cDNA Clone Usp38 (Mouse)

• Synonyms: 4631402N15Rik; 4833420O05Rik; AA536967; AU044701; AW544820; mKIAA1891
• Plasmid: pCAGGS puro 3XFLAG mouse USP38
• Parent Plasmid: pCAGGS puro 3FLAG
• Expressed: 3XFLAG-mouse USP38
• Genbank: NM_027554.2 GI:34328300 with differences, see notes section.
• Tag: 3FLAG
• Species: Mouse

Sequence of Insert

GGATCCATGGACAAGATTCTGGAAGGCCTGGTGAGCTCCTCGCACCCGCTGCCCCTCAAGCGGATGATTGTGCGGAAGGTGGTGGAGTTCGCGGAGCATTGGCTCGACGAGGCGCAGTGCGAGGCCATGTTTGACCTGACGACCCGCCTCATCCTGGAGGGCCAGGACCCTTTCCAGCGACAAGTGGGCCACCAGGTGCTGGAGGCCTACGCGCGGTACCACCGGCCGGAGTTCGAGTCCTTCTTCAACAAGACTTTCGTGTTGGGCCTCCTTCAGCAGGGCTACCACTCAGTGGACAGGAAGGACGTCGCCATCCTGGACTACATTCACAACGGCCTGAAGCTGATTATGAGCTGCCCATCGGTGCTGGATCTCTTCAGCCTGCTCCAGGTGGAGGTCCTGCGAATGGTTTGCGAGAGGCCGGAGCCAGTGCTCTGTGCCCGGCTGAGCGACCTCCTGACCGATTTCGTGCAGTGCGTCCCCAAAGGGAAACTGTCAGTTACCTTCTGTCAACAGCTGGTTCGAACGATAGGTCATTTCCAGTGCGTGTCCACTCAGGAGAAGGAACTACGGGAGTATGTCTCCCAGGTAACGAAAGTGAGTACCTTACTACAGAATATTTGGAAGGCTGAGCCCTCCACGCTGCTACCTTCTCTGCAAGAAGTTTTTGCAAGCATCTCTTCCACAGATGCATCCTTTGAGCCCTCGGTTGCCTTGGCAAGCCTGGTGCAGCATATTCCTCTGCAGATGATCACAGTCCTCATCAGGAGCCTAACCACCGACCCAAATGTAAAAGATGCGAGTATGACCCAAGCTCTCTGCAGAATGATTGACTGGCTGTCCTGGCCATTGGCTCAGCATGTAGATACGTGGGTAATTGCTCTCCTGAAAGGACTGGCAGCTGTTCAGAAGTTTACTATTTTGATAGATGTTACTTTGCTGAAAATAGAACTGGTTTTTAACCGACTTTGGTTTCCTCTCGTGAGACCTGGTGCTCTTGCAGTCCTCTCTCATATGCTGCTTAGTTTTCAGCATTCTCCCGAGGCATTCCATGTGATTGTCCCTCATATAGTTAACTTGGTTCACTCCTTCAGAAGTGATGGCCTACCTTCCAGCACAGCCTTCCTAGTACAGTTAACAGAGTTGGTTCACTGTATGATGTACCATTATTCCGGATTCCCAGATCTCTATGAGCCTATTCTGGAGGCAGTAAAGGACTTTCCTAAGCCCAGTGAAGAAAAGATTAAGCTGATTCTCAATCAAAGCGCCTGGACTTCTCAATCCAATGCTTTGGCATCTTGCTTGTCTAGACTTTCTGGAAAATCTGAAACTGGGAAAACTGGTCTTATTAACCTGGGAAACACGTGTTACATGAACAGTGTTTTACAAGCATTGTTTATGGCTACAGAGTTCAGAAGACAAGTATTATCTTTAAATCTAAATGGGTGTAATTCGTTAATGAAAAAATTACAACATCTTTTTGCTTTTTTGGCTCATACACAAAGGGAAGCTTACGCACCTCGGATATTTTTTGAGGCTTCCAGACCTCCGTGGTTTACTCCCAGGTCACAACAGGACTGCTCTGAGTACCTCAGGTTTCTGCTGGACAGACTCCATGAAGAAGAAAAGATCTTGCGAGTTCAGTCCTCACATAAGCCTTCGGAAGGTCTGGACTGTGCTGAAACTTGTTTACAAGAGGTAACCAGTAAGGTAGCAGTGCCCACAGAGTCTCCTGGTACAGGTGACAGTGAGAAGACTCTAATAGAGAAAATGTTTGGAGGGAAGCTACGGACTCACATCTGCTGCCTAAACTGCGGGAGTACCTCACACAAAGTTGAAGCCTTCACAGATCTTTCGCTAGCCTTTTGTCCTAGCCCTTCTGTGGAAGACCTGTCTTTCCAAGATACAGCTTCGTTACCCAGTGCTCAAGATGATGGGCTAATGCAAACCAGTGTCGCTGACCCAGAAGAAGAGCCAGTAGTTTATAATCCAGCAACGGCTGCCTTTGTCTGCGACTCAGTTGTGAATCAAAGAGTATTAGGCAGTCCTCCTGTTGAGTTTCACTGTGCTGAAAGCTCTTCTGTCCCTGAAGAATCTGCAAGGATTCTCATCAGTAAAGATGTACCTCAGAACCCAGGAGGTGAAAGCACAACTTCAGTGACTGACTTACTAAACTACTTTCTGGCTCCAGAGGTTCTTACTGGGGAGAACCAGTACTACTGTGAAAGCTGTGCCTCTCTACAGAACGCAGAGAAAACTATGCAAATCACGGAGGAGCCTGAGTATCTTATCCTTACGCTCCTGAGATTCTCCTACGATCAGAAGTACCATGTGAGAAGAAAAATTTTAGACAATGTGTCACTGCCTCTGGTTTTGGAGTTGCCAGTTAAAAGAACTGCTTCCTTCTCTTCCTTGTCGCAGAGTTGGTCTGTAGATGTTGACTTCACTGATATTAATGAGAACCTACCTAAAAAATTAAAACCTTCTGGGACTGAAGAAGCTTTCTGCCCCAAATTAGTGCCCTACCTATTAAGTTCAGTTGTTGTGCACTCTGGTGTGTCCTCTGAGAGTGGCCATTACTACTCTTATGCCAGAAACATCACAGGTACAGAGTCTTCATACCAGATGTGCCCCCAGTCTGAAAGTCTGGCATTAGCACCCTCCCAGAGTTGTTTACTAGGGGTAGAAAGCCCCAATACAGTCATTGAACAGGACTTGGAAAATAAGGAAATGTCACAAGAGTGGTTTTTATTTAATGACAGCAGGGTGACATTTACTTCATTTCAGTCAGTTCAGAAAATCACAAGTAGGTTTCCAAAAGACACAGCTTATGTGCTTTTGTACAAAAAACAAAGCAGAGCTAATGGCATAGGCAGTGACAATCCAGCCAGTGGTGTCTGGGTCAATGGAGATCCACCCCTACAGAAAGAGCTGATGGATGCCATAACAAAGGACAACAAGCTGTATTTGCAGGAACAAGAATTGAATGCTCGAGCCCGGGCCCTGCAAGCTGCATCTGCTTCCTGTTCGTTCCGACCCAATGGATTTGATGACAATGACCCACCAGGAAGCTGTGGGCCAACTGGTGGGGGCGGTGGAGGAGGCTTCAATACAGTTGGCAGACTCGTATTTTGAGCGGCCGC

Amino Acid Sequence

MDYKDHDGDYKDHDIDYKDDDDKGSMDKILEGLVSSSHPLPLKRMIVRKV
VEFAEHWLDEAQCEAMFDLTTRLILEGQDPFQRQVGHQVLEAYARYHRPE
FESFFNKTFVLGLLQQGYHSVDRKDVAILDYIHNGLKLIMSCPSVLDLFS
LLQVEVLRMVCERPEPVLCARLSDLLTDFVQCVPKGKLSVTFCQQLVRTI
GHFQCVSTQEKELREYVSQVTKVSTLLQNIWKAEPSTLLPSLQEVFASIS
STDASFEPSVALASLVQHIPLQMITVLIRSLTTDPNVKDASMTQALCRMI
DWLSWPLAQHVDTWVIALLKGLAAVQKFTILIDVTLLKIELVFNRLWFPL
VRPGALAVLSHMLLSFQHSPEAFHVIVPHIVNLVHSFRSDGLPSSTAFLV
QLTELVHCMMYHYSGFPDLYEPILEAVKDFPKPSEEKIKLILNQSAWTSQ
SNALASCLSRLSGKSETGKTGLINLGNTCYMNSVLQALFMATEFRRQVLS
LNLNGCNSLMKKLQHLFAFLAHTQREAYAPRIFFEASRPPWFTPRSQQDC
SEYLRFLLDRLHEEEKILRVQSSHKPSEGLDCAETCLQEVTSKVAVPTES
PGTGDSEKTLIEKMFGGKLRTHICCLNCGSTSHKVEAFTDLSLAFCPSPS
VEDLSFQDTASLPSAQDDGLMQTSVADPEEEPVVYNPATAAFVCDSVVNQ
RVLGSPPVEFHCAESSSVPEESARILISKDVPQNPGGESTTSVTDLLNYF
LAPEVLTGENQYYCESCASLQNAEKTMQITEEPEYLILTLLRFSYDQKYH
VRRKILDNVSLPLVLELPVKRTASFSSLSQSWSVDVDFTDINENLPKKLK
PSGTEEAFCPKLVPYLLSSVVVHSGVSSESGHYYSYARNITGTESSYQMC
PQSESLALAPSQSCLLGVESPNTVIEQDLENKEMSQEWFLFNDSRVTFTS
FQSVQKITSRFPKDTAYVLLYKKQSRANGIGSDNPASGVWVNGDPPLQKE
LMDAITKDNKLYLQEQELNARARALQAASASCSFRPNGFDDNDPPGSCGP
TGGGGGGGFNTVGRLVF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49091
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Differences compared with reference sequence:; g354a (silent); t1035c (silent); c1164t (silent); a1329g (silent); g1695a (silent); t1809c (silent); a1992g (silent); a2096g (K699R); a2208g (silent); c2412g (silent); t2532a (silent); a2867g (D956G); c2897t (A966V); ; All coding changes seen in other published sequences e.g. BAC98274.1