cDNA Clone MAP4K2

• Synonyms: GCK
• Plasmid: pFBHTc GCK
• Parent Plasmid: pFastbac HTc
• Genbank: BC047865
• Tag: 6His
• Species: Human

Sequence of Insert

GTCGACGCGCTGCTGCGGGATGTGTCGCTGCAGGACCCGCGGGACCGCTT
CGAGCTGCTGCAGCGCGTGGGGGCCGGGACCTATGGCGACGTCTACAAGG
CCCGCGACACGGTCACGTCCGAACTGGCCGCCGTGAAGATAGTCAAGCTA
GGCCCAGGGGACGACATCAGCTCCCTCCAGCAGGAAATCACCATCCTGCG
TGAGTGCCGCCACCCCAATGTGGTGGCCTACATTGGCAGCTACCTCAGGA
ATGACCGCTTGTGGATCTGCATGGAGTTCTGCGGAGGGGGCTCCCTGCAG
GAGATTTACCATGCCACTGGGCCCCTGGAGGAGCGGCAGATTGCCTACGT
CTGCCGAGAGGCACTGAAGGGGCTCCACCACCTGCATTCTCAGGGGAAGA
TCCACAGAGACATCAAGGGAGCCAACCTTCTCCTCACTCTCCAGGGAGAT
GTCAAACTGGCTGACTTTGGGGTGTCAGGCGAGCTGACAGCGTCTGTGGC
CAAGAGGAGGTCTTTCATTGGGACTCCCTACTGGATGGCTCCCGAGGTGG
CTGCTGTGGAGCGCAAAGGTGGCTACAATGAGCTATGTGACGTCTGGGCC
CTGGGCATCACTGCCATTGAGCTGGGCGAGCTGCAGCCCCCTCTGTTCCA
CCTGCACCCCATGAGGGCCCTGATGCTCATGTCGAAGAGCAGCTTCCAGC
CGCCCAAACTGAGAGATAAGACTCGCTGGACCCAGAATTTCCACCACTTT
CTCAAACTGGCCCTGACCAAGAATCCTAAGAAGAGGCCGACAGCAGAGAA
GCTCCTGCAGCACCCGTTCACGACTCAGCAGCTCCCTCGGGCCCTCCTCA
CACAGCTGCTGGACAAAGCCAGTGACCCTCATCTGGGGACCCCCTCCCCT
GAGGACTGTGAGCTGGAGACCTATGACATGTTTCCAGACACCATTCACTC
CCGGGGGCAGCACGGCCCAGCCGAGAGGACCCCCTCGGAGATCCAGTTTC
ACCAGGTGAAATTTGGCGCCCCACGCAGGAAGGAAACTGACCCACTGAAT
GAGCCGTGGGAGGAAGAGTGGACACTACTGGGAAAGGAAGAGTTGAGTGG
GAGCCTGCTGCAGTCGGTCCAGGAGGCCCTGGAGGAAAGGAGTCTGACTA
TTCGGTCAGCCTCAGAATTCCAGGAGCTGGACTCCCCAGACGATACCATG
GGAACCATCAAGCGGGCCCCGTTCCTAGGGCCACTCCCCACTGACCCTCC
AGCAGAGGAGCCTCTGTCCAGTCCCCCAGGCCCCAACAGCTCCCCACTGC
TGCCCACGGCCTGGGCCACCATGAAGCAGCGGGAGGATCCTGAGAGGTCA
TCCTGCCACGGGCTCCCCCCAACTCCCAAGGTGCATATGGGCGCCTGCTT
CTCCAAGGTCTTCAATGGCTGCCCCCTGCGGATCCACGCTGCTGTCACCT
GGATTCACCCTGTTACTCGGGACCAGTTCCTGGTGGTAGGGGCCGAGGAA
GGCATCTACACACTCAACCTGCATGAACTGCATGAGGATACGCTGGAGAA
GCTGATTTCACATCGCTGCTCCTGGCTCTACTGCGTGAACAACGTGCTGC
TGTCACTCTCAGGGAAATCCACGCACATCTGGGCCCATGACCTCCCAGGC
CTGTTTGAGCAGCGGAGGCTACAGCAACAGGTTCCCCTCTCCATCCCCAC
CAACCGCCTCACCCAGCGCATCATCCCCAGGCGCTTTGCTCTGTCCACCA
AGATTCCTGACACCAAAGGCTGCTTGCAGTGTCGTGTGGTGCGGAACCCC
TACACGGGTGCCACCTTCCTGCTGGCCGCCCTGCCCACCAGCCTGCTCCT
GCTGCAGTGGTATGAGCCGCTGCAGAAGTTTCTGCTGCTGAAGAACTTCT
CCAGCCCTCTGCCCAGCCCAGCTGGGATGCTGGAGCCGCTGGTGCTGGAT
GGGAAGGAGCTGCCGCAGGTGTGTGTTGGGGCCGAGGGGCCTGAGGGGCC
CGGCTGCCGCGTCCTGTTCCATGTCCTGCCCCTGGAGGCTGGCCTGACGC
CCGACATCCTCATCCCACCTGAGGGGATCCCAGGCTCGGCCCAGCAGGTG
ATCCAGGTGGACAGGGACACAATCCTAGTCAGCTTTGAACGCTGTGTGAG
GATTGTCAACATGCAGGGCGAGCCCACGGCCACACTGGCACCTGAGCTGA
CCTTTGATTTCCCCATCGAGACTGTGGTGTGCCTGCAGGACAGTGTGCTG
GCCTTCTGGAGCCATGGGATGCAAGGCCGAAGCCTGGATACCAATGAGGT
GACCCAGGAGATCACAGATGAAACAAGGATCTTCCGAGTGCTTGGGGCCC
ACAGAGACATCATCCTGGAGAGCATTCCCACTGACAACCCAGAGGCGCAC
AGCAACCTCTACATCCTCACGGGCCACCAGAGCACCTACTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDALLRDVSLQDPRDR
FELLQRVGAGTYGDVYKARDTVTSELAAVKIVKLDPGDDISSLQQEITIL
RECRHPNVVAYIGSYLRNDRLWICMEFCGGGSLQEIYHATGPLEERQIAY
VCREALKGLHHLHSQGKIHRDIKGANLLLTLQGDVKLADFGVSGELTASV
AKRRSFIGTPYWMAPEVAAVERKGGYNELCDVWALGITAIELGELQPPLF
HLHPMRALMLMSKSSFQPPKLRDKTRWTQNFHHFLKLALTKNPKKRPTAE
KLLQHPFTTQQLPRALLTQLLDKASDPHLGTPSPEDCELETYDMFPDTIH
SRGQHGPAERTPSEIQFHQVKFGAPRRKETDPLNEPWEEEWTLLGKEELS
GSLLQSVQEALEERSLTIRSASEFQELDSPDDTMGTIKRAPFLGPLPTDP
PAEEPLSSPPGPNSSPLLPTAWATMKQREDPERSSCHGLPPTPKVHMGAC
FSKVFNGCPLRIHAAVTWIHPVTRDQFLVVGAEEGIYTLNLHELHEDTLE
KLISHRCSWLYCVNNVLLSLSGKSTHIWAHDLPGLFEQRRLQQQVPLSIP
TNRLTQRIIPRRFALSTKIPDTKGCLQCRVVRNPYTGATFLLAALPTSLL
LLQWYEPLQKFLLLKNFSSPLPSPAGMLEPLVLDGKELPQVCVGAEGPEG
PGCRVLFHVLPLEAGLTPDILIPPEGIPGSAQQVIQVDRDTILVSFERCV
RIVNMQGEPTATLAPELTFDFPIETVVCLQDSVLAFWSHGMQGRSLDTNE
VTQEITDETRIFRVLGAHRDIILESIPTDNPEAHSNLYILTGHQSTY*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1760
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His tag for baculovirus expression.
• Low Temp: No