cDNA Clone KIAA2022

• Synonyms: MRX98, XPN
• Plasmid: pMK-RQ KIAA2022 Nter GFP donor
• Parent Plasmid: pMK-RQ
• Genbank: ENSG00000050030
• Tag: GFP
• Species: Human

Sequence of Insert

GGTTAGTCTTGTCTTTGCTTTGTGTCCTTCTTAATCAGGCAACCATGATT
AGGTTAAATGCATTACTATTTGGAGGAAGGGATACGTTTGTCTGCTTCCT
TGGATTTCAGTTTTGAATAATATTGTGGCACAGAGGTGATAGAAGGATCT
AGGATGTATGAATGAGTGTGAGTTTCCAAACTGACTTTATGTGGTAGGTA
TAAATTGTGAAACAAAAACAATAGCAGTGATGAAGTCAATCTGGAAACAG
GTAAATCCCAGTGAGCCTTTTAAGACCCCTCTAGCTGAAATAACCTATAA
AACACAAAATAAAACAAATTTAATGACTGATATTATATATTTGAAATGTG
CAGGTTGGACAGCATTTGGACTTGGAATGAAACAATAAGAGGTTATATTC
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT
CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG
GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACC
ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA
CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT
TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC
TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGG
CGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGG
ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAC
GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA
GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC
AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGATGGACAATCAGCAAGATAAAGCTATTGTTGCC
TCAGCCAACGGAGAAAACACTCTGATTAATGGGGTCAAAGAAAATGGTAA
GGGAATTTAATTATAGTATATTAATATCTCTCCTGGTAGTCCTCTGTTAT
TACTATGGATTTTCAGTACCCACAAAGTTGGGACAGGTTGAGTCCAGGAT
GCCTCTGAAATGAATGTTAATTGTAAACTTTATTCCACCAAATATCTAAC
TTGGCTATAAGAGTCTCTAAGAGGCATTGAATCTGACCACTTTGCCCTCC
CAAATCATATCTGCCAGATGAAGATCCTCAAAATTCCTAGTGAATTAGAA
ATATGCTGTGATGTGAGGCTTAGAACTTCTAGAGTGGCCGGGCGCAGTGG
CTCATGCCTGTAATCCCAGTACTTTGGGAGGCCGAGGTGGGCAGATCATT
TGAGGTCAGGAGTTCGAGACCAGACTGGCCAACATGGTGAAACCCCATCT
CTGCCAAAAATATAAAAAATTAGCCGGGTGTGGTGGCA

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKMDNQQDKAIVA
SANGENTLINGVKENDSEDQDVAMKSFAALEAAAPIQPTPVAQKETLMYP
RGLLPLPSKKPCMQSPPSPLGLIEAPEHAANSASVNAISLTSGIAKGLNT
WSLPNECEKAPFAIMEPAGMSALNGDCLMQPSRTCLGCFMESKDAVDPEP
GISLKVGDLNRDYETCAVSDIGIQCINAGENMKYGEQLLSDQLLGFPLHK
SRAGDRRETEKPDIDLEDPAQKSYYEALLLDKCNTEEALLANSNQDWGYF
ETFISESKIELLDLCSKNELSVNLFSEEDVDNYMFDDDESTLGSDVCSLK
IRYESFQDNVRDKTTLLMQEDAQFNFFPSVFTTCPKRESKSGALKQSSDF
SQFKVPDVSIIWGEEDKNLDKKKGKEEGQEDKGVEKKDGKDNGEKPALNK
PCSGTEVEQLKNPKQGHLANSLETSGSFSDDSSFIEISYDAMGEIKDCSR
YMARDTNSGSSSSQQNYGLRAKRKVRYSEDYLYDVDSLEGEKVNERKEWL
PVGSKEEDDDEWCPKKRRKVTRKEPPVIIKYIIINRFKGEKNMLVKLGKV
DASETTVNLSENQLNKYAKLAPLKGFWQKKKKQRNTNTDSIKTPFSQKQS
FEPGSFEVSFLPPARKRKSKLGNRHRIQRIPSIEISASSKQISLCNDQRH
ASNHKEDGGLKGTLKSAPLGAPSCANGSHLNDITGPDSVKVKAQDTEFKG
PERKVLNKIKFKSEARLKSKKVKAAGQESKPIVQMSPLLENQSSKANLKN
EVIPGTSNSSRLSEFHEAKAAKSSTFLPTTCSSEMPLSSANVTTNIPVIP
GGYLQTLLDASDLSNNTSISYFSHHSPEQNEGSLTQTEKSFVPLQPTQDC
VLTSSSDSELQQSSHNFKMESSNYRNVWPNKATSGTQEFMAEVSREIAPT
QSSEFGASQVVSMENNLTPTTYNPICLNSGGSNCNKVLYDSMQDTQLPSD
DSYQLCHFNNGEICFPFQQGPVNMDDGRLFSFDSMAPLSVSSSNYCSLSL
KSCEKDGDDDITDDFLAHCSPKLVIQQSIDEIAPLKESTDLLDISNFTPD
KFRHSSLSEMSPPDTPSLSPQITRCESMKTLGTLKGFQEGVPGPLDSVEK
IKWDCSTLSRQVQMEDGFTLNNHQFQFHMFNDEDSVSLLQKNPCLSTFND
PSGQISTNNKVSKSRKKSSPSKSGAMNQSSSQKNTRKKSLKGNNKGIEKP
PGKNSRQVPKSTKKGKYMAAINGEKMQIGIGRGGSQTNTISSTGKTLAEC
IQHGGPMASMKMPSQKGLSGDWALGKESSPGWSDMSMGTNTNSLLDDDQR
EFQEPSYILSNIASGMADVQRFMMASIEPLWEPMEHHGDPNIFYSPESNS
LKLKTLKILAGTPQESKKKINSGSQGATKNHRSIKGVSKSNGKTAIGDPG
RANMPGYNEDSRSTFFDKKYSNMSTLGNNGPTHKKLYRHKSSSKALRDEK
CKGKHMEREQVHKDESGTASFEKLRDSDYNLLKAETTFWVLPVFEEETRI
FQKDI*

• Vector Type: Other
• Antibiotic: Kan
• DU Number: DU48065
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Donor to target KIAA2022 and insert an in-frame eGFP at the N-terminus. Sequence of tagged product shown as cassette. Contains 3 silent changes to prevent the gRNA binding to the donor.