cDNA Clone WNK1

• Synonyms: HSAN2, HSN2, KDP, PRKWNK1, PSK, p65
• Plasmid: pEBG6P WNK1 tv4
• Parent Plasmid: pEBG6P
• Expressed: GST-WNK1 tv4
• Genbank: NM_018979.3
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGTCTGGCGGCGCCGCAGAGAAGCAGAGCAGCACTCCCGGTTCCCTGTTCCTCTCGCCGCCGGCTCCTGCCCCCAAGAATGGCTCCAGCTCCGATTCCTCCGTGGGGGAGAAACTGGGAGCCGCGGCCGCCGACGCTGTGACCGGCAGGACCGAGGAGTACAGGCGCCGCCGCCACACTATGGACAAGGACAGCCGTGGGGCGGCCGCGACCACTACCACCACTGAGCACCGCTTCTTCCGCCGGAGCGTCATCTGCGACTCCAATGCCACTGCACTGGAGCTTCCCGGCCTTCCTCTTTCCCTGCCCCAGCCCAGCATCCCCGCGGCTGTCCCGCAGAGTGCTCCACCGGAGCCCCACCGGGAAGAGACCGTGACCGCCACCGCCACTTCCCAGGTAGCCCAGCAGCCTCCAGCCGCTGCCGCCCCTGGGGAACAGGCCGTCGCGGGCCCTGCCCCCTCGACTGTCCCCAGCAGTACCAGCAAAGACCGCCCAGTGTCCCAGCCTAGCCTTGTGGGGAGCAAAGAGGAGCCGCCGCCGGCGAGAAGTGGCAGCGGCGGCGGCAGCGCCAAGGAGCCACAGGAGGAACGGAGCCAGCAGCAGGATGATATCGAAGAGCTGGAGACCAAGGCCGTGGGAATGTCTAACGATGGCCGCTTTCTCAAGTTTGACATCGAAATCGGCAGAGGCTCCTTTAAGACGGTCTACAAAGGTCTGGACACTGAAACCACCGTGGAAGTCGCCTGGTGTGAACTGCAGGATCGAAAATTAACAAAGTCTGAGAGGCAGAGATTTAAAGAAGAAGCTGAAATGTTAAAAGGTCTTCAGCATCCCAATATTGTTAGATTTTATGATTCCTGGGAATCCACAGTAAAAGGAAAGAAGTGCATTGTTTTGGTGACTGAACTTATGACGTCTGGAACACTTAAAACGTATCTGAAAAGGTTTAAAGTGATGAAGATCAAAGTTCTAAGAAGCTGGTGCCGTCAGATCCTTAAAGGTCTTCAGTTTCTTCATACTCGAACTCCACCTATCATTCACCGCGATCTTAAATGTGACAACATCTTTATCACCGGCCCTACTGGCTCAGTCAAGATTGGAGACCTCGGTCTGGCAACCCTGAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTCATGGCCCCTGAGATGTATGAGGAGAAATATGATGAATCCGTTGACGTTTATGCTTTTGGGATGTGCATGCTTGAGATGGCTACATCTGAATATCCTTACTCGGAGTGCCAAAATGCTGCGCAGATCTACCGTCGCGTGACCAGTGGGGTGAAGCCAGCCAGTTTTGACAAAGTAGCAATTCCTGAAGTGAAGGAAATTATTGAAGGATGCATACGACAAAACAAAGATGAAAGATATTCCATCAAAGACCTTTTGAACCATGCCTTCTTCCAAGAGGAAACAGGAGTACGGGTAGAATTAGCAGAAGAAGATGATGGAGAAAAAATAGCCATAAAATTATGGCTACGTATTGAAGATATTAAGAAATTAAAGGGAAAATACAAAGATAATGAAGCTATTGAGTTTTCTTTTGATTTAGAGAGAGATGTCCCAGAAGATGTTGCACAAGAAATGGTAGAGTCTGGGTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGACAGAGTATCATTAATTAAGAGGAAACGAGAGCAGCGGCAGTTGGTACGGGAGGAGCAAGAAAAAAAAAAGCAGGAAGAGAGCAGTCTCAAACAGCAGGTAGAACAATCCAGTGCTTCCCAGACAGGAATCAAGCAGCTCCCTTCTGCTAGCACCGGCATACCTACTGCTTCTACCACTTCAGCTTCAGTTTCTACACAAGTAGAACCTGAAGAACCTGAGGCAGATCAACATCAGCAACTACAGTACCAGCAACCCAGTATATCTGTGTTATCTGATGGGACGGTTGACAGTGGTCAGGGATCCTCTGTCTTCACAGAATCTCGAGTGAGCAGCCAACAGACAGTTTCATATGGTTCCCAACATGAACAGGCACATTCTACAGGCACAGTCCCAGGGCATATACCTTCTACTGTCCAAGCACAGTCTCAGCCCCATGGGGTATATCCACCCTCAAGTGTGCCTCAGTCCATGGCGCATCCGTGTGGGGGGACCCCAACATACCCAGAATCACAGATATTTTTCCCAACTATTCATGAACGTCCAGTTTCTTTTTCACCACCTCCCACCTGCCCACCGAAAGTAGCCATTTCCCAGCGGCGTAAGAGCACCTCCTTCCTGGAAGCCCAAACTCACCACTTCCAACCCCTGCTGAGGACTGTTGGCCAAAGTCTTCTTCCACCTGGTGGCAGCCCAACTAACTGGACACCAGAGGCCGTAGTTATGTTGGGTACTACAGCCAGTAGAGTAACTGGAGAGTCATGTGAGATACAGGTCCATCCTATGTTTGAACCATCTCAAGTTTACAGTGACTATAGACCTGGACTAGTACTTCCAGAAGAAGCTCACTATTTTATTCCTCAGGAAGCAGTGTATGTAGCTGGGGTACATTACCAGGCCCGGGTGGCAGAACAGTATGAGGGCATTCCATACAACTCATCAGTACTGTCAAGTCCTATGAAACAGATACCTGAACAGAAGCCAGTACAAGGGGGCCCTACTTCAAGTTCTGTCTTTGAATTTCCATCTGGACAGGCTTTCCTGGTAGGACACCTTCAGAATCTAAGATTAGATTCTGGATTGGGTCCGGGATCTCCCCTCTCTAGTATTTCTGCACCTATCAGTACAGATGCTACACGTTTGAAATTTCACCCTGTCTTTGTTCCTCATTCTGCGCCTGCTGTGTTAACTCATAACAATGAGAGCAGAAGCAACTGTGTATTTGAATTTCATGTTCACACACCAAGCTCCTCTTCAGGAGAAGGAGGTGGAATTTTACCTCAGCGTGTTTACCGAAATCGGCAGGTTGCAGTGGACTTGAATCAAGAAGAACTGCCTCCTCAATCAGTTGGATTACATGGCTACTTGCAGCCTGTGACTGAAGAAAAGCATAATTACCATGCCCCAGAATTGACCGTTTCTGTGGTAGAGCCTATCGGACAGAACTGGCCAATAGGAAGCCCAGAATATTCCAGTGATTCCTCACAAATCACTTCTTCAGACCCCAGTGATTTTCAGTCACCTCCCCCTACAGGGGGAGCAGCTGCACCTTTTGGCTCTGACGTCTCAATGCCCTTTATCCATCTGCCTCAGACAGTGTTACAAGAATCCCCACTTTTCTTCTGTTTCCCCCAAGGAACCACATCTCAGCAGGTCTTAACTGCCTCATTTTCTTCAGGAGGATCTGCACTTCATCCACAGGCACAGGGGCAGAGCCAGGGTCAGCCATCCTCAAGTAGCTTAACAGGGGTTTCATCTTCCCAACCCATACAACATCCTCAGCAGCAGCAGGGAATACAGCAGACAGCCCCTCCTCAACAGACAGTGCAGTATTCACTTTCACAGACATCAACCTCCAGTGAGGCCACTACTGCACAGCCAGTGAGTCAGCCTCAAGCTCCACAAGTCTTGCCTCAAGTATCAGCTGGAAAACAGGGCTTCCCACCTCGACTGCCACCACAGTACCCAGGAGATTCAAATATTGCTCCCTCTTCCAACGTGGCTTCTGTTTGCATCCATTCTACAGTCCTATCCCCTCCCATGCCGACAGAAGTACTGGCTACACCTGGGTACTTTCCCACAGTGGTGCAGCCTTATGTGGAATCAAATCTTTTAGTTCCTATGGGTGGTGTAGGAGGACAGGTTCAAGTGTCCCAGCCAGGAGGGAGTTTAGCACAAGCCCCCACTACATCCTCCCAGCAAGCAGTTTTGGAGAGTACTCAGGGAGTCTCTCAGGTTGCTCCTGCAGAGCCAGTTGCAGTAGCACAGCCCCAAGCTACCCAGCCGACCACTTTGGCTTCCTCTGTAGACAGTGCACATTCAGATGTTGCTTCAGGTATGAGTGATGGCAATGAGAACGTCCCATCTTCCAGTGGAAGGCATGAAGGAAGAACTACAAAACGGCATTACCGAAAATCTGTAAGGAGTCGCTCTCGACATGAAAAAACTTCACGCCCAAAATTAAGAATTTTGAATGTTTCAAATAAAGGAGACCGAGTAGTAGAATGTCAATTAGAGACTCATAATAGGAAAATGGTTACATTCAAATTTGACCTAGATGGTGACAACCCCGAGGAGATAGCAACAATTATGGTGAACAATGACTTTATTCTAGCAATAGAGAGAGAGTCGTTTGTGGATCAAGTGCGAGAAATTATTGAAAAAGCTGATGAAATGCTCAGTGAGGATGTCAGTGTGGAACCAGAGGGTGATCAGGGATTGGAGAGTCTACAAGGAAAGGATGACTATGGCTTTTCAGGTTCTCAGAAGCTTGAAGGAGAGTTCAAACAACCAATTCCTGCGTCTTCCATGCCACAGCAAATAGGCATTCCTACCAGTTCTTTAACTCAAGTTGTTCATTCTGCGGGAAGGCGGTTTATAGTGAGTCCTGTGCCAGAAAGCCGATTACGAGAATCAAAAGTTTTCCCCAGTGAAATAACAGATACAGTTGCTGCCTCTACAGCTCAGAGCCCTGGAATGAACTTGTCTCACTCTGCATCATCCCTTAGTCTACAACAGGCCTTTTCTGAACTTAGACGTGCCCAAATGACAGAAGGACCCAACACAGCACCTCCAAACTTTAGTCATACAGGACCAACATTTCCAGTAGTACCTCCTTTCTTAAGTAGCATTGCTGGAGTCCCAACCACAGCAGCAGCCACAGCACCAGTCCCTGCAACAAGCAGCCCTCCTAATGACATTTCCACATCAGTAATTCAGTCTGAGGTTACAGTGCCCACTGAAGAGGGGATTGCTGGAGTTGCCACCAGCACAGGTGTGGTAACTTCGGGTGGTCTCCCCATACCACCTGTGTCTGAATCACCAGTACTTTCCAGCGTAGTTTCAAGTATCACAATACCTGCAGTTGTCTCAATATCTACTACATCCCCGTCACTTCAAGTCCCCACATCCACATCTGAGATCGTTGTTTCTAGTACAGCACTGTATCCTTCAGTAACAGTTTCAGCAACTTCAGCCTCTGCAGGGGGCAGTACTGCTACCCCAGGTCCTAAGCCTCCAGCTGTAGTATCTCAGCAGGCAGCAGGCAGCACTACTGTGGGAGCCACATTAACATCAGTTTCTACCACCACTTCATTCCCAAGCACAGCTTCACAGCTGTCCATTCAGCTTAGCAGCAGTACTTCTACTCCTACTTTAGCTGAAACCGTGGTAGTTAGCGCACACTCACTAGATAAGACATCTCATAGCAGTACAACTGGATTGGCTTTCTCCCTCTCTGCACCATCTTCCTCTTCCTCTCCTGGAGCAGGAGTGTCTAGTTATATTTCTCAGCCTGGTGGGCTGCATCCTTTGGTCATTCCATCAGTGATAGCTTCTACTCCTATTCTTCCCCAAGCAGCAGGACCTACTTCTACACCTTTATTACCCCAAGTACCTAGTATCCCACCCTTGGTACAGCCTGTTGCCAATGTGCCTGCTGTACAGCAGACACTAATTCATAGTCAGCCTCAACCAGCTTTGCTTCCCAACCAGCCCCATACTCATTGTCCTGAAGTAGATTCTGATACACAACCCAAAGCTCCTGGAATTGATGACATAAAGACTCTAGAAGAAAAGCTGCGGTCTCTGTTCAGTGAACACAGCTCATCTGGAGCTCAGCATGCCTCTGTCTCACTGGAGACCTCACTAGTCATAGAGAGCACTGTCACACCAGGCATCCCAACTACTGCTGTTGCACCAAGCAAACTCCTGACTTCTACCACAAGTACTTGCTTACCACCAACCAATTTACCACTAGGAACAGTTGCTTTGCCAGTTACACCAGTGGTCACACCTGGGCAAGTTTCTACCCCAGTCAGCACTACTACATCAGGAGTGAAACCTGGAACTGCTCCCTCCAAGCCACCTCTAACTAAGGCTCCGGTGCTGCCAGTGGGTACTGAACTTCCAGCAGGTACTCTACCCAGCGAGCAGCTGCCACCTTTTCCAGGACCTTCTCTAACCCAGTCCCAGCAACCTCTAGAGGATCTTGATGCTCAATTGAGAAGAACACTTAGTCCAGAGATTATCACAGTGACTTCTGCGGTTGGTCCTGTGTCCATGGCGGCTCCAACAGCAATCACAGAAGCAGGAACACAGCCTCAGAAGGGTGTTTCTCAAGTCAAAGAAGGCCCTGTCCTAGCGACTAGTTCAGGAGCTGGTGTTTTTAAGATGGGACGATTTCAGGTTTCTGTTGCAGCAGACGGTGCCCAGAAAGAGGGTAAAAATAAGTCAGAAGATGCAAAGTCTGTTCATTTTGAATCCAGCACCTCAGAGTCCTCAGTGCTATCAAGTAGTAGTCCAGAGAGTACCTTGGTGAAACCAGAGCCGAATGGCATAACCATCCCTGGTATCTCTTCAGATGTGCCAGAGAGTGCCCACAAAACTACTGCCTCAGAGGCAAAGTCAGACACTGGGCAGCCTACCAAGGTTGGACGTTTTCAGGTGACAACTACAGCAAACAAAGTGGGTCGTTTCTCTGTATCAAAAACTGAGGACAAGATCACTGACACAAAGAAAGAAGGACCAGTGGCATCTCCTCCTTTTATGGATTTGGAACAAGCTGTTCTTCCTGCTGTGATACCAAAGAAAGAGAAGCCTGAACTGTCAGAGCCTTCACATCTAAATGGGCCGTCTTCTGACCCGGAGGCCGCTTTTTTAAGTAGGGATGTGGATGATGGTTCCGGTAGTCCACACTCGCCCCATCAGCTGAGCTCAAAGAGCCTTCCTAGCCAGAATCTAAGTCAAAGCCTTAGTAATTCATTTAACTCCTCTTACATGAGTAGCGACAATGAGTCAGATATCGAAGATGAAGACTTAAAGTTAGAGCTGCGACGACTACGAGATAAACATCTCAAAGAGATTCAGGACCTGCAGAGTCGCCAGAAGCATGAAATTGAATCTTTGTATACCAAACTGGGCAAGGTGCCCCCTGCTGTTATTATTCCCCCAGCTGCTCCCCTTTCAGGGAGAAGACGACGACCCACTAAAAGCAAAGGCAGCAAATCTAGTCGAAGCAGTTCCTTGGGGAATAAAAGCCCCCAGCTTTCAGGTAACCTGTCTGGTCAGAGTGCAGCTTCAGTCTTGCACCCCCAGCAGACCCTCCACCCTCCTGGCAACATCCCAGAGTCCGGGCAGAATCAGCTGTTACAGCCCCTTAAGCCATCTCCCTCCAGTGACAACCTCTATTCAGCCTTCACCAGTGATGGTGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCAAGGAACCAGCAGCACAAACACTGTTGGGGCAACAGTGAACAGCCAAGCCGCCCAAGCTCAGCCTCCTGCCATGACGTCCAGCAGGAAGGGCACATTCACAGATGACTTGCACAAGTTGGTAGACAATTGGGCCCGAGATGCCATGAATCTCTCAGGCAGGAGAGGAAGCAAAGGGCACATGAATTATGAGGGCCCTGGAATGGCAAGGAAGTTCTCTGCACCTGGGCAACTGTGCATCTCCATGACCTCGAACCTGGGTGGCTCTGCCCCCATCTCTGCAGCATCAGCTACCTCTCTAGGTCACTTCACCAAGTCTATGTGCCCCCCACAGCAGTATGGCTTTCCAGCTACCCCATTTGGCGCTCAATGGAGTGGGACGGGTGGCCCAGCACCACAGCCACTTGGCCAGTTCCAACCTGTGGGAACTGCCTCCTTGCAGAATTTCAACATCAGCAATTTGCAGAAATCCATCAGCAACCCCCCAGGCTCCAACCTGCGGACCACTTAGGTCGAC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSEFMSGGAAEKQSS
TPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTM
DKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAV
PQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTS
KDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKA
VGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSE
RQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKT
YLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPT
GSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMC
MLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIR
QNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKK
LKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDR
VSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSAST
GIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGS
SVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPS
SVPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRK
STSFLEAQTHHFQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTG
ESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARV
AEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHL
QNLRLDSGLGPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNES
RSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGL
HGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDP
SDFQSPPPTGGAAAPFGSDVSMPFIHLPQTVLQESPLFFCFPQGTTSQQV
LTASFSSGGSALHPQAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQT
APPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQGFPPRLP
PQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVES
NLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPV
AVAQPQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRH
YRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDL
DGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPE
GDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVV
HSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSL
SLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAA
ATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIP
PVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSV
TVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPST
ASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSS
SPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIP
PLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDD
IKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSK
LLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAP
SKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLR
RTLSPEIITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSG
AGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPE
STLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTT
ANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPEL
SEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLS
NSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESL
YTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGN
LSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSD
GAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLH
KLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGG
SAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQF
QPVGTASLQNFNISNLQKSISNPPGSNLRTT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48134
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Variant 4. Contains 3 amino acid changes as compared to the reference sequence these are however known variations and designated as benign (ensembl); T1316P, C1766S and M2068I. Contains various silent nucleotide changes as compared to the reference sequence; T>C 258 bp, A>G 1287 bp, A>G 1914 bp, A>G 4446 bp, T>C 4447 bp, G>T 4449 bp, A>G 4965 bp, A>G 6321 bp and C>T 7608 bp.; Grow at 30oC.