cDNA Clone SV2A

• Synonyms: none
• Plasmid: pET156P SV2A M1-G160
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-SV2A M1-G160
• Genbank: NM_014849.4
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccATGGAAGAGGGCTTCCGAGACCGGGCAGCTTTCATCCGTGGGGCCAAAGACATTGCTAAGGAAGTCAAAAAGCATGCGGCCAAGAAGGTGGTGAAGGGCCTGGACAGAGTCCAGGACGAATATTCCCGAAGATCGTACTCCCGCTTTGAGGAGGAGGATGATGATGATGACTTCCCTGCTCCCAGTGATGGTTATTACCGAGGAGAAGGGACCCAGGATGAGGAGGAAGGTGGTGCATCCAGTGATGCTACTGAGGGCCATGACGAGGATGATGAGATCTATGAAGGGGAATATCAGGGCATTCCCCGGGCAGAGTCTGGGGGCAAAGGCGAGCGGATGGCAGATGGGGCGCCCCTGGCTGGAGTAAGGGGGGGCTTGAGTGATGGGGAGGGTCCCCCTGGGGGCCGGGGGGAGGCACAACGACGGAAAGAACGAGAAGAACTGGCCCAACAGTATGAAGCCATCCTACGGGAGTGTGGCTAGGGCCGCTTCCAGTGGACACTGTATTTTGTGCTTGGTCTGGCGCTGATGGCTGACGGTGTGGAGGTCTTTGTGGTGGGCTTCGTGCTGCCCAGCGCTGAGAAAGACATGTGCCTGTCCGACTCCAACAAAGGCATGCTAGGCCTCATCGTCTACCTGGGCATGATGGTGGGAGCCTTCCTCTGGGGAGGTCTGGCTGACCGGCTGGGTCGGAGGCAGTGTCTGCTCATCTCGCTCTCAGTCAACAGCGTCTTCGCCTTCTTCTCATCTTTTGTCCAGGGTTACGGCACTTTCCTCTTCTGCCGCCTACTTTCTGGGGTTGGGATTGGAGGGTCCATCCCCATTGTCTTCTCCTATTTCTCCGAGTTTCTGGCCCAGGAGAAACGAGGGGAGCATTTGAGCTGGCTCTGCATGTTTTGGATGATTGGTGGCGTGTACGCAGCTGCTATGGCCTGGGCCATCATCCCCCACTATGGGTGGAGTTTTCAGATGGGTTCTGCCTACCAGTTCCACAGCTGGAGGGTCTTCGTCCTCGTCTGCGCCTTTCCTTCTGTGTTTGCCATTGGGGCTCTGACCACGCAGCCTGAGAGCCCCCGTTTCTTCCTAGAGAATGGAAAGCATGATGAGGCCTGGATGGTGCTGAAGCAGGTCCATGATACCAACATGCGAGCCAAAGGACATCCTGAGCGAGTGTTCTCAGTAACCCACATTAAGACGATTCATCAGGAGGATGAATTGATTGAGATCCAGTCGGACACAGGGACCTGGTACCAGCGCTGGGGGGTCCGGGCCTTGAGCCTAGGGGGGCAGGTTTGGGGGAATTTTCTCTCCTGTTTTGGTCCCGAATATCGGCGCATCACTCTGATGATGATGGGTGTGTGGTTCACCATGTCATTCAGCTACTATGGCCTGACCGTCTGGTTTCCTGACATGATCCGCCATCTCCAGGCAGTGGACTACGCATCCCGCACCAAAGTGTTCCCCGGGGAGCGCGTAGAGCATGTAACTTTTAACTTCACGTTGGAGAATCAGATCCACCGAGGCGGGCAGTACTTCAATGACAAGTTCATTGGGCTGCGGCTCAAGTCAGTGTCCTTTGAGGATTCCCTGTTTGAAGAGTGTTATTTTGAGGATGTCACATCCAGCAACACGTTTTTCCGCAACTGCACATTCATCAACACTGTGTTCTATAACACTGACCTGTTCGAGTACAAGTTTGTGAACAGCCGTCTGATAAACAGTACATTCCTGCACAACAAGGAGGGCTGCCCGCTAGACGTGACAGGGACGGGCGAAGGTGCCTACATGGTATACTTTGTGAGCTTCCTGGGGACACTGGCAGTGCTTCCTGGGAATATCGTGTCTGCCCTGCTCATGGACAAGATCGGCAGGCTCAGAATGCTTGCTGGCTCCAGCGTGATGTCCTGTGTCTCCTGCTTCTTCCTGTCTTTTGGGAACAGTGAGTCGGCCATGATCGCTCTGCTCTGCCTTTTTGGCGGGGTCAGCATTGCATCCTGGAATGCGCTGGACGTGTTGACTGTTGAACTCTACCCCTCAGACAAGAGGACCACAGCTTTTGGCTTCCTGAATGCCCTGTGTAAGCTGGCAGCTGTGCTGGGGATCAGCATCTTCACATCCTTCGTGGGAATCACCAAGGCTGCACCCATCCTCTTTGCCTCAGCTGCCCTTGCCCTTGGCAGCTCTCTGGCCCTGAAGCTGCCTGAGACCCGGGGGCAGGTGCTGCAGTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMEEGFRDRAAFIRGAKDIAKEVKKHAAKKVVKGLDRVQDEYSRRSYSRFEEEDDDDDFPAPSDGYYRGEGTQDEEEGGASSDATEGHDEDDEIYEGEYQGIPRAESGGKGERMADGAPLAGVRGGLSDGEGPPGGRGEAQRRKEREELAQQYEAILRECG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU25445
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Insert sequence is full length sequence with stop at aa161 therefore creating fragment aa1-160. ; Parental 49269
• Low Temp: No