cDNA Clone FAN1 (MTMR15) (Mouse)

• Plasmid: pBabe.puro.GFP.DU mouse Fan1 L480P (MTMR15)
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP mouse FAN1 L480P
• Genbank: NM_177893.3 a414g silent
• Tag: GFP
• Species: Mouse

Sequence of Insert

ggatccATGCCGTCACAAAGGAAATCACCTGATCAAAAAAGACCCCGTAGAAGCTT
ATCTACCAGCAAAACTGCAAAAAGCCAGTGTCATTCGATTACTTCGTATT
TTAACAGTGCACCACCTGCTAAACTTGCATGTTCAACTTGTCATAAAATG
GTGCCCAGGTATGACCTCATTCGGCACCTTGATGAGTCTTGTGCTAACAA
CGGTGTTGGTGATGACGTTCAAGTAGAGCCTGCTCAGGCTGGATTAATGA
GTCCAACTGTGCCTACATCAGATTTACCCAGTGGTCCTTTAGAGAATGTG
ACACCTCAGAAATTATCACCACCAAAGAGAAGTTTAATTTCTGTCCAATG
TGGTTCAAAACTGGGCATACAACAGCAGACCAGTCCCTACTTTAAAGATG
CCTTAGTGTCCAAGGATCAAAATGAGCTTCCAAATCAGAGTGTAGAAATT
ATGCCTTTGGGAAGTCTGACATCGAAATTGTCCAGACGATACTTAAACGC
TAAAAAATCACTCGCTAAGAATGAGGGACTAGCCAGTCAGTGCCCACAGA
CTTCTCCGTCCACACCTGGCACCAGCCTGACTGATAACTGTCCAGAGATG
GAAGACAAAGATGAGGTTTTGAACAGTTCCCAAAAGGAAAACATTTATTC
ATGTGCACCTCTAAAAGAAGAGAATGCTTCAGAACAGAAGGTAAAGAACA
ATAAAATAACGGGAGATGAAAGCCAGAAAGCTTCCTGCGGGGAGCCAGCC
CTCACCCCTGCATCTGCAGAGCATGCTTCCATATTACTGTCCTCAGATTC
AACTCTCGTCAGTAACACGAAATCCTCTCCGGGAGACACTCTTGTAAAGC
AAGAAAGTGCCAGAAGGGCAGATGTAGGGCTTGCTGAGCCGCTCGAGGTG
CGCAGTCACAAAGAAGTACAAATGACTTTCGATGCTGCGGCCAAAACGCT
GGTGTCTGGGGAGGCAGAATCAAATGGTCCTACCGATGTTGACATGTCTG
ACATGACTACGTGGAGTAACAACCAAGAGCTTGTCAGGGAAGCTGGCAGT
GTCTTACACTGCCCTCTGGAGCAGGGGTCCAGCTGCGGTGGCCCCAGTGA
GACAGCTCAACTGGCACTGAGTCACCCGTACTACCTGCGGAGCTTCCTGG
TGGTGCTGCAGGCCCTCCTTGGGAACGAAGAGGACATGAAGCTCTTCGAT
GAGCAGGAGAAGGCGATTATCACCAGGTTTTACCAGTTGTCAGCTAGTGG
TCAAAAGTTGTATGTGAGGCTCTTTCAACGTAAATTAACCTGGATTAAAA
TGAGTAAACTGGAGTATGAAGAGATTGCCTCCGACTTAACCCCTGTGGTT
GAAGAATTGAAGGACTCAGGCTTTCTACAGACAGAATCTGAGTTGCAAGA
ACTCTCTGATGTACTTGAACTCCTTTCTGCTCCTGAGCcGAAAGCCCTGG
CCAAAACCTTCCACTTGGTGAGTCCCGGTGGGCAGAAGCAGCAGCTGGTA
GACGCCTTTCACAAACTGGCCAAACAGCGCTCAGTCTGCACGTGGGGCAA
GACTCAGCCTGGAATCCGAGCAGTGATTTTAAAAAGAGCCAAAGACTTGG
CTGGCCGGTCGCTTCGAGTCTGTAAAGGCCCTAGGGCTGTGTTTGCCCGA
ATCTTGCTTCTGTTCTCATTGACTGATTCCATGGAAGACGAAGAAGCTGC
TTGTGGGGGTCAAGGTCAGCTGTCTACTGTGCTATTGGTCAATCTGGGCC
GAATGGAGTTTCCTCAGTACACCATCTGCCGGAAGACCCAGATCTTCAGG
GACAGAGAGGACCTCATCAGATACGCAGCAGCCGCACACATGCTGAGTGA
CATCTCAGCTGCCATGGCCAGTGGGAACTGGGAGGACGCTAAGGAGCTTG
CTCGGAGTGCAAAAAGGGACTGGGAACAACTGAAAAGCCACCCTTCCCTG
AGGTACCACGAGGCCTTGCCGCCCTTCCTGCGCTGCTTTACCGTCGGGTG
GATCTACACAAGGATTTCCTCTCGGGCTGTTGAAGTCCTAGAGAGGCTTC
ACATGTACGAGGAAGCTGTCAAGGAACTTGAAAACCTCTTGTCTCAGAAA
ATCTATTGTCCTGACAGCAGAGGCCGCTGGTGGGATCGCCTGGCTCTTAA
CCTGCACCAGCACCTGAAGCGTCTGGAAGAGGCAATTAGGTGCATCAGGG
AAGGCCTGGCAGACCCACACGTGAGGACAGGACACCGACTTTCTCTTTAT
CAGCGAGCCGTGCGCCTGCGAGAGTCTCCGAGCTGCAGGAAGTACAAGCA
CCTCTTCAGCCGGCTGCCCGAGGTTGCCGTGGGAGACGTCAAGCATGTGA
CCATCACGGGCAGGCTGTGCCCACAGCATGGCATGGGCAAGTCTGTGTTT
GTCATGGAGAGTGGAGACGGCGCCAACCCCACCACAGTCCTGTGTTCTGT
GGAGGAGCTGGCCCTGGGTTATTACAGGCAGAGTGGCTTTGACCAAGGGA
TTCATGGAGAAGGGTCCACCTTCAGCACCCTGTGTGGCCTCCTGCTGTGG
GATATCATCTTCATGGATGGAATACCAGACGTCTTCAGAAATGCCTACCA
GGCATCCCCACTGGATTTGCTCACGGACAGCTTCTTCGCAAGCAGGGAGC
AGGCCCTGGAAGCCAGGCTGCAGCTGATCCACAGCGCCCCTGCTGAGAGC
CTGCGGGCCTGGGTGGGCGAAGCATGGCAGGCCCAGCAAGGCAGAGTGGC
CTCTCTGGTCAGCTGGGACCGCTTCACCTCCCTACAGCAAGCTCAGGATC
TTGTCTCCTGCCTCGGGGGTCCTGTCCTCAGTGGTGTGTGCAGGCGCCTG
GCTGCTGACTTTCGGCACTGCCGAGGGGGCCTCCCAGACTTGGTGGTGTG
GAATTCTCAGAGCCACCATTGCAAGCTGGTGGAGGTGAAAGGCCCCAGTG
ATCGACTTTCATGTAAGCAGATGATCTGGCTGTACGAACTCCAAAAGCTG
GGGGCTGATGTAGAAGTGTGCCATGTGGTTGCAGTTGGTGCTAAGAGCAA
AGGTCTTGGCTGAGCGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMPSQR
KSPDQKRPRRSLSTSKTAKSQCHSITSYFNSAPPAKLACSTCHKMVPRYD
LIRHLDESCANNGVGDDVQVEPAQAGLMSPTVPTSDLPSGPLENVTPQKL
SPPKRSLISVQCGSKLGIQQQTSPYFKDALVSKDQNELPNQSVEIMPLGS
LTSKLSRRYLNAKKSLAKNEGLASQCPQTSPSTPGTSLTDNCPEMEDKDE
VLNSSQKENIYSCAPLKEENASEQKVKNNKITGDESQKASCGEPALTPAS
AEHASILLSSDSTLVSNTKSSPGDTLVKQESARRADVGLAEPLEVRSHKE
VQMTFDAAAKTLVSGEAESNGPTDVDMSDMTTWSNNQELVREAGSVLHCP
LEQGSSCGGPSETAQLALSHPYYLRSFLVVLQALLGNEEDMKLFDEQEKA
IITRFYQLSASGQKLYVRLFQRKLTWIKMSKLEYEEIASDLTPVVEELKD
SGFLQTESELQELSDVLELLSAPEPKALAKTFHLVSPGGQKQQLVDAFHK
LAKQRSVCTWGKTQPGIRAVILKRAKDLAGRSLRVCKGPRAVFARILLLF
SLTDSMEDEEAACGGQGQLSTVLLVNLGRMEFPQYTICRKTQIFRDREDL
IRYAAAAHMLSDISAAMASGNWEDAKELARSAKRDWEQLKSHPSLRYHEA
LPPFLRCFTVGWIYTRISSRAVEVLERLHMYEEAVKELENLLSQKIYCPD
SRGRWWDRLALNLHQHLKRLEEAIRCIREGLADPHVRTGHRLSLYQRAVR
LRESPSCRKYKHLFSRLPEVAVGDVKHVTITGRLCPQHGMGKSVFVMESG
DGANPTTVLCSVEELALGYYRQSGFDQGIHGEGSTFSTLCGLLLWDIIFM
DGIPDVFRNAYQASPLDLLTDSFFASREQALEARLQLIHSAPAESLRAWV
GEAWQAQQGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRRLAADFR
HCRGGLPDLVVWNSQSHHCKLVEVKGPSDRLSCKQMIWLYELQKLGADVE
VCHVVAVGAKSKGLG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24427
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.