cDNA Clone Fancd2 (mouse)

• Synonyms: 2410150O07Rik; AU015151; BB137857; FA-D2; FA4; FACD; FAD; FANCD
• Plasmid: pCAGGS puro 3XFLAG mouse fancd2 S10A
• Parent Plasmid: pCAGGS puro 3FLAG
• Expressed: 3XFLAG mouse fancd2 S10A
• Genbank: NM_001033244.3 GI:282721082 with silent change t4203c to kill internal BamHI
• Tag: 3FLAG
• Species: Mouse

Sequence of Insert

GGATCCATGATTTCCAAAAGACGTCGGCTAGATGCCGAGGATAAAGAAAACCTGACAGAAGATGCCTCCAAAACCATGCCCCTTTCCAAGCTGGCAAAGAAGTCTCACAATTCTCATGAAGTTGAAGAAAATGGCAGTGTCTTTGTAAAGCTTCTTAAGGCTTCAGGACTCACTCTTAAAACTGGAGAGAACCAAAATCAGCTAGGTGTGGATCAGGTAATCTTCCAAAGGAAGCTCTTTCAGGCCTTGAGGAAGCATCCTGCTTATCCCAAAGTAATAGAAGAGTTTGTTAATGGCCTGGAGTCCTACACTGAGGACAGTGAGAGTCTCAGGAACTGCCTGCTGTCTTGTGAGCGCCTGCAGGATGAGGAAGCCAGCATGGGCACATTTTACTCCAAGAGTCTGATCAAGCTACTTCTGGGGATTGACATTTTACAGCCTGCCATTATCAAAATGTTATTTGAAAAAGTGCCTCAGTTTCTTTTTGAAAGTGAGAACAGAGATGGAATCAACATGGCCAGACTCATTATCAATCAACTAAAATGGCTGGATAGAATTGTGGATGGCAAGGACCTCACGGCCCAGATGATGCAGTTGATCAGTGTTGCTCCCGTGAACTTACAGCATGACTTCATCACGAGCCTTCCTGAAATCCTAGGGGATTCCCAGCATGCTAATGTGGGGAAAGAGCTTGGCGAGCTGCTGGTGCAGAATACTTCCCTGACTGTTCCAATTTTGGATGTCTTTTCCAGTCTCCGACTTGACCCCAACTTCCTGTCCAAGATCCGCCAGTTGGTGATGGGCAAGCTGTCATCTGTCCGTCTAGAGGATTTCCCTGTGATTGTAAAGTTCCTTCTTCATTCTGTAACAGACACCACTTCCCTTGAGGTCATTGCCGAGCTTCGGGAGAACTTGAACGTCCAGCAGTTTATTTTGCCGTCACGAATTCAGGCTTCCCAAAGCAAATTGAAAAGTAAAGGACTAGCAAGCTCTTCAGGAAATCAAGAGAACAGTGATAAAGACTGTATTGTTCTTGTCTTTGATGTAATAAAGTCAGCCATTAGATATGAGAAAACCATTTCAGAGGCCTGGTTTAAGGCAATTGAACGCATTGAGTCCGCGGCTGAACATAAGGCTTTGGACGTGGTCATGCTGCTCATCATCTACAGCACCAGCACGCAGACCAAGAAGGGCGTGGAGAAGCTGCTGAGAAACAAGATTCAGTCAGACTGCATTCAAGAACAGCTGCTTGACAGTGCGTTCTCTACACATTACCTGGTTCTTAAGGATATTTGCCCATCTATTCTTTTGCTGGCTCAGACTTTGTTTCACTCTCAAGACCAGAGGATCATTTTGTTTGGCAGTCTTCTGTACAAATATGCTTTTAAGTTTTTTGATACTTACTGCCAGCAGGAAGTGGTTGGTGCCCTAGTCACCCATGTCTGCAGTGGGACTGAGGCTGAAGTCGACACTGCACTGGATGTCCTCCTGGAGCTGATTGTGCTAAACGCCTCTGCTATGAGGCTCAATGCTGCTTTTGTTAAGGGCATCTTAGATTATTTGGAAAATATGTCCCCTCAGCAAATACGAAAAATCTTCTGTATTCTCAGCACTCTTGCATTTAGCCAACAGCCCGGAACCAGCAACCATATCCAGGACGACATGCACCTGGTGATCCGGAAGCAGCTCTCTAGCACTGTGTTCAAGTACAAGCTCATTGGGATCATTGGTGCAGTCACCATGGCCGGCATCATGGCGGAAGACAGAAGTGTACCATCTAACTCATCCCAGAGGAGCGCCAATGTGAGCAGTGAGCAGCGCACACAGGTGACTTCTTTGCTACAACTAGTTCATTCTTGCACTGAGCACTCTCCTTGGGCCTCTTCTCTGTATTATGATGAATTTGCCAACCTGATCCAAGAAAGGAAGTTGGCTCCAAAAACCTTGGAGTGGGTTGGGCAGACCATCTTCAATGATTTCCAAGATGCCTTTGTGGTAGACTTCTGTGCTGCTCCAGAGGGTGACTTTCCATTTCCTGTGAAAGCGCTCTATGGACTGGAAGAGTACAGCACTCAAGACGGCATTGTCATCAACCTCCTGCCGCTGTTCTATCAGGAATGTGCAAAAGATGCCAGTCGAGCGACATCACAAGAATCGAGCCAGAGATCAATGTCTTCTTTGTGCCTGGCTTCCCATTTCCGGCTGCTGAGACTTTGCGTGGCAAGACAACATGATGGAAACTTGGATGAGATCGATGGTCTCTTAGATTGTCCCCTGTTCCTCCCTGACCTGGAACCTGGAGAGAAACTGGAGTCCATGTCTGCTAAAGACCGTTCGCTTATGTGTTCGCTCACATTCCTAACTTTCAACTGGTTCCGAGAGGTTGTGAATGCCTTCTGCCAACAAACATCTCCTGAGATGAAGGGCAAGGTTCTTAGTCGGCTAAAGGACCTTGTAGAACTTCAGGGAATCCTAGAGAAGTACTTGGCAGTCATCCCAGACTATGTTCCGCCTTTCGCAAGCGTTGACTTGGACACTTTAGATATGATGCCTAGGAGCAGTTCTGCTGTTGCAGCAAAAAACAGAAACAAGGGAAAGACGGGGGGAAAGAAACAAAAAGCTGATAGCAACAAAGCATCCTGTTCGGACACACTTCTAACAGAAGACACTTCAGAGTGTGACATGGCGCCATCTGGGAGAAGCCACGTAGACAAGGAGTCCACAGGGAAGGAAGGAAAGACGTTTGTGTCACTGCAGAATTACCGCGCTTTTTTCCGAGAGCTGGACATTGAGGTCTTCTCTATTCTACATTCTGGACTTGTGACCAAGTTCATCTTAGACACTGAAATGCACACTGAAGCTACAGAGGTCGTACAGCTGGGGCCTGCTGAGCTGCTCTTCTTGCTGGAAGATCTTTCCCAGAAGCTAGAGAATATGCTGACTGCTCCTTTTGCCAAGAGAATCTGCTGCTTTAAGAATAAAGGAAGGCAGAATATTGGCTTCTCACATCTTCATCAGAGATCTGTCCAGGACATTGTGCACTGTGTGGTTCAGCTGCTAACCCCGATGTGTAACCATCTGGAGAACATTCACAACTTCTTTCAGTGCTTAGGTGCTGAGCATCTCAGTGCAGATGACAAGGCGAGAGCGACAGCTCAGGAGCAGCACACCATGGCCTGCTGCTACCAGAAGCTGCTGCAGGTCTTGCACGCGCTCTTTGCGTGGAAGGGATTTACTCACCAATCAAAGCACCGCCTCCTGCACTCAGCCCTTGAGGTCCTCTCGAACCGACTAAAGCAGATGGAACAGGACCAGCCCTTGGAGGAACTGGTCAGCCAGAGCTTCAGTTACTTGCAGAACTTCCACCATAGTGTTCCCAGTTTCCAGTGTGGTCTCTACCTTCTCAGACTTCTGATGGCCCTTCTGGAGAAGTCTGCAGTACCTAACCAGAAGAAAGAAAAACTTGCCTCTCTGGCCAAACAGCTGCTTTGCCGAGCATGGCCTCATGGGGAAAAAGAGAAGAACCCCACTTTTAATGACCACCTGCATGATGTGCTTTACATCTACTTGGAGCACACAGACAATGTTCTGAAGGCCATAGAGGAGATCACTGGTGTTGGTGTCCCAGAACTGGTCAGTGCTCCGAAAGACGCCGCCTCCTCTACATTCCCTACGTTGACCAGGCACACCTTTGTCATATTCTTCCGTGTGATGATGGCTGAACTCGAGAAGACGGTGAAGGGTCTTCAGGCTGGCACAGCAGCAGATTCGCAGCAGGTTCACGAAGAGAAGCTCCTCTATTGGAACATGGCTGTCCGAGATTTCAGCATCCTTCTCAATCTGATGAAAGTATTTGACAGTTATCCTGTTCTGCATGTGTGTTTAAAGTATGGCCGTCGCTTTGTGGAGGCATTTCTGAAGCAATGTATGCCACTCCTCGACTTCAGCTTTAGAAAGCATCGGGAAGATGTTCTGAGCTTGCTGCAAACCCTTCAGTTGAACACGAGGCTACTTCATCACCTTTGTGGACACTCCAAGATTCGCCAGGACACAAGACTCACCAAGCATGTGCCTTTACTCAAAAAGTCACTGGAACTGTTAGTTTGCAGAGTCAAAGCCATGCTTGTCCTCAACAACTGTAGAGAGGCTTTCTGGTTGGGTACTCTCAAAAACCGAGACTTACAGGGTGAAGAAATTATTTCCCAGGACCCCTCTTCCTCAGAGAGCAATGCAGAGGACAGTGAGGATGGCGTGACATCTCACGTCTCCAGGAACAGAGCAACAGAGGATGGGGAAGATGAAGCAAGTGATGAACAGAAGGACCAGGACAGTGATGAAAGTGACGACAGCTCCAGTTAGCGGCCGC

Amino Acid Sequence

MDYKDHDGDYKDHDIDYKDDDDKGSMISKRRRLDAEDKENLTEDASKTMP
LSKLAKKSHNSHEVEENGSVFVKLLKASGLTLKTGENQNQLGVDQVIFQR
KLFQALRKHPAYPKVIEEFVNGLESYTEDSESLRNCLLSCERLQDEEASM
GTFYSKSLIKLLLGIDILQPAIIKMLFEKVPQFLFESENRDGINMARLII
NQLKWLDRIVDGKDLTAQMMQLISVAPVNLQHDFITSLPEILGDSQHANV
GKELGELLVQNTSLTVPILDVFSSLRLDPNFLSKIRQLVMGKLSSVRLED
FPVIVKFLLHSVTDTTSLEVIAELRENLNVQQFILPSRIQASQSKLKSKG
LASSSGNQENSDKDCIVLVFDVIKSAIRYEKTISEAWFKAIERIESAAEH
KALDVVMLLIIYSTSTQTKKGVEKLLRNKIQSDCIQEQLLDSAFSTHYLV
LKDICPSILLLAQTLFHSQDQRIILFGSLLYKYAFKFFDTYCQQEVVGAL
VTHVCSGTEAEVDTALDVLLELIVLNASAMRLNAAFVKGILDYLENMSPQ
QIRKIFCILSTLAFSQQPGTSNHIQDDMHLVIRKQLSSTVFKYKLIGIIG
AVTMAGIMAEDRSVPSNSSQRSANVSSEQRTQVTSLLQLVHSCTEHSPWA
SSLYYDEFANLIQERKLAPKTLEWVGQTIFNDFQDAFVVDFCAAPEGDFP
FPVKALYGLEEYSTQDGIVINLLPLFYQECAKDASRATSQESSQRSMSSL
CLASHFRLLRLCVARQHDGNLDEIDGLLDCPLFLPDLEPGEKLESMSAKD
RSLMCSLTFLTFNWFREVVNAFCQQTSPEMKGKVLSRLKDLVELQGILEK
YLAVIPDYVPPFASVDLDTLDMMPRSSSAVAAKNRNKGKTGGKKQKADSN
KASCSDTLLTEDTSECDMAPSGRSHVDKESTGKEGKTFVSLQNYRAFFRE
LDIEVFSILHSGLVTKFILDTEMHTEATEVVQLGPAELLFLLEDLSQKLE
NMLTAPFAKRICCFKNKGRQNIGFSHLHQRSVQDIVHCVVQLLTPMCNHL
ENIHNFFQCLGAEHLSADDKARATAQEQHTMACCYQKLLQVLHALFAWKG
FTHQSKHRLLHSALEVLSNRLKQMEQDQPLEELVSQSFSYLQNFHHSVPS
FQCGLYLLRLLMALLEKSAVPNQKKEKLASLAKQLLCRAWPHGEKEKNPT
FNDHLHDVLYIYLEHTDNVLKAIEEITGVGVPELVSAPKDAASSTFPTLT
RHTFVIFFRVMMAELEKTVKGLQAGTAADSQQVHEEKLLYWNMAVRDFSI
LLNLMKVFDSYPVLHVCLKYGRRFVEAFLKQCMPLLDFSFRKHREDVLSL
LQTLQLNTRLLHHLCGHSKIRQDTRLTKHVPLLKKSLELLVCRVKAMLVL
NNCREAFWLGTLKNRDLQGEEIISQDPSSSESNAEDSEDGVTSHVSRNRA
TEDGEDEASDEQKDQDSDESDDSSS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49116
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee