cDNA Clone ERCC5

• Synonyms: COFS3; ERCM2; UVDR; XPG; XPGC
• Plasmid: pEX ERCC5-6His
• Parent Plasmid: pEX C-GST
• Expressed: ERCC5-6His
• Genbank: NM_000123.3 GI:324715039 with silent change t138c
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCGCCACCATGGGGGTCCAGGGGCTCTGGAAGCTGCTGGAGTGCTCCGGGCGGCAGGTCAGCCCCGAAGCGCTGGAAGGGAAGATCCTGGCTGTTGATATTAGCATTTGGTTAAACCAAGCACTTAAAGGAGTCCGGGATCGCCACGGGAACTCAATAGAAAATCCTCATCTTCTCACTTTGTTTCATCGGCTCTGCAAACTCTTATTTTTTCGAATTCGTCCTATTTTTGTGTTTGATGGGGATGCTCCACTATTGAAGAAACAGACTTTGGTGAAGAGAAGGCAGAGAAAGGACTTAGCGTCCAGTGACTCCAGGAAAACGACAGAGAAGCTTCTGAAAACATTTTTGAAAAGACAAGCCATCAAAACTGCCTTCAGAAGCAAAAGAGATGAAGCACTACCCAGTCTTACCCAAGTTCGAAGAGAAAACGACCTCTATGTTTTGCCTCCTTTACAAGAGGAAGAAAAACACAGTTCAGAAGAGGAAGATGAAAAAGAATGGCAAGAAAGAATGAATCAAAAACAAGCATTACAGGAAGAGTTCTTTCATAATCCTCAAGCGATAGATATTGAGTCTGAGGACTTCAGCAGCCTGCCCCCTGAAGTAAAGCATGAAATCTTGACTGATATGAAAGAGTTCACCAAGCGCAGAAGAACATTATTTGAAGCAATGCCAGAGGAGTCTGATGACTTTTCACAGTACCAACTCAAAGGCTTGCTTAAAAAGAACTATCTGAACCAGCATATAGAACATGTCCAAAAGGAAATGAATCAGCAACATTCAGGACACATCCGAAGGCAGTATGAAGATGAAGGGGGCTTTCTGAAGGAGGTAGAGTCAAGGAGAGTGGTCTCTGAAGACACTTCACATTACATCTTGATAAAAGGTATTCAAGCTAAGACAGTTGCAGAAGTGGATTCAGAGTCTCTTCCTTCTTCCAGCAAAATGCACGGCATGTCTTTTGACGTGAAGTCATCTCCATGTGAAAAACTGAAGACAGAGAAAGAGCCTGATGCTACCCCTCCTTCTCCAAGAACTTTACTAGCTATGCAAGCTGCCCTGCTGGGAAGTAGCTCAGAAGAGGAGCTGGAGAGTGAAAATCGAAGGCAGGCCCGTGGGAGGAACGCACCTGCTGCTGTAGACGAAGGCTCCATATCACCCCGGACTCTTTCAGCCATTAAGAGAGCTCTTGACGATGACGAAGATGTAAAAGTGTGTGCTGGGGATGATGTGCAGACGGGAGGGCCAGGAGCAGAAGAAATGCGTATAAACAGCTCCACCGAGAACAGTGATGAAGGACTTAAAGTGAGAGATGGAAAAGGAATACCGTTTACTGCAACACTTGCGTCATCTAGTGTGAACTCTGCAGAGGAGCACGTAGCCAGCACTAATGAGGGGAGAGAGCCCACAGACTCAGTTCCAAAAGAACAAATGTCACTTGTTCACGTGGGGACTGAAGCCTTTCCGATAAGTGATGAGTCTATGATTAAGGACAGAAAAGATCGGCTGCCTCTGGAGAGTGCAGTGGTTAGACATAGTGACGCACCTGGGCTCCCGAATGGAAGGGAACTGACACCGGCATCTCCAACTTGTACAAATTCTGTGTCAAAGAATGAAACACATGCTGAAGTGCTTGAGCAGCAGAACGAACTTTGCCCATATGAGAGTAAATTCGATTCTTCTCTTCTTTCAAGTGATGATGAAACAAAATGTAAACCGAATTCTGCTTCTGAAGTCATTGGCCCTGTCAGTTTGCAAGAAACAAGTAGCATAGTAAGTGTCCCTTCAGAGGCAGTAGATAATGTGGAAAATGTGGTGTCATTTAATGCTAAAGAGCATGAGAATTTTCTGGAAACCATCCAAGAACAGCAGACCACTGAATCTGCAGGCCAGGATTTAATTTCCATTCCAAAGGCCGTGGAACCAATGGAAATTGACTCGGAAGAAAGTGAATCTGATGGAAGTTTCATTGAAGTGCAAAGTGTGATTAGTGATGAGGAACTTCAAGCAGAATTCCCTGAAACTTCCAAACCTCCCTCAGAACAAGGCGAAGAGGAACTGGTAGGAACTAGGGAGGGAGAAGCCCCTGCTGAGTCCGAGAGCCTCCTGAGGGACAACTCTGAGAGGGACGACGTGGATGGTGAGCCACAGGAAGCTGAGAAAGATGCGGAAGATTCGCTCCATGAATGGCAAGATATTAATTTGGAGGAGTTGGAAACTCTGGAGAGCAACCTCTTAGCACAGCAGAATTCACTGAAAGCTCAAAAACAGCAGCAAGAACGGATCGCTGCTACTGTCACCGGACAGATGTTCCTGGAAAGCCAGGAACTCCTGCGCCTGTTCGGCATTCCCTACATCCAGGCTCCCATGGAAGCAGAGGCGCAGTGCGCCATCCTGGACCTGACTGATCAGACTTCCGGAACCATCACTGATGACAGTGATATCTGGCTGTTTGGAGCGCGGCATGTCTATAGAAACTTTTTTAATAAAAACAAGTTTGTAGAATATTATCAATATGTGGACTTTCACAATCAATTGGGATTGGACCGGAATAAGTTAATAAATTTGGCTTATTTGCTTGGAAGTGATTATACCGAAGGAATACCAACTGTGGGTTGTGTAACCGCCATGGAAATTCTCAATGAATTCCCTGGGCATGGCCTGGAACCTCTCCTAAAATTCTCAGAATGGTGGCATGAAGCTCAAAAAAATCCAAAGATAAGACCTAATCCTCATGACACCAAAGTGAAAAAAAAATTACGGACATTGCAACTCACCCCTGGCTTTCCTAACCCAGCTGTTGCCGAGGCCTACCTCAAACCCGTGGTGGATGACTCGAAGGGATCCTTTCTGTGGGGGAAACCTGATCTCGACAAAATTAGAGAATTTTGTCAGCGGTATTTCGGCTGGAACAGAACGAAGACAGATGAATCTCTGTTTCCTGTATTAAAGCAACTCGATGCCCAGCAGACACAGCTCCGAATTGATTCCTTCTTTAGATTAGCACAACAGGAGAAAGAAGATGCTAAACGTATTAAGAGCCAGAGACTAAACAGAGCTGTGACATGTATGCTAAGGAAAGAGAAAGAAGCAGCAGCCAGCGAAATAGAAGCAGTTTCTGTTGCCATGGAGAAAGAATTTGAGCTACTTGATAAGGCAAAACGAAAAACCCAGAAGAGAGGCATAACAAATACCTTAGAAGAGTCATCAAGCCTGAAAAGAAAGAGGCTTTCAGATTCTAAACGAAAGAATACATGCGGTGGATTTTTGGGGGAGACCTGCCTCTCAGAATCATCTGATGGATCTTCAAGTGAAGATGCTGAAAGTTCATCTTTAATGAATGTACAAAGGAGAACAGCTGCGAAAGAGCCAAAAACCAGTGCTTCAGATTCGCAGAACTCAGTGAAGGAAGCTCCCGTGAAGAATGGAGGTGCGACCACCAGCAGCTCTAGTGATAGTGATGACGATGGAGGGAAAGAGAAGATGGTCCTCGTGACCGCCAGATCTGTGTTTGGGAAGAAAAGAAGGAAACTAAGACGTGCGAGGGGAAGAAAAAGGAAAACCCACCATCACCATCACCATTAAGCGGCCGC

Amino Acid Sequence

MGVQGLWKLLECSGRQVSPEALEGKILAVDISIWLNQALKGVRDRHGNSI
ENPHLLTLFHRLCKLLFFRIRPIFVFDGDAPLLKKQTLVKRRQRKDLASS
DSRKTTEKLLKTFLKRQAIKTAFRSKRDEALPSLTQVRRENDLYVLPPLQ
EEEKHSSEEEDEKEWQERMNQKQALQEEFFHNPQAIDIESEDFSSLPPEV
KHEILTDMKEFTKRRRTLFEAMPEESDDFSQYQLKGLLKKNYLNQHIEHV
QKEMNQQHSGHIRRQYEDEGGFLKEVESRRVVSEDTSHYILIKGIQAKTV
AEVDSESLPSSSKMHGMSFDVKSSPCEKLKTEKEPDATPPSPRTLLAMQA
ALLGSSSEEELESENRRQARGRNAPAAVDEGSISPRTLSAIKRALDDDED
VKVCAGDDVQTGGPGAEEMRINSSTENSDEGLKVRDGKGIPFTATLASSS
VNSAEEHVASTNEGREPTDSVPKEQMSLVHVGTEAFPISDESMIKDRKDR
LPLESAVVRHSDAPGLPNGRELTPASPTCTNSVSKNETHAEVLEQQNELC
PYESKFDSSLLSSDDETKCKPNSASEVIGPVSLQETSSIVSVPSEAVDNV
ENVVSFNAKEHENFLETIQEQQTTESAGQDLISIPKAVEPMEIDSEESES
DGSFIEVQSVISDEELQAEFPETSKPPSEQGEEELVGTREGEAPAESESL
LRDNSERDDVDGEPQEAEKDAEDSLHEWQDINLEELETLESNLLAQQNSL
KAQKQQQERIAATVTGQMFLESQELLRLFGIPYIQAPMEAEAQCAILDLT
DQTSGTITDDSDIWLFGARHVYRNFFNKNKFVEYYQYVDFHNQLGLDRNK
LINLAYLLGSDYTEGIPTVGCVTAMEILNEFPGHGLEPLLKFSEWWHEAQ
KNPKIRPNPHDTKVKKKLRTLQLTPGFPNPAVAEAYLKPVVDDSKGSFLW
GKPDLDKIREFCQRYFGWNRTKTDESLFPVLKQLDAQQTQLRIDSFFRLA
QQEKEDAKRIKSQRLNRAVTCMLRKEKEAAASEIEAVSVAMEKEFELLDK
AKRKTQKRGITNTLEESSSLKRKRLSDSKRKNTCGGFLGETCLSESSDGS
SSEDAESSSLMNVQRRTAAKEPKTSASDSQNSVKEAPVKNGGATTSSSSD
SDDDGGKEKMVLVTARSVFGKKRRKLRRARGRKRKTHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU49052
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee