtgcatcacagagg
ggctggcggatccggaagtcagaacgggacaccgcctttcactgtatcag
cgagccgtgcgcctgcgagagtctccgagctgtaaaaagttcaagcacct
cttccagcagctcccagaaatggctgtgcaagatgtgaaacacgtgacca
tcacaggcaggctgtgcccacagcgtgggatgtgcaagtctgtgtttgtg
atggaggccggggaggccgctgaccccaccacggtcctgtgctctgtgga
ggagctggcactggcccattacagacgcagcggttttgaccaggggattc
atggcgaagggtccaccttcagcaccctgtatggcctcctcctgtgggac
atcatcttcatggatgggattccggatgtcttcagaaacgcctgtcaggc
attccccctggacttgtgcacagacagcttcttcacaagcagacgcccag
cccttgaggccaggctgcagctgattcatgatgcccccgaggagagcctg
cgggcctgggtggcagccacgtggcatgagcaggaaggcagagtggcttc
ccttgtcagctgggatcgcttcacgtctcttcagcaagctcaggatcttg
tctcctgcctggggggccctgtgctcagtggtgtgtgcaggcacctggct
gctgactttcgacactgtcgagggggcctccccgacctggtggtgtggaa
ctcccagagccgtcactttaagctggtggaagttaaaggccccaatgatc
gtctttcacataagcagatgatctggctggctgaactgcagaagctgggg
gctgaagtagaagtctgccacgtggttgcagttggagctaagagccaaag
ccttagctaagcggccgc

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMMSEG
KPPDKKRPRRSLSISKNKKKASNSIISCFNNAPPAKLAAPVASKMVPRYD
LNRHLDEMCANNDFVQVDPGQVGLINSNVSMVDLTSVTLEDVTPKKSPPP
KTNLTPGQSDSAKREVKQKISPYFKSNDVVCKNQDELRNRSVKVICLGSL
ASKLSRKYVKAKKSIDKDEEFAGSSPQSSKSTVVKSLIDNSSEIEDEDQI
LENSSQKENVFKCDSLKEECIPEHMVRGSKIMEAESQKATRECEKSALTP
GFSDNAIMLFSPDFTLRNTLKSTSEDSLVKQECIKEVVEKREACHCEEVK
MTVASEAKIQLSDSEAKSHSSADDASAWSNIQEAPLQDDSCLNNDIPHSI
PLEQGSSCNGPGQTTGHPYYLRSFLVVLKTVLENEDDMLLFDEQEKGIVT
KFYQLSATGQKLYVRLFQRKLSWIKMTKLEYEEIALDLTPVIEELTNAGF
LQTESELQELSEVLELLSAPELKSLAKTFHLVNPNGQKQQLVDAFLKLAK
QRSVCTWGKNKPGIGAVILKRAKALAGQSVRICKGPRAVFSRILLLFSLT
DSMEDEDAACGGQGQLSTVLLVNLGRMEFPSYTINRKTHIFQDRDDLIRY
AAATHMLSDISSAMANGNWEEAKELAQCAKRDWNRLKNHPSLRCHEDLPL
FLRCFTVGWIYTRILSRFVEILQRLHMYEEAVRELESLLSQRIYCPDSRG
RWWDRLALNLHQHLKRLEPTIKCITEGLADPEVRTGHRLSLYQRAVRLRE
SPSCKKFKHLFQQLPEMAVQDVKHVTITGRLCPQRGMCKSVFVMEAGEAA
DPTTVLCSVEELALAHYRRSGFDQGIHGEGSTFSTLYGLLLWDIIFMDGI
PDVFRNACQAFPLDLCTDSFFTSRRPALEARLQLIHDAPEESLRAWVAAT
WHEQEGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRHLAADFRHCR
GGLPDLVVWNSQSRHFKLVEVKGPNDRLSHKQMIWLAELQKLGAEVEVCH
VVAVGAKSQSLS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24248
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Has Puro selection, not hygro. non-Origene; All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.