cDNA Clone Fancd2 (Xenopus)

• Synonyms: xfancd2
• Plasmid: pFB1 FLAG xenopus Fancd2
• Parent Plasmid: pFastbac1
• Expressed: FLAG xenopus Fancd2
• Genbank: NM_001095691.1 GI:148234373 with changes see Notes
• Tag: FLAG
• Species: Xenopus laevis

Sequence of Insert

GAATTCGCCACCATGGACTACAAGGACGACGATGACAAGATGGTGGCCAAAAGAAAGCTGTCTCGATCAGATGACAGAGAAGAGAGTTTTACTGCAGACACTTCCAAAAACAAGAAGTGTCGTACGAGCAGTAAAAAGTCAAAGGCCTTGCCCCAAGATGGCGTGGTGGAGAATGACAGCATATTCGTGCAGCTCCTAAAGTCATCAGGAATGACTCTCAAATGTGGAGATCGTCAGAATGAAATTGCTGTGGATCAGGCTGTTTTCCAAAGAAAGCTTCAACAGGCTCTAAGAAAGCATCCCAGGCACCCAAATGTCATTCAAGAGTTCATCAGTGGACTAGAATCACACATTGAAGATCGAGATATATTTAGAAATTGCCTTCTTCCCTGTGGAAACAGACAAGAGACAGAAGCAAGTACCATGACTGGTTCCTTCCATGACAGCCTAATAAAACTCCTGTTGGGTACAGATATTTTACAGGAATCTGTTATAAATACATTGTTTGAGAAACTTCCAGAGTTTTTATATGATAGTGTTGGCAGCGATGGAATCAGCATTCCTCGATTAATCATCAACCAATTTAAATGGCTTGACCGCATACTGGATTGCAAGGACCTCACTCTAAAGATTATGCAATTGATCTCTGTGGCACCTGTTGATATTCAGCATGACATTGTCACTAGCTTGCCTGAAATCCTGGAGGATTCTCAACATAACGATGTTGCAAGAGAACTCAACTCTCTGTTGCAACAGAATACACAGCTTACAGTGCCAATTCTAGATGCCTTATCCAGCCTCAATATTAATGCAGACCTGCTTTCTGAGGTGCGCCAGTCAGTTATGTCCACCCTCTCAGCAGTTGAACTAGAGGATTTGCCTGTAATAATAAAGTTTATTCTCCATGCTGTAACACCTTCAGATGCAGTAGAGGTGATTTCTGAGCTCAGAAAGAAACTTGACCTGGAGTCCTGTTCTTCGCTTGAACAGATATATGCAACTCAGAGTAAAGAAAGAAATAAGCCACATGCTGGCTCTTCAGTGAATAAAACAAAGAGCAGTGACTGTGTCTCGCTTATGATGGATGTCATTAAATCGTCTGTCCGATTTCAAAAGCACACGTCTGAAGCTTGGATGAAGGCAATAGAAAATGTTGACACTGTTGGAGACCATAAGGTTTCTGATTTGATTGTTCTCCTGATCCTTTACACCACACAAACAAACAGCAGCAAGAAACAAGCAGAAAGGGTTCTGAGGAACAAGATTCGTTCTGGCTTCATTTTGGACCAGCTATTACAGAATGCCTTCAGGAATCATTCACAGGTTTTGCGAGACTATTTTCCCTCTATTCTGTCCCTGGCCCAATCTATGCTACGATCCGCAGAGCAGAGTGTGGTCTCATTTGGCAGCCTTATGTATAAAAGTGCTTTCTCTTCCTTTGATTCTTACTGCCAGCAGGAGGTTGTTGGAGCATTGGTGACCCATGTGTGCAGTGGTTATGCAGCTGAAGTTGATGTTTCACTTGATGTGCTTACTGATTTGGTGTCCAGTCATGCTGCTGCAGTTGCTCTTTATGCTGTTTTTGTAAAGGGTATATTAGATTACCTGGATAACCTAAATGCACAACAAATCCGTAAACTGTTCCACATCCTCAGTGTACTTGCTTTCAGCAGGGGCCAGGAGGGAGGCCACATTCAGGATGACATGTTTATTGTGATCAGGAAGCAGCTGTCCAGCACAGTCCTTAAGTACAAACGAATTGGTATTATTGGAGCTGTGCAAATGGTTGGAAGTATGGCAATGAACAAAAAACACGCGAGTAAACATCCAGAAAACAAACCTCTAAGTGCAGAAACCTTCAGACAGGTGACAGCCCTTCTTGAGTTGCTACAGACTTGCAGTGAGCGGGTAGCGGAAGCCTCTGCCCTCTATTATGATGAACTGTCCAGCCTGGTGCAGAAGAGAAATCTGGATCCACAAGTCATGAGTTTGGTGGGGAAGACTGTGCTAACAGACTTTCAGGATGACTTTGTGGAGGATCTCACACCAACTGAGGAAGGAAACTACATTTTCCCACTGAAAGCCATGTACAATCTAGATGAGGATGATAGCCAGGGTGGGATTGCTATCAACCTACTTCCTCTTCTGTCCCAGGACATGCGCAATAGAGGTGCAGAACAAGTAGCCAATAAAGAAGGAAGGGTTGTTTCTCCGATCTGTCTGTCTCCATTTTTTCGTCTTCTGAGGCTATGTATAGAAGATCAACATGAAGGCAATCTGGAGGAAATCGATGCATTATTAGGCTGTCCCTTATACCTAACAGACCTAGAGATAACAGAAAAGATGGAGTCTCTGTCCAAACAAGAAAGGGAATTTCTCTGCTCGCTATTGTTCTTTGCTCTTAATTGGTTCAGAGAAATTGTGAATGCGTTCTGCAAGCAGCAAGATCCAGATATGAAGGGCAAAGTGCTGACACGATTACAAAACATCACTTGGCTGCAGTCCGTTCTGGAGAAATGTCTGGCAGCCTCTCCAGGGTACATCCCATCATCTGCACACTTTGATTCTGAGCCACAGGAGGTATTGCCTTCTGCTATAGCACCAGCCCCGGCAAAAAAGGCAAAAAAAGGCAAAACCCCAAAATCAGCAGGGAGTAAAAATGCATCTGCTGACAGCTCTCAGCTAGAGGAACACATGGATACTGAGAAGTCTGAATTGGAAAAAACTCAGCCAGAGAAGGAGAAGGAAGAAAGCAAACCCTCCATCAACCTAAACAACTACCGTGCCTATTTTCGTGAGTTGGATCTGGAAGTCTTCACTGTTCTACAGTGTGGCCTACTTACGCGATCCCTTCTGGACAGTGAAATGCATACAAAGGCAACTGAAGTGGTGCAGTTAGGTCCAGCTGAACTGGTGTTTCTCTTGGAGGATCTGTTTCGAAAGACTGAAAACATACTCACTTCTGCTGCAAAAAGAGTGACGTTCTTAAAGGTGAAAAAAGGCAGAAATTTAGGATTTTCACAGCTGCTCCAAAAAACTCCACAGGAAGTTGCTCAAGCAGTTATCCATCTGTTAAATTCACTGTGCAACCACATTGAGAACATGCACAACTACTTCCAGACACTGATGTTAGAAAACCATGGTGTAGTAGATGCCCCGGGTGTTGATATTAAAGAGCATCAGTATATGTCAACTTGTTACCAGCTGCTGCTCCAAGTTTTCCACACACTCTTTTGTTGGAATGGATTTTCCCAGCATGAAAACAGGAAATTACTGAAGTCTGCACTTATTGCCTTGGCAGGCAGACTGAAAGAGACTGAAACCGAACCTGCCCTTGATGATCTTGTAAGACAGAGTTTCAACTACTTAAAGAATCTACACAGCAGTGTACCTACCTGCTCGTCTGCATTGTGCCTTACTCAGCTGCTGATTGTTATTGCAGAGAAGACAAACGTTCTTCAATATCGGGAGCAGATCGCTTCAATGGCAAAACAATTTCTCTGTCAAGCATGGATCCAACCCAGCGGTGAAAGGGAGAAGGGTATCCGTTACCATGAGAATCTGCAGAGTCTTTTGTGTATATACCTAGAAAATACAGATGATGTCCTAAAAGCTGTTGAGGATATAGCTGGAGTTGGGGTACCAGAGTTGGTCAATGCAGCTAAGGATGCAGCCTCATCTTCCTACCCTACACTCACCAGGCAAACTTTTGTAGTCTTTTTCCGGGTAATGATGGACAAACTGGAGAAGTGTGTGAAGTCTATACCAAATAGCAAGAAAGCTGAAACGCTACAGGAGCAGACTGAGCAGCTTCTAAGTTGGAATCTGGCTGTCAGAGACTTCCACATCCTTGTTAACTTGGTTAAGGTGTTTGATAGCAGGCCTGTACTGAGCATATGCTTAAAGTATGGCCGCCTGTTTGTTGAAACGTTCCTGAAACTTGGAATGCCCCTCTTAGACTGCTGCTTCAAGAAACAGCGGGAGGATGTACAGAGCTTGCTGAAAACCTTACAGCTTAGCACAAGGCAACTTCACCATATGTGTGGCCACTCTAAGATAAACCAAGACACTGCACTTACCAACCATGTACCTCTGCTGAAGAAAACCTTGGAGTTGTTTGTGTATAGAGTAAAGGCGATGCTTGTCTTAAACAACTGCCAGGAGGCTTTCTGGCTAGGAAATCTCAAAAACAGGGACTTGCAGGGAGAAGAGATTGTATCTCAGGTCTCTCAAGAGAGCGAAGCTGAGGAGGAACAGGAATCCCAGCTCCCACCAGAGGAAGAGGAAGCAGAGGAGGAGGAAGAGAATGAAGGAAGTGACAAAGAAATTGAAGGAGGAGATGAGGATAATGAGGATGAAGATTCAGATTAACTCGAG

Amino Acid Sequence

MDYKDDDDKMVAKRKLSRSDDREESFTADTSKNKKCRTSSKKSKALPQDG
VVENDSIFVQLLKSSGMTLKCGDRQNEIAVDQAVFQRKLQQALRKHPRHP
NVIQEFISGLESHIEDRDIFRNCLLPCGNRQETEASTMTGSFHDSLIKLL
LGTDILQESVINTLFEKLPEFLYDSVGSDGISIPRLIINQFKWLDRILDC
KDLTLKIMQLISVAPVDIQHDIVTSLPEILEDSQHNDVARELNSLLQQNT
QLTVPILDALSSLNINADLLSEVRQSVMSTLSAVELEDLPVIIKFILHAV
TPSDAVEVISELRKKLDLESCSSLEQIYATQSKERNKPHAGSSVNKTKSS
DCVSLMMDVIKSSVRFQKHTSEAWMKAIENVDTVGDHKVSDLIVLLILYT
TQTNSSKKQAERVLRNKIRSGFILDQLLQNAFRNHSQVLRDYFPSILSLA
QSMLRSAEQSVVSFGSLMYKSAFSSFDSYCQQEVVGALVTHVCSGYAAEV
DVSLDVLTDLVSSHAAAVALYAVFVKGILDYLDNLNAQQIRKLFHILSVL
AFSRGQEGGHIQDDMFIVIRKQLSSTVLKYKRIGIIGAVQMVGSMAMNKK
HASKHPENKPLSAETFRQVTALLELLQTCSERVAEASALYYDELSSLVQK
RNLDPQVMSLVGKTVLTDFQDDFVEDLTPTEEGNYIFPLKAMYNLDEDDS
QGGIAINLLPLLSQDMRNRGAEQVANKEGRVVSPICLSPFFRLLRLCIED
QHEGNLEEIDALLGCPLYLTDLEITEKMESLSKQEREFLCSLLFFALNWF
REIVNAFCKQQDPDMKGKVLTRLQNITWLQSVLEKCLAASPGYIPSSAHF
DSEPQEVLPSAIAPAPAKKAKKGKTPKSAGSKNASADSSQLEEHMDTEKS
ELEKTQPEKEKEESKPSINLNNYRAYFRELDLEVFTVLQCGLLTRSLLDS
EMHTKATEVVQLGPAELVFLLEDLFRKTENILTSAAKRVTFLKVKKGRNL
GFSQLLQKTPQEVAQAVIHLLNSLCNHIENMHNYFQTLMLENHGVVDAPG
VDIKEHQYMSTCYQLLLQVFHTLFCWNGFSQHENRKLLKSALIALAGRLK
ETETEPALDDLVRQSFNYLKNLHSSVPTCSSALCLTQLLIVIAEKTNVLQ
YREQIASMAKQFLCQAWIQPSGEREKGIRYHENLQSLLCIYLENTDDVLK
AVEDIAGVGVPELVNAAKDAASSSYPTLTRQTFVVFFRVMMDKLEKCVKS
IPNSKKAETLQEQTEQLLSWNLAVRDFHILVNLVKVFDSRPVLSICLKYG
RLFVETFLKLGMPLLDCCFKKQREDVQSLLKTLQLSTRQLHHMCGHSKIN
QDTALTNHVPLLKKTLELFVYRVKAMLVLNNCQEAFWLGNLKNRDLQGEE
IVSQVSQESEAEEEQESQLPPEEEEAEEEEENEGSDKEIEGGDEDNEDED
SD*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU49470
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Difference compared with NM_001095691.1 GI:148234373 CDS:; a198g (silent); t889g (L297V) (common variant); c1728t (silent); g1778g (G593A) (common variant); t3486c (silent); g3972a (silent)
• Low Temp: No