cDNA Clone M-RIP

• Synonyms: RHOIP3
• Plasmid: pGEX6P-1 M-Rip
• Parent Plasmid: pGEX6P1
• Expressed: GST-M-RIP
• Genbank: NM_015134
• Tag: GST
• Species: Human

Sequence of Insert

gaattcatgtcggcagccaaggagaacccgtgcaggaaattccaggccaacatcttcaacaagagcaagtgtcagaactgcttcaagccccgcgagtcgcatctgctcaacgacgaggacctgacgcaggcaaaacccatttatggcggttggctgctcctggctccagatgggaccgactttgacaacccagtgcaccggtctcggaaatggcagcgacggttcttcatcctttacgagcacggcctcttgcgctacgccctggatgagatgcccacgacccttcctcagggcaccatcaacatg aaccagtgcacagatgtggtggatggggagggccgcacgggccagaagtt ctccctgtgtattctgacgcctgagaaggagcatttcatccgggcggaga ccaaggagatcgtcagtgggtggctggagatgctcatggtctatccccgg accaacaagcagaatcagaagaagaaacggaaagtggagccccccacacc acaggagcctgggcctgccaaggtggctgttaccagcagcagcagcagca gcagcagcagcagcatccccagtgctgagaaagtccccaccaccaagtcc acactctggcaggaagaaatgaggaccaaggaccagccagatggcagcag cctgagtccagctcagagtcccagccagagccagcctcctgctgccagct ccctgcgggaacctgggctagagagcaaagaagaggagagcgccatgagt agcgaccgcatggactgtggccgcaaagtccgggtggagagcggctactt ctctctggagaagaccaaacaggacttgaaggctgaagaacagcagctgc ccccgccgctctcccctcccagccccagcacccccaaccacaggaggtcc caggtgattgaaaagtttgaggccttggacattgagaaggcagagcacat ggagaccaatgcagtggggccctcacaatccagcgacacacgccagggcc gcagcgagaagagggcgttccctaggaagcgggacttcaccaatgaagcc cccccagctcctctcccagacgcctcggcttcccccctgtctccacaccg aagagccaagtcactggacaggaggtccacggagccctccgtgacgcccg acctgctgaatttcaagaaaggctggctgactaagcagtatgaggacggc cagtggaagaaacactggtttgtcctcgccgatcaaagcctgagatacta cagggattcagtggctgaggaggcagccgacttggatggagaaattgact tgtccgcatgttacgatgtcacagagtatccagttcagagaaactatggc ttccagatacatacaaaggagggcgagtttaccctgtcggccatgacatc tgggattcggcggaactggatccagaccatcatgaagcacgtgcacccga ccactgccccggatgtgaccagctcgttgccagaggaaaaaaacaagagc agctgctcttttgagacctgcccgaggcctactgagaagcaagaggcaga gctgggggagccggaccctgagcagaagaggagccgcgcacgggagcgga ggcgagagggccgctccaagacctttgactgggctgagttccgtcccatc cagcaggccctggctcaggagcgggtgggcggcgtggggcctgctgacac ccacgagcccctgcgccctgaggcggagcctggggagctggagcgggagc gtgcacggaggcgggaggagcgccgcaagcgcttcgggatgctcgacgcc acagacgggccaggcactgaggatgcagccctgcgcatggaggtggaccg gagcccagggctgcctatgagcgacctcaaaacgcataacgtccacgtgg agattgagcagcggtggcatcaggtggagaccacacctctccgggaagag aagcaggtgcccatcgcccccgtccacctgtcttctgaagatgggggtga ccggctctccacacacgagctgacctctctgctcgagaaggagctggagc agagccagaaggaggcctcagaccttctggagcagaaccggctcctgcag gaccagctgagggtggccctgggccgggagcagagcgcccgtgagggcta cgtgctgcaggccacgtgcgagcgagggtttgcagcaatggaagaaacgc accagaagaagattgaagatctccagaggcagcaccagcgggagctagag aaacttcgagaagagaaagaccgcctcctagccgaggagacagcggccac catctcagccatcgaagccatgaagaacgcccaccgggaggaaatggagc gggagctggagaagagccagcggtcccagatcagcagcgtcaactcggat gttgaggccctgcggcgccagtacctggaggagctgcagtcggtgcagcg ggaactggaggtcctctcggagcagtactcgcagaagtgcctggagaatg cccatctggcccaggcgctggaggccgagcggcaggccctgcggcagtgc cagcgtgagaaccaggagctcaatgcccacaaccaggagctgaacaaccg cctggctgcagagatcacacggttgcggacgctgctgactggggacggcg gtggggaggccactgggtcaccccttgcacagggcaaggatgcctatgaa ctagaggtcttattgcgggtaaaggaatcggaaatacagtacctgaaaca ggagattagctccctcaaggatgagctgcagacggcactgcgggacaaga agtacgcaagtgacaagtacaaagacatctacacagagctcagcatcgcg aaggctaaggctgactgtgacatcagcaggttgaaggagcagctcaaggc tgcaacggaagcactgggggagaagtcccctgacagtgccacggtgtccg gatatgatataatgaaatctaaaagcaaccctgacttcttgaagaaagac agatcctgtgtcacccggcaactcagaaacatcaggtccaagagtctgaa ggaaggcctgacggtgcaagaacggttgaagctctttgaatccagggact tgaagaaagactaggcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMSAAKENPCRKFQANI
FNKSKCQNCFKPRESHLLNDEDLTQAKPIYGGWLLLAPDGTDFDNPVHRS
RKWQRRFFILYEHGLLRYALDEMPTTLPQGTINMNQCTDVVDGEGRTGQK
FSLCILTPEKEHFIRAETKEIVSGWLEMLMVYPRTNKQNQKKKRKVEPPT
PQEPGPAKVAVTSSSSSSSSSSIPSAEKVPTTKSTLWQEEMRTKDQPDGS
SLSPAQSPSQSQPPAASSLREPGLESKEEESAMSSDRMDCGRKVRVESGY
FSLEKTKQDLKAEEQQLPPPLSPPSPSTPNHRRSQVIEKFEALDIEKAEH
METNAVGPSQSSDTRQGRSEKRAFPRKRDFTNEAPPAPLPDASASPLSPH
RRAKSLDRRSTEPSVTPDLLNFKKGWLTKQYEDGQWKKHWFVLADQSLRY
YRDSVAEEAADLDGEIDLSACYDVTEYPVQRNYGFQIHTKEGEFTLSAMT
SGIRRNWIQTIMKHVHPTTAPDVTSSLPEEKNKSSCSFETCPRPTEKQEA
ELGEPDPEQKRSRARERRREGRSKTFDWAEFRPIQQALAQERVGGVGPAD
THEPLRPEAEPGELERERARRREERRKRFGMLDATDGPGTEDAALRMEVD
RSPGLPMSDLKTHNVHVEIEQRWHQVETTPLREEKQVPIAPVHLSSEDGG
DRLSTHELTSLLEKELEQSQKEASDLLEQNRLLQDQLRVALGREQSAREG
YVLQATCERGFAAMEETHQKKIEDLQRQHQRELEKLREEKDRLLAEETAA
TISAIEAMKNAHREEMERELEKSQRSQISSVNSDVEALRRQYLEELQSVQ
RELEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNAHNQELNN
RLAAEITRLRTLLTGDGGGEATGSPLAQGKDAYELEVLLRVKESEIQYLK
QEISSLKDELQTALRDKKYASDKYKDIYTELSIAKAKADCDISRLKEQLK
AATEALGEKSPDSATVSGYDIMKSKSNPDFLKKDRSCVTRQLRNIRSKSL
KEGLTVQERLKLFESRDLKKD*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P-1 M-Rip182.01 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU46333
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with NM_015134 CDS: c980a (SNP P327Q), delta cag 534-536 (delta S179) - both present in several ESTs and mRNAs.
• Low Temp: No