cDNA Clone UBE2S

• Synonyms: E2-EPF, E2EPF, EPF5
• Plasmid: pMA UBE2S Nter Puro
• Parent Plasmid: pMA
• Genbank: ENSG00000108106
• Species: Human

Sequence of Insert

TCAAACGGTCCAATCAGCGGCCGCGTCGCCTTCCCCGTCCCCCTTCCTCTCTTCTTCCCCTTTTCAGCCCCTCCGTTCTTTGAGAACCCTCCCGACCCCCGCGGGCCCCCATTGGCCGGCCCGCTACCCAGCCCTCTCCGCCACTTCCCTCGCTTCTGACCATAGTTTGCGGGGAAGGGAGCGAGCGCGTCGAAAACCAAGGAACGTGCGCGCTGACGTCACGGTTGAGGCTCGGAGCTGAGGGGCCGCGGAGGGCGTGGCCTGCGGGCGGTTATAAAGAGGCAGTGGTGCGCGCGCGGCCGGCTCAGTGCTGCCGGGCACCGGGGCGGCGGGTTGGTCTACGCTGTGCGCGGCGGACGTCGGAGGCAGCGGGGAGCGGAGCGGGGCCGCCGGGGCCTCTCCAGGGCCGCAGCGGCAGCAGTTGGGCCCCCCGCCCCGGCCGGCGGACCGAAGAACGCAGGAAGGGGGCCGGGGGGACCCGCCCCCGGCCGGCCGCAGCCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGTGCCCGAAGGACCGCGCGACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAGTGAGCACGGGCGATGAGGCCCATGAAGGTGGTCGGGGGTGTCCCTGAGGGAGGAGGTGGAGTCTCAGGGGCCTCCGACGGGCGGCCTTGAGGGGTTTGAGGGCCAGCCTGGGGGTCCCTGACCCGGGATGGGCCCGAGGGCCTTCCCGCAGGCGGAGCAGGAGCTCGGGCGGCCCGCAGTCTCCCTGGCGGCGAGGGCTCCGGGCGCTTTGCGGCCCGCCCATGGGTGTTACCAGGAGCAAGGACGCCCCGCCGCCATCTCCAGACCTGAGCCGGGGTTTCAAGGATGCTCTAACCAAAAATTAGTGGAGAAGGGGGAGAGAGTAGATCTCTGGCCTACGTGGAACCACTGGGGACCAAGCAGGGTTAGGAGCGGGCTTTGGGGGGGGGGTTCTGGGGTGGGTGGAGTTATCTGCAAGCCCCCCCGTGAACCCGCCCCCATTTCTGTTGGAAACTCTGCCTGGGCTGATGGCCTGAAGCATCCTGGGTGAGGGTCTGGGcggccgc

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48148
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Designed to replace the UBE2S coding region of exon 1 with puromycin. Complete sequence of donor including homology arms shown as sequence of insert. Requires modification to add the BGH polyA signl to prevent NMD.