cDNA Clone FAN1

• Synonyms: MTMR15
• Plasmid: pcDNA5-FRT/TO-mCherry FAN1 (MTMR15)
• Parent Plasmid: pcDNA5 FRT/TO mCherry
• Expressed: mCherry FAN1
• Genbank: NM_014967.4 GI:226246522 with t3015c (silent)
• Tag: mCherry
• Species: Human

Sequence of Insert

agatctatgatgtcagaagggaaacctcctgacaaaaaaaggcctcgtag aagcttatcaatcagcaagaataagaaaaaagcatctaattctattattt cgtgttttaacaatgcaccacctgctaaacttgcctgccccgtttgcagt aaaatggtgcctagatatgacttaaaccggcaccttgatgaaatgtgtgc taacaatgacttcgttcaagtggatccagggcaggttggcttaataaatt caaatgtgtctatggtagatttaaccagtgttaccttagaagatgtaaca cctaagaagtcaccaccaccaaagacaaatttaacccctggccaaagtga ttcagcaaaaagggaagtaaagcagaagatcagtccctactttaaaagta atgatgtggtgtgcaaaaatcaagatgagctgagaaatcgtagtgtgaaa gtcatttgtttgggaagcctagcatctaaattgtccagaaaatacgtaaa ggctaaaaaatcaatagataaggatgaagaatttgccggttctagtccac agagttccaaatccacagttgttaagagcctgattgataactcttcagaa attgaggacgaggatcaaattttggagaacagttctcaaaaagaaaacgt gtttaaatgtgattctctaaaggaagagtgcattcctgaacatatggtaa gaggaagtaaaataatggaagccgaaagccaaaaggctacccgggaatgt gagaaatcagccctcacccctggattctcagataatgcgatcatgttatt ctcaccagatttcactcttaggaatacattaaagtctacttcagaagaca gtcttgtaaagcaagagtgtatcaaagaagtggttgaaaaacgtgaggca tgtcattgtgaagaagtaaaaatgactgttgcttcagaagctaaaataca gctgtcagattcagaggcaaaatctcatagttctgcagatgatgcttctg catggagtaacatccaagaggctcctctgcaggatgacagttgcttaaac aatgatatccctcacagcattcctttggagcaggggtcaagctgcaatgg tcctggtcaaacaaccggtcatccttactaccttcggagtttccttgtgg tgctgaaaaccgtacttgagaatgaagatgatatgttgctctttgatgag caggagaagggaattgtaactaaattttatcagttatcagctactggtca gaagttatatgtaaggctctttcaacgtaaattaagctggattaagatga ccaaattagagtatgaagagattgccttagacttaacacctgtgattgaa gaattgacgaatgcaggctttctacagacagaatctgagttgcaagaact ctctgaagtgcttgaactcctttctgctcctgaactaaaatccctagcca agaccttccacttggtgaatcccaatggacagaaacagcagctggtggac gcctttctcaaattggccaaacagcgttcagtctgcacttggggcaagaa taagcctggaattggtgcagtgattttaaaaagagccaaagccttggctg gacagtcagtacgaatctgtaaaggccccagggctgtgttttcccgcatc ttgctactgttttcgttgaccgactcaatggaagatgaagacgccgcttg tggaggtcagggacagctttcaacagtcctgttggtcaacctcggccgaa tggagtttcctagttacaccatcaatcggaaaacccacatcttccaagac agagatgatcttatcagatatgcagcagccacgcacatgctgagtgacat ttcttccgcaatggccaatgggaactgggaagaagctaaggagctcgctc agtgtgcaaaaagggattggaacagactgaaaaaccacccttctctgaga tgccacgaagatttaccactcttcctgcggtgtttcactgttgggtggat ttatacaaggattttgtctcggtttgtggaaatactgcagagacttcaca tgtatgaggaagccgtcagagaacttgaaagccttttgtctcagagaatt tattgtcctgacagcagaggccgatggtgggatcgactggcccttaattt acaccagcacttgaagcgcctggaaccgactatcaagtgcatcacagagg ggctggcggatccggaagtcagaacgggacaccgcctttcactgtatcag cgagccgtgcgcctgcgagagtctccgagctgtaaaaagttcaagcacct cttccagcagctcccagaaatggctgtgcaagatgtgaaacacgtgacca tcacaggcaggctgtgcccacagcgtgggatgtgcaagtctgtgtttgtg atggaggccggggaggccgctgaccccaccacggtcctgtgctctgtgga ggagctggcactggcccattacagacgcagcggttttgaccaggggattc atggcgaagggtccaccttcagcaccctgtatggcctcctcctgtgggac atcatcttcatggatgggattccggatgtcttcagaaacgcctgtcaggc attccccctggacttgtgcacagacagcttcttcacaagcagacgcccag cccttgaggccaggctgcagctgattcatgatgcccccgaggagagcctg cgggcctgggtggcagccacgtggcatgagcaggaaggcagagtggcttc ccttgtcagctgggatcgcttcacgtctcttcagcaagctcaggatcttg tctcctgcctggggggccctgtgctcagtggtgtgtgcaggcacctggct gctgactttcgacactgtcgagggggcctccccgacctggtggtgtggaa ctcccagagccgtcactttaagctggtggaagttaaaggccccaatgatc gtctttcacataagcagatgatctggctggctgaactgcagaagctgggg gctgaagtagaagtctgccacgtggttgcagttggagctaagagccaaag ccttagctaagcggccgc

Amino Acid Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWER
VMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEA
SSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV
NIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKSGLGSMMSEGKPPD
KKRPRRSLSISKNKKKASNSIISCFNNAPPAKLACPVCSKMVPRYDLNRH
LDEMCANNDFVQVDPGQVGLINSNVSMVDLTSVTLEDVTPKKSPPPKTNL
TPGQSDSAKREVKQKISPYFKSNDVVCKNQDELRNRSVKVICLGSLASKL
SRKYVKAKKSIDKDEEFAGSSPQSSKSTVVKSLIDNSSEIEDEDQILENS
SQKENVFKCDSLKEECIPEHMVRGSKIMEAESQKATRECEKSALTPGFSD
NAIMLFSPDFTLRNTLKSTSEDSLVKQECIKEVVEKREACHCEEVKMTVA
SEAKIQLSDSEAKSHSSADDASAWSNIQEAPLQDDSCLNNDIPHSIPLEQ
GSSCNGPGQTTGHPYYLRSFLVVLKTVLENEDDMLLFDEQEKGIVTKFYQ
LSATGQKLYVRLFQRKLSWIKMTKLEYEEIALDLTPVIEELTNAGFLQTE
SELQELSEVLELLSAPELKSLAKTFHLVNPNGQKQQLVDAFLKLAKQRSV
CTWGKNKPGIGAVILKRAKALAGQSVRICKGPRAVFSRILLLFSLTDSME
DEDAACGGQGQLSTVLLVNLGRMEFPSYTINRKTHIFQDRDDLIRYAAAT
HMLSDISSAMANGNWEEAKELAQCAKRDWNRLKNHPSLRCHEDLPLFLRC
FTVGWIYTRILSRFVEILQRLHMYEEAVRELESLLSQRIYCPDSRGRWWD
RLALNLHQHLKRLEPTIKCITEGLADPEVRTGHRLSLYQRAVRLRESPSC
KKFKHLFQQLPEMAVQDVKHVTITGRLCPQRGMCKSVFVMEAGEAADPTT
VLCSVEELALAHYRRSGFDQGIHGEGSTFSTLYGLLLWDIIFMDGIPDVF
RNACQAFPLDLCTDSFFTSRRPALEARLQLIHDAPEESLRAWVAATWHEQ
EGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRHLAADFRHCRGGLP
DLVVWNSQSRHFKLVEVKGPNDRLSHKQMIWLAELQKLGAEVEVCHVVAV
GAKSQSLS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5-FRT/TO-mCherry FAN1 (MTMR15)73.87 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU49002
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.
• Low Temp: No