cDNA Clone HTT

• Synonyms: HD; IT15; huntingtin
• Plasmid: pcDNA5 FRT/TO HTT Q25 GFP
• Parent Plasmid: pcDNA5 FRT/TO C-GFP
• Expressed: HTT Q25 GFP
• Genbank: NM_002111.7
• Tag: GFP
• Species: Human

Sequence of Insert

ATGGCGACCCTGGAAAAGCTGATGAAGGCCTTCGAGTCCCTCAAAAGCTTCCAACAGCAGCAACAGCAACAACAGCAGCAACAGCAACAACAGCAGCAACAGCAACAACAGCAGCAACAGCAACAACCGCCACCACCTCCCCCTCCACCCCCACCTCCTCAACTTCCTCAACCTCCTCCACAGGCACAGCCTCTGCTGCCTCAGCCACAACCTCCTCCACCTCCACCTCCACCTCCTCCAGGCCCAGCTGTGGCTGAGGAGCCTCTGCACCGACCTGGATCCCTG

Amino Acid Sequence

MATLEKLMKAFESLKSFQQQQQQQQQQQQQQQQQQQQQQQQQPPPPPPPPPPPQLPQPPPQAQPLLPQPQPPPPPPPPPPGPAVAEEPLHRPGSLVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU25503
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Clone brought down by John Bett. First Exon of HTT with 25 Glutamines, SDM'd in, no usable restriction sites