cDNA Clone TRAF6

• Plasmid: pCMV5 Halo 3C1 Traf6
• Parent Plasmid: pCMV5 Halo 3C1
• Expressed: Halo-TRAF6
• Genbank: NM_004620
• Species: Human

Sequence of Insert

GGATCCAGTCTGCTAAACTGTGAAAACAGCTGTGGATTCAGCCAGTCTGA AAGTGACTGCTGTGTGGCCATGGCCAGCTCCTGTAGCGCTGTAACAAAAG ATGATAGTGTGGGTGGAACTGCCAGCACGGGGAACCTCTCCAGCTCATTT ATGGAGGAGATCCAGGGATATGATGTAGAGTTTGACCCACCCCTGGAAAG CAAGTATGAATGCCCCATCTGCTTGATGGCATTACGAGAAGCAGTGCAAA CGCCATGCGGCCATAGGTTCTGCAAAGCCTGCATCATAAAATCAATAAGG GATGCAGGTCACAAATGTCCAGTTGACAATGAAATACTGCTGGAAAATCA ACTATTTCCAGACAATTTTGCAAAACGTGAGATTCTTTCTCTGATGGTGA AATGTCCAAATGAAGGTTGTTTGCACAAGATGGAACTGAGACATCTTGAG GATCATCAAGCACATTGTGAGTTTGCTCTTATGGATTGTCCCCAATGCCA GCGTCCCTTCCAAAAATTCCATATTAATATTCACATTCTGAAGGATTGTC CAAGGAGACAGGTTTCTTGTGACAACTGTGCTGCATCAATGGCATTTGAA GATAAAGAGATCCATGACCAGAACTGTCCTTTGGCAAATGTCATCTGTGA ATACTGCAATACTATACTCATCAGAGAACAGATGCCTAATCATTATGATC TAGACTGCCCTACAGCCCCAATTCCATGCACATTCAGTACTTTTGGTTGC CATGAAAAGATGCAGAGGAATCACTTGGCACGCCACCTACAAGAGAACAC CCAGTCACACATGAGAATGTTGGCCCAGGCTGTTCATAGTTTGAGCGTTA TACCCGACTCTGGGTATATCTCAGAGGTCCGGAATTTCCAGGAAACTATT CACCAGTTAGAGGGTCGCCTTGTAAGACAAGACCATCAAATCCGGGAGCT GACTGCTAAAATGGAAACTCAGAGTATGTATGTAAGTGAGCTCAAACGAA CCATTCGAACCCTTGAGGACAAAGTTGCTGAAATCGAAGCACAGCAGTGC AATGGAATTTATATTTGGAAGATTGGCAACTTTGGAATGCATTTGAAATG TCAAGAAGAGGAGAAACCTGTTGTGATTCATAGCCCTGGATTCTACACTG GCAAACCCGGGTACAAACTGTGCATGCGCTTGCACCTTCAGTTACCGACT GCTCAGCGCTGTGCAAACTATATATCCCTTTTTGTCCACACAATGCAAGG AGAATATGACAGCCACCTCCCTTGGCCCTTCCAGGGTACAATACGCCTTA CAATTCTTGATCAGTCTGAAGCACCTGTAAGGCAAAACCACGAAGAGATA ATGGATGCCAAACCAGAGCTGCTTGCTTTCCAGCGACCCACAATCCCACG GAACCCAAAAGGTTTTGGCTATGTAACTTTTATGCATCTGGAAGCCCTAA GACAAAGAACTTTCATTAAGGATGACACATTATTAGTGCGCTGTGAGGTC TCCACCCGCTTTGACATGGGTAGCCTTCGGAGGGAGGGTTTTCAGCCACG AAGTACTGATGCAGGGGTATAGGCGGCCGCGC

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRN
IIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQ
AFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPA
EAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGLEV
LFQGPGSMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRN
KKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEIS
MLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYL
NGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYL
KSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSGSSLLNCENSCG
FSQSESDCCVAMASSCSAVTKDDSVGGTASTGNLSSSFMEEIQGYDVEFD
PPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEI
LLENQLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMD
CPQCQRPFQKFHINIHILKDCPRRQVSCDNCAASMAFEDKEIHDQNCPLA
NVICEYCNTILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARH
LQENTQSHMRMLAQAVHSLSVIPDSGYISEVRNFQETIHQLEGRLVRQDH
QIRELTAKMETQSMYVSELKRTIRTLEDKVAEIEAQQCNGIYIWKIGNFG
MHLKCQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFV
HTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPELLAFQR
PTIPRNPKGFGYVTFMHLEALRQRTFIKDDTLLVRCEVSTRFDMGSLRRE
GFQPRSTDAGV*

• Vector Type: Mammalian
• DU Number: DU46423
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Can TRAF6 (DU15444) be cloned into DU39747 to add a Halo tag to the TRAF6. I don't mind if the HA tag is kept on TRAF6 or removed. Thanks.