cDNA Clone ARNT

• Synonyms: Hif1beta
• Plasmid: pcDNA5D FRT/TO -mCherry-ARNT
• Parent Plasmid: pcDNA5D FRT/TO mCherry
• Expressed: mCherry-ARNT
• Genbank: Gene 405
• Tag: mCherry
• Species: Human

Sequence of Insert

ggatccATGGCGGCGACTACTGCCAACCCCGAAATGACATCAGATGTACCATCACT
GGGTCCAGCCATTGCCTCTGGAAACTCTGGACCTGGAATTCAAGGTGGAG
GAGCCATTGTCCAGAGGGCTATTAAGCGGCGACCAGGGCTGGATTTTGAT
GATGATGGAGAAGGGAACAGTAAATTTTTGAGGTGTGATGATGATCAGAT
GTCTAACGATAAGGAGCGGTTTGCCAGGTCGGATGATGAGCAGAGCTCTG
CGGATAAAGAGAGACTTGCCAGGGAAAATCACAGTGAAATTGAACGGCGG
CGACGGAACAAGATGACAGCCTACATCACAGAACTGTCAGATATGGTACC
CACCTGTAGTGCCCTGGCTCGAAAACCAGACAAGCTAACCATCTTACGCA
TGGCAGTTTCTCACATGAAGTCCTTGCGGGGAACTGGCAACACATCCACT
GATGGCTCCTATAAGCCGTCTTTCCTCACTGATCAGGAACTGAAACATTT
GATCTTGGAGGCAGCAGATGGCTTTCTGTTTATTGTCTCATGTGAGACAG
GCAGGGTGGTGTATGTCTCTGACTCCGTGACTCCTGTTTTGAACCAGCCA
CAGTCTGAATGGTTTGGCAGCACACTCTATGATCAGGTGCACCCAGATGA
TGTGGATAAACTTCGTGAGCAGCTTTCCACTTCAGAAAATGCCCTGACAG
GGCGTATCCTGGATCTAAAGACTGGAACAGTGAAAAAGGAAGGTCAGCAG
TCTTCCATGAGAATGTGTATGGGCTCAAGGAGATCGTTTATTTGCCGAAT
GAGGTGTGGCAGTAGCTCTGTGGACCCAGTTTCTGTGAATAGGCTGAGCT
TTGTGAGGAACAGATGCAGGAATGGACTTGGCTCTGTAAAGGATGGGGAA
CCTCACTTCGTGGTGGTCCACTGCACAGGCTACATCAAGGCCTGGCCCCC
AGCAGGTGTTTCCCTCCCAGATGATGACCCAGAGGCTGGCCAGGGAAGCA
AGTTTTGCCTAGTGGCCATTGGCAGATTGCAGGTAACTAGTTCTCCCAAC
TGTACAGACATGAGTAATGTTTGTCAACCAACAGAGTTCATCTCCCGACA
CAACATTGAGGGTATCTTCACTTTTGTGGATCACCGCTGTGTGGCTACTG
TTGGCTACCAGCCACAGGAACTCTTAGGAAAGAATATTGTAGAATTCTGT
CATCCTGAAGACCAGCAGCTTCTAAGAGACAGCTTCCAACAGGTAGTGAA
ATTAAAAGGCCAAGTGCTGTCTGTCATGTTCCGGTTCCGGTCTAAGAACC
AAGAATGGCTCTGGATGAGAACCAGCTCCTTTACTTTCCAGAACCCTTAC
TCAGATGAAATTGAGTACATCATCTGTACCAACACCAATGTGAAGAACTC
TAGCCAAGAACCACGGCCTACACTCTCCAACACAATCCAGAGGCCACAAC
TAGGTCCCACAGCTAATTTACCCCTGGAGATGGGCTCAGGACAGCTGGCA
CCCAGGCAGCAGCAACAGCAAACAGAATTGGACATGGTACCAGGAAGAGA
TGGACTGGCCAGCTACAATCATTCCCAGGTGGTTCAGCCTGTGACAACCA
CAGGACCAGAACACAGCAAGCCCCTTGAGAAGTCAGATGGTTTATTTGCC
CAGGATAGAGATCCAAGATTTTCAGAAATCTATCACAACATCAATGCGGA
TCAGAGTAAAGGCATCTCCTCCAGCACTGTCCCTGCCACCCAACAGCTAT
TCTCCCAGGGCAACACATTCCCTCCTACCCCCCGGCCGGCAGAGAATTTC
AGGAATAGTGGCCTAGCCCCTCCTGTAACCATTGTCCAGCCATCAGCTTC
TGCAGGACAGATGTTGGCCCAGATTTCCCGCCACTCCAACCCCACCCAAG
GAGCAACCCCAACTTGGACCCCTACTACCCGCTCAGGCTTTTCTGCCCAG
CAGGTGGCTACCCAGGCTACTGCTAAGACTCGTACTTCCCAGTTTGGTGT
GGGCAGCTTTCAGACTCCATCCTCCTTCAGCTCCATGTCCCTCCCTGGTG
CCCCAACTGCATCGCCTGGTGCTGCTGCCTACCCTAGTCTCACCAATCGT
GGATCTAACTTTGCTCCTGAGACTGGACAGACTGCAGGACAATTCCAGAC
ACGGACAGCAGAGGGTGTGGGTGTCTGGCCACAGTGGCAGGGCCAGCAGC
CTCATCATCGTTCAAGTTCTAGTGAGCAACATGTTCAACAACCGCCAGCA
CAGCAACCTGGCCAGCCTGAGGTCTTCCAGGAGATGCTGTCCATGCTGGG
AGATCAGAGCAACAGCTACAACAATGAAGAATTCCCTGATCTAACTATGT
TTCCCCCCTTTTCAGAATAGgcggccgc

Amino Acid Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWER
VMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEA
SSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV
NIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKSGLGSMAATTANPE
MTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKF
LRCDDDQMSNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYI
TELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFL
TDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQPQSEWFGSTL
YDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGS
RRSFICRMRCGSSSVDPVSVNRLSFVRNRCRNGLGSVKDGEPHFVVVHCT
GYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSSPNCTDMSNVCQ
PTEFISRHNIEGIFTFVDHRCVATVGYQPQELLGKNIVEFCHPEDQQLLR
DSFQQVVKLKGQVLSVMFRFRSKNQEWLWMRTSSFTFQNPYSDEIEYIIC
TNTNVKNSSQEPRPTLSNTIQRPQLGPTANLPLEMGSGQLAPRQQQQQTE
LDMVPGRDGLASYNHSQVVQPVTTTGPEHSKPLEKSDGLFAQDRDPRFSE
IYHNINADQSKGISSSTVPATQQLFSQGNTFPPTPRPAENFRNSGLAPPV
TIVQPSASAGQMLAQISRHSNPTQGATPTWTPTTRSGFSAQQVATQATAK
TRTSQFGVGSFQTPSSFSSMSLPGAPTASPGAAAYPSLTNRGSNFAPETG
QTAGQFQTRTAEGVGVWPQWQGQQPHHRSSSSEQHVQQPPAQQPGQPEVF
QEMLSMLGDQSNSYNNEEFPDLTMFPPFSE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24381
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Clone harbours silent mutation at V189 (GTG to GTC) compared to NCBI ref. Parental 41458