cDNA Clone BRD4

• Plasmid: pRETROX Tight HA BRD4
• Parent Plasmid: pRetroX tight puro
• Expressed: HA BRD4
• Genbank: NM_058243.2
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGTCTGCGGAGAGCGGCCCTGGGACGAGATTGAGAAATCTGCC AGTAATGGGGGATGGACTAGAAACTTCCCAAATGTCTACAACACAGGCCC AGGCCCAACCCCAGCCAGCCAACGCAGCCAGCACCAACCCCCCGCCCCCA GAGACCTCCAACCCTAACAAGCCCAAGAGGCAGACCAACCAACTGCAATA CCTGCTCAGAGTGGTGCTCAAGACACTATGGAAACACCAGTTTGCATGGC CTTTCCAGCAGCCTGTGGATGCCGTCAAGCTGAACCTCCCTGATTACTAT AAGATCATTAAAACGCCTATGGATATGGGAACAATAAAGAAGCGCTTGGA AAACAACTATTACTGGAATGCTCAGGAATGTATCCAGGACTTCAACACTA TGTTTACAAATTGTTACATCTACAACAAGCCTGGAGATGACATAGTCTTA ATGGCAGAAGCTCTGGAAAAGCTCTTCTTGCAAAAAATAAATGAGCTACC CACAGAAGAAACCGAGATCATGATAGTCCAGGCAAAAGGAAGAGGACGTG GGAGGAAAGAAACAGGGACAGCAAAACCTGGCGTTTCCACGGTACCAAAC ACAACTCAAGCATCGACTCCTCCGCAGACCCAGACCCCTCAGCCGAATCC TCCTCCTGTGCAGGCCACGCCTCACCCCTTCCCTGCCGTCACCCCGGACC TCATCGTCCAGACCCCTGTCATGACAGTGGTGCCTCCCCAGCCACTGCAG ACGCCCCCGCCAGTGCCCCCCCAGCCACAACCCCCACCCGCTCCAGCTCC CCAGCCCGTACAGAGCCACCCACCCATCATCGCGGCCACCCCACAGCCTG TGAAGACAAAGAAGGGAGTGAAGAGGAAAGCAGACACCACCACCCCCACC ACCATTGACCCCATTCACGAGCCACCCTCGCTGCCCCCGGAGCCCAAGAC CACCAAGCTGGGCCAGCGGCGGGAGAGCAGCCGGCCTGTGAAACCTCCAA AGAAGGACGTGCCCGACTCTCAGCAGCACCCAGCACCAGAGAAGAGCAGC AAGGTCTCGGAGCAGCTCAAGTGCTGCAGCGGCATCCTCAAGGAGATGTT TGCCAAGAAGCACGCCGCCTACGCCTGGCCCTTCTACAAGCCTGTGGACG TGGAGGCACTGGGCCTACACGACTACTGTGACATCATCAAGCACCCCATG GACATGAGCACAATCAAGTCTAAACTGGAGGCCCGTGAGTACCGTGATGC TCAGGAGTTTGGTGCTGACGTCCGATTGATGTTCTCCAACTGCTATAAGT ACAACCCTCCTGACCATGAGGTGGTGGCCATGGCCCGCAAGCTCCAGGAT GTGTTCGAAATGCGCTTTGCCAAGATGCCGGACGAGCCTGAGGAGCCAGT GGTGGCCGTGTCCTCCCCGGCAGTGCCCCCTCCCACCAAGGTTGTGGCCC CGCCCTCATCCAGCGACAGCAGCAGCGATAGCTCCTCGGACAGTGACAGT TCGACTGATGACTCTGAGGAGGAGCGAGCCCAGCGGCTGGCTGAGCTCCA GGAGCAGCTCAAAGCCGTGCACGAGCAGCTTGCAGCCCTCTCTCAGCCCC AGCAGAACAAACCAAAGAAAAAGGAGAAAGACAAGAAGGAAAAGAAAAAA GAAAAGCACAAAAGGAAAGAGGAAGTGGAAGAGAATAAAAAAAGCAAAGC CAAGGAACCTCCTCCTAAAAAGACGAAGAAAAATAATAGCAGCAACAGCA ATGTGAGCAAGAAGGAGCCAGCGCCCATGAAGAGCAAGCCCCCTCCCACG TATGAGTCGGAGGAAGAGGACAAGTGCAAGCCTATGTCCTATGAGGAGAA GCGGCAGCTCAGCTTGGACATCAACAAGCTCCCCGGCGAGAAGCTGGGCC GCGTGGTGCACATCATCCAGTCACGGGAGCCCTCCCTGAAGAATTCCAAC CCCGACGAGATTGAAATCGACTTTGAGACCCTGAAGCCGTCCACACTGCG TGAGCTGGAGCGCTATGTCACCTCCTGTTTGCGGAAGAAAAGGAAACCTC AAGCTGAGAAAGTTGATGTGATTGCCGGCTCCTCCAAGATGAAGGGCTTC TCGTCCTCAGAGTCGGAGAGCTCCAGTGAGTCCAGCTCCTCTGACAGCGA AGACTCCGAAACAGAGATGGCTCCGAAGTCAAAAAAGAAGGGGCACCCCG GGAGGGAGCAGAAGAAGCACCATCATCACCACCATCAGCAGATGCAGCAG GCCCCGGCTCCTGTGCCCCAGCAGCCGCCCCCGCCTCCCCAGCAGCCCCC ACCGCCTCCACCTCCGCAGCAGCAACAGCAGCCGCCACCCCCGCCTCCCC CACCCTCCATGCCGCAGCAGGCAGCCCCGGCGATGAAGTCCTCGCCCCCA CCCTTCATTGCCACCCAGGTGCCCGTCCTGGAGCCCCAGCTCCCAGGCAG CGTCTTTGACCCCATCGGCCACTTCACCCAGCCCATCCTGCACCTGCCGC AGCCTGAGCTGCCCCCTCACCTGCCCCAGCCGCCTGAGCACAGCACTCCA CCCCATCTCAACCAGCACGCAGTGGTCTCTCCTCCAGCTTTGCACAACGC ACTACCCCAGCAGCCATCACGGCCCAGCAACCGAGCCGCTGCCCTGCCTC CCAAGCCCGCCCGGCCCCCAGCCGTGTCACCAGCCTTGACCCAAACACCC CTGCTCCCACAGCCCCCCATGGCCCAACCCCCCCAAGTGCTGCTGGAGGA TGAAGAGCCACCTGCCCCACCCCTCACCTCCATGCAGATGCAGCTGTACC TGCAGCAGCTGCAGAAGGTGCAGCCCCCTACGCCGCTACTCCCTTCCGTG AAGGTGCAGTCCCAGCCCCCACCCCCCCTGCCGCCCCCACCCCACCCCTC TGTGCAGCAGCAGCTGCAGCAGCAGCCGCCACCACCCCCACCACCCCAGC CCCAGCCTCCACCCCAGCAGCAGCATCAGCCCCCTCCACGGCCCGTGCAC TTGCAGCCCATGCAGTTTTCCACCCACATCCAACAGCCCCCGCCACCCCA GGGCCAGCAGCCCCCCCATCCGCCCCCAGGCCAGCAGCCACCCCCGCCGC AGCCTGCCAAGCCTCAGCAAGTCATCCAGCACCACCATTCACCCCGGCAC CACAAGTCGGACCCCTACTCAACCGGTCACCTCCGCGAAGCCCCCTCCCC GCTTATGATACATTCCCCCCAGATGTCACAGTTCCAGAGCCTGACCCACC AGTCTCCACCCCAGCAAAACGTCCAGCCTAAGAAACAGGAGCTGCGTGCT GCCTCCGTGGTCCAGCCCCAGCCCCTCGTGGTGGTGAAGGAGGAGAAGAT CCACTCACCCATCATCCGCAGCGAGCCCTTCAGCCCCTCGCTGCGGCCGG AGCCCCCCAAGCACCCGGAGAGCATCAAGGCCCCCGTCCACCTGCCCCAG CGGCCGGAAATGAAGCCTGTGGATGTCGGGAGGCCTGTGATCCGGCCCCC AGAGCAGAACGCACCGCCACCAGGGGCCCCTGACAAGGACAAACAGAAAC AGGAGCCGAAGACTCCAGTTGCGCCCAAAAAGGACCTGAAAATCAAGAAC ATGGGCTCCTGGGCCAGCCTAGTGCAGAAGCATCCGACCACCCCCTCCTC CACAGCCAAGTCATCCAGCGACAGCTTCGAGCAGTTCCGCCGCGCCGCTC GGGAGAAAGAGGAGCGTGAGAAGGCCCTGAAGGCTCAGGCCGAGCACGCT GAGAAGGAGAAGGAGCGGCTGCGGCAGGAGCGCATGAGGAGCCGAGAGGA CGAGGATGCGCTGGAGCAGGCCCGGCGGGCCCATGAGGAGGCACGTCGGC GCCAGGAGCAGCAGCAGCAGCAGCGCCAGGAGCAACAGCAGCAGCAGCAA CAGCAAGCAGCTGCGGTGGCTGCCGCCGCCACCCCACAGGCCCAGAGCTC CCAGCCCCAGTCCATGCTGGACCAGCAGAGGGAGTTGGCCCGGAAGCGGG AGCAGGAGCGAAGACGCCGGGAAGCCATGGCAGCTACCATTGACATGAAT TTCCAGAGTGATCTATTGTCAATATTTGAAGAAAATCTTTTCTGAgcggc cgc

Amino Acid Sequence

MYPYDVPDYAGSMSAESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPA
NAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVD
AVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYI
YNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGT
AKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPHPFPAVTPDLIVQTPV
MTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQPVKTKKGV
KRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKPPKKDVPDS
QQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLH
DYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHE
VVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDS
SSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKK
KEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEP
APMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQ
SREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDV
IAGSSKMKGFSSSESESSSESSSSDSEDSETEMAPKSKKKGHPGREQKKH
HHHHHQQMQQAPAPVPQQPPPPPQQPPPPPPPQQQQQPPPPPPPPSMPQQ
AAPAMKSSPPPFIATQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPH
LPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPARPP
AVSPALTQTPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKV
QPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQLQQQPPPPPPPQPQPPPQQ
QHQPPPRPVHLQPMQFSTHIQQPPPPQGQQPPHPPPGQQPPPPQPAKPQQ
VIQHHHSPRHHKSDPYSTGHLREAPSPLMIHSPQMSQFQSLTHQSPPQQN
VQPKKQELRAASVVQPQPLVVVKEEKIHSPIIRSEPFSPSLRPEPPKHPE
SIKAPVHLPQRPEMKPVDVGRPVIRPPEQNAPPPGAPDKDKQKQEPKTPV
APKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEQFRRAAREKEERE
KALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQ
QRQEQQQQQQQQAAAVAAAATPQAQSSQPQSMLDQQRELARKREQERRRR
EAMAATIDMNFQSDLLSIFEENLF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU46347
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: in pRETROX tight