cDNA Clone Bag6

• Plasmid: pET15b 6HIS SUMO BAG6
• Parent Plasmid: pET15b 6His SUMO
• Expressed: His6-SUMO-Bag6
• Genbank: NM_004639.3
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

ATGGAGCCTAATGATAGTACCAGTACCGCTGTGGAGGAGCCTGACAGCTTGGAGGTGTTGGTGAAGACCTTGGACTCTCAAACTCGTACCTTTATTGTGGGGGCCCAGATGAATGTAAAAGAGTTTAAGGAGCACATTGCTGCCTCTGTCAGCATCCCATCTGAAAAACAACGGCTCATTTACCAGGGACGAGTTCTGCAAGATGATAAGAAGCTTCAGGAATACAATGTTGGGGGAAAGGTTATCCACCTGGTGGAACGGGCTCCTCCTCAGACTCACCTCCCTTCTGGGGCATCTTCTGGGACGGGGTCTGCCTCAGCCACTCATGGTGGGGGATCCCCCCCTGGTACTCGGGGGCCTGGGGCCTCTGTTCATGACCGGAATGCCAACAGCTATGTCATGGTTGGAACCTTCAATCTTCCTAGTGACGGCTCTGCTGTGGATGTTCACATCAACATGGAACAGGCCCCGATTCAGAGTGAGCCCCGGGTACGGCTGGTGATGGCTCAGCACATGATCAGGGATATACAGACCTTACTATCCCGGATGGAGACTCTCCCCTACCTTCAGTGTCGAGGAGGGCCCCAACCGCAGCACAGTCAGCCGCCCCCGCAGCCACCGGCTGTGACCCCGGAGCCAGTAGCCTTGAGCTCTCAAACATCAGAACCAGTTGAAAGTGAAGCACCTCCCCGGGAGCCCATGGAGGCAGAAGAAGTGGAGGAGCGTGCCCCAGCCCAGAACCCGGAGCTCACTCCTGGCCCAGCCCCAGCGGGCCCAACACCTGCCCCGGAAACAAATGCACCCAACCATCCTTCCCCTGCGGAGTATGTCGAGGTGCTCCAGGAGCTACAGCGGCTGGAGAGTCGCCTCCAGCCCTTCTTGCAGCGCTACTACGAGGTTCTGGGTGCTGCTGCCACCACGGACTACAATAACAATCACGAGGGCCGGGAGGAGGATCAGCGGTTGATCAACTTGGTAGGGGAGAGCCTGCGACTGCTGGGCAACACCTTTGTTGCACTGTCTGACCTGCGCTGCAATCTGGCCTGCACGCCCCCACGACACCTGCATGTGGTCCGGCCTATGTCTCACTACACCACCCCCATGGTGCTCCAGCAGGCAGCCATTCCCATACAGATCAATGTGGGAACCACTGTGACCATGACAGGAAATGGGACTCGGCCCCCCCCAACTCCCAATGCAGAGGCACCTCCCCCTGGTCCTGGGCAGGCCTCATCCGTGGCTCCGTCTTCTACCAATGTCGAGTCCTCAGCTGAGGGGGCTCCCCCGCCAGGTCCAGCTCCCCCGCCAGCCACCAGCCACCCGAGGGTCATCCGGATTTCCCACCAGAGTGTGGAACCCGTGGTCATGATGCACATGAACATTCAAGATTCTGGCACACAGCCTGGTGGTGTTCCGAGTGCTCCCACTGGCCCCCTGGGACCCCCTGGTCATGGCCAAACCCTGGGACAGCAGGTGCCAGGCTTCCCAACAGCTCCAACCCGGGTGGTGATTGCCCGGCCCACTCCTCCACAGGCTCGGCCTTCCCATCCTGGAGGGCCCCCAGTCTCTGGGACACTGCAGGGCGCCGGTCTGGGTACCAATGCCTCGTTGGCCCAGATGGTGAGCGGCCTTGTGGGGCAGCTTCTTATGCAGCCAGTCCTTGTGGCTCAGGGGACCCCAGGTATGGCTCCACCGCCAGCCCCTGCCACTGCTTCTGCCAGTGCTGGCACCACCAACACAGCTACCACAGCTGGCCCCGCTCCTGGGGGGCCTGCCCAGCCTCCACCCACCCCTCAACCCTCCATGGCTGATCTTCAGTTCTCTCAGCTTCTGGGGAACCTGCTAGGGCCTGCAGGGCCAGGGGCTGGAGGGTCTGGTGTGGCTTCTCCCACCATCACTGTGGCGATGCCTGGTGTCCCTGCCTTTCTCCAAGGCATGACTGACTTCTTGCAGGCAACACAGACAGCCCCTCCACCACCCCCACCTCCTCCACCCCCACCACCTGCCCCAGAGCAGCAGACCATGCCCCCACCAGGCTCCCCTTCTGGTGGCGCAGGGAGTCCTGGAGGCCTGGGTCTTGAGAGCCTGTCACCGGAGTTTTTTACCTCAGTGGTGCAGGGTGTGCTCAGCTCCCTGCTGGGCTCCCTGGGGGCTCGGGCTGGCAGCAGTGAAAGTATTGCTGCCTTCATACAACGCCTCAGTGGATCCAGCAACATCTTTGAGCCTGGAGCTGATGGGGCCCTTGGATTCTTTGGGGCCTTGCTTTCTCTTCTGTGCCAGAACTTCTCTATGGTGGACGTAGTGATGCTTCTCCATGGGCATTTCCAGCCACTACAACGGCTCCAGCCCCAGCTGCGATCCTTCTTCCACCAGCACTACCTGGGTGGTCAGGAGCCCACACCCAGTAACATCCGGATGGCAACCCACACATTGATCACGGGGCTAGAAGAGTATGTGCGGGAGAGTTTTTCCTTGGTGCAGGTTCAGCCAGGTGTGGACATCATCCGGACAAACCTGGAATTTCTCCAAGAGCAGTTTAATAGCATTGCTGCGCATGTGCTGCATTGCACAGATAGTGGATTTGGGGCCCGGTTGCTGGAGTTGTGTAACCAAGGCCTGTTTGAATGCCTGGCCCTAAACCTGCACTGCTTGGGGGGACAGCAGATGGAGCTTGCTGCTGTTATCAATGGCCGAATTCGTCGTATGTCTCGTGGGGTGAATCCCTCCTTGGTGAGCTGGCTGACCACTATGATGGGACTGAGGCTTCAGGTGGTACTGGAGCACATGCCTGTAGGCCCTGATGCCATTCTCAGATACGTTCGCAGGGTTGGTGATCCCCCCCAGCCACTTCCTGAGGAGCCAATGGAAGTTCAGGGAGCAGAAAGAGCTTCCCCTGAGCCTCAGCGGGAGAATGCTTCCCCAGCCCCTGGAACAACAGCAGAAGAGGCCATGTCCCGAGGTCCACCTCCTGCTCCTGAGGGGGGCTCCCGGGATGAACAGGATGGAGCTTCAGCTGAGACAGAACCTTGGGCAGCTGCAGTCCCCCCAGAATGGGTCCCTATTATCCAGCAGGACATTCAGAGCCAGCGGAAGGTGAAACCGCAGCCCCCTCTGAGTGATGCCTACCTCAGTGGTATGCCTGCCAAGAGACGCAAGACGATGCAGGGTGAGGGCCCCCAGCTGCTTCTCTCAGAGGCTGTGAGCCGGGCAGCTAAGGCAGCCGGAGCTCGGCCCCTGACGAGCCCCGAGAGCCTGAGCCGGGACCTGGAGGCACCAGAGGTTCAGGAGAGCTACAGGCAGCAGCTCCGGTCTGATATACAAAAACGACTGCAGGAAGACCCCAACTACAGTCCCCAGCGCTTCCCCAATGCCCAGCGGGCCTTTGCTGATGATCCTTAG

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGSGEFGVDMEPNDSTSTAVEEPDSLEVLVKTLDSQTRTFIVGAQMNVKEFKEHIAASVSIPSEKQRLIYQGRVLQDDKKLQEYNVGGKVIHLVERAPPQTHLPSGASSGTGSASATHGGGSPPGTRGPGASVHDRNANSYVMVGTFNLPSDGSAVDVHINMEQAPIQSEPRVRLVMAQHMIRDIQTLLSRMETLPYLQCRGGPQPQHSQPPPQPPAVTPEPVALSSQTSEPVESEAPPREPMEAEEVEERAPAQNPELTPGPAPAGPTPAPETNAPNHPSPAEYVEVLQELQRLESRLQPFLQRYYEVLGAAATTDYNNNHEGREEDQRLINLVGESLRLLGNTFVALSDLRCNLACTPPRHLHVVRPMSHYTTPMVLQQAAIPIQINVGTTVTMTGNGTRPPPTPNAEAPPPGPGQASSVAPSSTNVESSAEGAPPPGPAPPPATSHPRVIRISHQSVEPVVMMHMNIQDSGTQPGGVPSAPTGPLGPPGHGQTLGQQVPGFPTAPTRVVIARPTPPQARPSHPGGPPVSGTLQGAGLGTNASLAQMVSGLVGQLLMQPVLVAQGTPGMAPPPAPATASASAGTTNTATTAGPAPGGPAQPPPTPQPSMADLQFSQLLGNLLGPAGPGAGGSGVASPTITVAMPGVPAFLQGMTDFLQATQTAPPPPPPPPPPPPAPEQQTMPPPGSPSGGAGSPGGLGLESLSPEFFTSVVQGVLSSLLGSLGARAGSSESIAAFIQRLSGSSNIFEPGADGALGFFGALLSLLCQNFSMVDVVMLLHGHFQPLQRLQPQLRSFFHQHYLGGQEPTPSNIRMATHTLITGLEEYVRESFSLVQVQPGVDIIRTNLEFLQEQFNSIAAHVLHCTDSGFGARLLELCNQGLFECLALNLHCLGGQQMELAAVINGRIRRMSRGVNPSLVSWLTTMMGLRLQVVLEHMPVGPDAILRYVRRVGDPPQPLPEEPMEVQGAERASPEPQRENASPAPGTTAEEAMSRGPPPAPEGGSRDEQDGASAETEPWAAAVPPEWVPIIQQDIQSQRKVKPQPPLSDAYLSGMPAKRRKTMQGEGPQLLLSEAVSRAAKAAGARPLTSPESLSRDLEAPEVQESYRQQLRSDIQKRLQEDPNYSPQRFPNAQRAFADDP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU25609
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Needs linker deleted