cDNA Clone MAP4K1

• Synonyms: HPK1
• Plasmid: pET15 6P MAP4K1
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6HIS-3C-MAP4K1
• Genbank: NM_001042600.1
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCCATGGACGTCGTGGACCCTGACATTTTCAATAGAGACCCCCGGGACCACTATGACCTGCTACAGCGGCTGGGTGGCGGCACGTATGGGGAAGTCTTTAAGGCTCGAGACAAGGTGTCAGGGGACCTGGTGGCACTGAAGATGGTGAAGATGGAGCCTGATGATGATGTCTCCACCCTTCAGAAGGAAATCCTCATATTGAAAACTTGCCGGCACGCCAACATCGTGGCCTACCATGGGAGTTATCTCTGGTTGCAGAAACTCTGGATCTGCATGGAATTCTGTGGGGCTGGTTCTCTCCAGGACATCTACCAAGTGACAGGCTCCCTGTCAGAGCTCCAGATTAGCTATGTCTGCCGGGAAGTGCTCCAGGGACTGGCCTATTTGCACTCACAGAAGAAGATACACAGGGACATCAAGGGAGCTAACATCCTCATCAATGATGCTGGGGAGGTCAGATTGGCTGACTTTGGCATCTCGGCCCAGATTGGGGCTACACTGGCCAGACGCCTCTCTTTCATTGGGACACCCTACTGGATGGCTCCGGAAGTGGCAGCTGTGGCCCTGAAGGGAGGATACAATGAGCTGTGTGACATCTGGTCCCTGGGCATCACGGCCATCGAACTGGCCGAGCTACAGCCACCGCTCTTTGATGTGCACCCTCTCAGAGTTCTCTTCCTCATGACCAAGAGTGGCTACCAGCCTCCCCGACTGAAGGAAAAAGGCAAATGGTCGGCTGCCTTCCACAACTTCATCAAAGTCACTCTGACTAAGAGTCCCAAGAAACGACCCAGCGCCACCAAGATGCTCAGTCATCAACTGGTATCCCAGCCTGGGCTGAATCGAGGCCTGATCCTGGATCTTCTTGACAAACTGAAGAATCCCGGGAAAGGACCCTCCATTGGGGACATTGAGGATGAGGAGCCCGAGCTACCCCCTGCTATCCCTCGGCGGATCAGATCCACCCACCGCTCCAGCTCTCTGGGGATCCCAGATGCAGACTGCTGTCGGCGGCACATGGAGTTCAGGAAGCTCCGAGGAATGGAGACCAGACCCCCAGCCAACACCGCTCGCCTACAGCCTCCTCGAGACCTCAGGAGCAGCAGCCCCAGGAAGCAACTGTCAGAGTCGTCTGACGATGACTATGACGACGTGGACATCCCCACCCCTGCAGAGGACACACCTCCTCCACTTCCCCCCAAGCCCAAGTTCCGTTCTCCATCAGACGAGGGTCCTGGGAGCATGGGGGATGATGGGCAGCTGAGCCCGGGGGTGCTGGTCCGGTGTGCCAGTGGGCCCCCACCAAACAGCCCCCGTCCTGGGCCTCCCCCATCCACCAGCAGCCCCCACCTCACCGCCCATTCAGAACCCTCACTCTGGAACCCACCCTCCCGGGAGCTTGACAAGCCCCCACTTCTGCCCCCCAAGAAGGAAAAGATGAAGAGAAAGGGATGTGCCCTTCTCGTAAAGTTGTTCAATGGCTGCCCCCTCCGGATCCACAGCACGGCCGCCTGGACACATCCCTCCACCAAGGACCAGCACCTGCTCCTGGGGGCAGAGGAAGGCATCTTTATCCTGAACCGGAATGACCAGGAGGCCACGCTGGAAATGCTCTTTCCTAGCCGGACTACGTGGGTGTACTCCATCAACAACGTTCTCATGTCTCTCTCAGGAAAGACCCCCCACCTGTATTCTCATAGCATCCTTGGCCTGCTGGAACGGAAAGAGACCAGAGCAGGAAACCCCATCGCTCACATTAGCCCCCACCGCCTACTGGCAAGGAAGAACATGGTTTCCACCAAGATCCAGGACACCAAAGGCTGCCGGGCGTGCTGTGTGGCGGAGGGTGCGAGCTCTGGGGGCCCGTTCCTGTGCGGTGCATTGGAGACGTCCGTTGTCCTGCTTCAGTGGTACCAGCCCATGAACAAATTCCTGCTTGTCCGGCAGGTGCTGTTCCCACTGCCGACGCCTCTGTCCGTGTTCGCGCTGCTGACCGGGCCAGGCTCTGAGCTGCCCGCTGTGTGCATCGGCGTGAGCCCCGGGCGGCCGGGGAAGTCGGTGCTCTTCCACACGGTGCGCTTTGGCGCGCTCTCTTGCTGGCTGGGCGAGATGAGCACCGAGCACAGGGGGCCCGTGCAGGTGACCCAGGTAGAGGAAGATATGGTGATGGTGTTGATGGATGGCTCTGTGAAGCTGGTGACCCCGGAGGGGTCCCCAGTCCGGGGACTTCGCACACCTGAGATCCCCATGACCGAAGCGGTGGAGGCCGTGGCTATGGTTGGAGGTCAGCTTCAGGCCTTCTGGAAGCATGGAGTGCAGGTGTGGGCTCTAGGCTCGGATCAGCTGCTACAGGAGCTGAGAGACCCTACCCTCACTTTCCGTCTGCTTGGCTCCCCCAGGCCTGTAGTGGTGGAGACACGCCCAGTGGATGATCCTACTGCTCCCAGCAACCTCTACATCCAGGAATGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSPEFPGVDSKAAAMDVVDPDIFNRDPRD
HYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILI
LKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQIS
YVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGISAQIGAT
LARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPL
FDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSA
TKMLSHQLVSQPGLNRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIP
RRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLR
SSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMG
DDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRE
LDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLL
LGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSH
SILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEG
ASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTG
PGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQ
VEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQA
FWKHGVQVWALGSDQLLQELRDPTLTFRLLGSPRPVVVETRPVDDPTAPS
NLYIQE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU48070
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains two silent nucleotide changes as compared to the reference sequence; C>T 1569bp and A>G 2127bp.
• Low Temp: No