cDNA Clone FAN1

• Synonyms: KIAA1018; KMIN; MTMR15; RP11-540B6.6
• Plasmid: pBabe.puro.FLAG.DU FAN1 (MTMR15)
• Parent Plasmid: pBabeD puro FLAG 1
• Expressed: FLAG-FAN1
• Genbank: NM_014967.4 GI:226246522 with t3015c (silent)
• Tag: FLAG
• Species: Human

Sequence of Insert

agatctatgatgtcagaagggaaacctcctgacaaaaaaaggcctcgtag
aagcttatcaatcagcaagaataagaaaaaagcatctaattctattattt
cgtgttttaacaatgcaccacctgctaaacttgcctgccccgtttgcagt
aaaatggtgcctagatatgacttaaaccggcaccttgatgaaatgtgtgc
taacaatgacttcgttcaagtggatccagggcaggttggcttaataaatt
caaatgtgtctatggtagatttaaccagtgttaccttagaagatgtaaca
cctaagaagtcaccaccaccaaagacaaatttaacccctggccaaagtga
ttcagcaaaaagggaagtaaagcagaagatcagtccctactttaaaagta
atgatgtggtgtgcaaaaatcaagatgagctgagaaatcgtagtgtgaaa
gtcatttgtttgggaagcctagcatctaaattgtccagaaaatacgtaaa
ggctaaaaaatcaatagataaggatgaagaatttgccggttctagtccac
agagttccaaatccacagttgttaagagcctgattgataactcttcagaa
attgaggacgaggatcaaattttggagaacagttctcaaaaagaaaacgt
gtttaaatgtgattctctaaaggaagagtgcattcctgaacatatggtaa
gaggaagtaaaataatggaagccgaaagccaaaaggctacccgggaatgt
gagaaatcagccctcacccctggattctcagataatgcgatcatgttatt
ctcaccagatttcactcttaggaatacattaaagtctacttcagaagaca
gtcttgtaaagcaagagtgtatcaaagaagtggttgaaaaacgtgaggca
tgtcattgtgaagaagtaaaaatgactgttgcttcagaagctaaaataca
gctgtcagattcagaggcaaaatctcatagttctgcagatgatgcttctg
catggagtaacatccaagaggctcctctgcaggatgacagttgcttaaac
aatgatatccctcacagcattcctttggagcaggggtcaagctgcaatgg
tcctggtcaaacaaccggtcatccttactaccttcggagtttccttgtgg
tgctgaaaaccgtacttgagaatgaagatgatatgttgctctttgatgag
caggagaagggaattgtaactaaattttatcagttatcagctactggtca
gaagttatatgtaaggctctttcaacgtaaattaagctggattaagatga
ccaaattagagtatgaagagattgccttagacttaacacctgtgattgaa
gaattgacgaatgcaggctttctacagacagaatctgagttgcaagaact
ctctgaagtgcttgaactcctttctgctcctgaactaaaatccctagcca
agaccttccacttggtgaatcccaatggacagaaacagcagctggtggac
gcctttctcaaattggccaaacagcgttcagtctgcacttggggcaagaa
taagcctggaattggtgcagtgattttaaaaagagccaaagccttggctg
gacagtcagtacgaatctgtaaaggccccagggctgtgttttcccgcatc
ttgctactgttttcgttgaccgactcaatggaagatgaagacgccgcttg
tggaggtcagggacagctttcaacagtcctgttggtcaacctcggccgaa
tggagtttcctagttacaccatcaatcggaaaacccacatcttccaagac
agagatgatcttatcagatatgcagcagccacgcacatgctgagtgacat
ttcttccgcaatggccaatgggaactgggaagaagctaaggagctcgctc
agtgtgcaaaaagggattggaacagactgaaaaaccacccttctctgaga
tgccacgaagatttaccactcttcctgcggtgtttcactgttgggtggat
ttatacaaggattttgtctcggtttgtggaaatactgcagagacttcaca
tgtatgaggaagccgtcagagaacttgaaagccttttgtctcagagaatt
tattgtcctgacagcagaggccgatggtgggatcgactggcccttaattt
acaccagcacttgaagcgcctggaaccgactatcaagtgcatcacagagg
ggctggcggatccggaagtcagaacgggacaccgcctttcactgtatcag
cgagccgtgcgcctgcgagagtctccgagctgtaaaaagttcaagcacct
cttccagcagctcccagaaatggctgtgcaagatgtgaaacacgtgacca
tcacaggcaggctgtgcccacagcgtgggatgtgcaagtctgtgtttgtg
atggaggccggggaggccgctgaccccaccacggtcctgtgctctgtgga
ggagctggcactggcccattacagacgcagcggttttgaccaggggattc
atggcgaagggtccaccttcagcaccctgtatggcctcctcctgtgggac
atcatcttcatggatgggattccggatgtcttcagaaacgcctgtcaggc
attccccctggacttgtgcacagacagcttcttcacaagcagacgcccag
cccttgaggccaggctgcagctgattcatgatgcccccgaggagagcctg
cgggcctgggtggcagccacgtggcatgagcaggaaggcagagtggcttc
ccttgtcagctgggatcgcttcacgtctcttcagcaagctcaggatcttg
tctcctgcctggggggccctgtgctcagtggtgtgtgcaggcacctggct
gctgactttcgacactgtcgagggggcctccccgacctggtggtgtggaa
ctcccagagccgtcactttaagctggtggaagttaaaggccccaatgatc
gtctttcacataagcagatgatctggctggctgaactgcagaagctgggg
gctgaagtagaagtctgccacgtggttgcagttggagctaagagccaaag
ccttagctaagcggccgc

Amino Acid Sequence

MDYKDDDDKGSMMSEGKPPDKKRPRRSLSISKNKKKASNSIISCFNNAPP
AKLACPVCSKMVPRYDLNRHLDEMCANNDFVQVDPGQVGLINSNVSMVDL
TSVTLEDVTPKKSPPPKTNLTPGQSDSAKREVKQKISPYFKSNDVVCKNQ
DELRNRSVKVICLGSLASKLSRKYVKAKKSIDKDEEFAGSSPQSSKSTVV
KSLIDNSSEIEDEDQILENSSQKENVFKCDSLKEECIPEHMVRGSKIMEA
ESQKATRECEKSALTPGFSDNAIMLFSPDFTLRNTLKSTSEDSLVKQECI
KEVVEKREACHCEEVKMTVASEAKIQLSDSEAKSHSSADDASAWSNIQEA
PLQDDSCLNNDIPHSIPLEQGSSCNGPGQTTGHPYYLRSFLVVLKTVLEN
EDDMLLFDEQEKGIVTKFYQLSATGQKLYVRLFQRKLSWIKMTKLEYEEI
ALDLTPVIEELTNAGFLQTESELQELSEVLELLSAPELKSLAKTFHLVNP
NGQKQQLVDAFLKLAKQRSVCTWGKNKPGIGAVILKRAKALAGQSVRICK
GPRAVFSRILLLFSLTDSMEDEDAACGGQGQLSTVLLVNLGRMEFPSYTI
NRKTHIFQDRDDLIRYAAATHMLSDISSAMANGNWEEAKELAQCAKRDWN
RLKNHPSLRCHEDLPLFLRCFTVGWIYTRILSRFVEILQRLHMYEEAVRE
LESLLSQRIYCPDSRGRWWDRLALNLHQHLKRLEPTIKCITEGLADPEVR
TGHRLSLYQRAVRLRESPSCKKFKHLFQQLPEMAVQDVKHVTITGRLCPQ
RGMCKSVFVMEAGEAADPTTVLCSVEELALAHYRRSGFDQGIHGEGSTFS
TLYGLLLWDIIFMDGIPDVFRNACQAFPLDLCTDSFFTSRRPALEARLQL
IHDAPEESLRAWVAATWHEQEGRVASLVSWDRFTSLQQAQDLVSCLGGPV
LSGVCRHLAADFRHCRGGLPDLVVWNSQSRHFKLVEVKGPNDRLSHKQMI
WLAELQKLGAEVEVCHVVAVGAKSQSLS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49026
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.