cDNA Clone PSF1 (C. elegans)

• Plasmid: pET15b 6His 3C c. elegans Psf1 M1-H201(end)
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His 3C c. elegans Psf1 M1-H201(end)
• Genbank: NM_063752.4 GI:392891373
• Tag: 6His
• Species: Caenorhabditis elegans

Sequence of Insert

GGATCCATGAGTTCCGGCGATCAGAATCGAGGAGGCGTCGCCGACAAAGCACTACAACTTGTTTTAGAGATGAAAAGAAATCCAGATGTATTACCGCCTTATAACACAGAACTTGTACGCCAATGTTATCAGAAAATCGATGAGTTGTTTCAAAAGAATGCTGCCGTCGTTGAAAAAATCCGTGCAGGACTCCCTCATGATTCTACGCTTCTTCAACCTCGTTTAGCTGCAATGTGCCATATTCGACGTTGCATGATGGCCTATGTGAACGAGAGGAAAAACAGGATTCGATCATTCAGATGGAAGTATGGAGGTGCTCTTCCCGCGTCTGTTAGGAACGCCCTCTGCGATGCGGAAATTCAGTTTTTCAATGAGTATTCGAGCACATTGGCAAGATTCCAAAGCAATCTTGGAGAAGGTGGTGTTAATCTGCTGCTTCATTCTGCACCACCGAAGTCACTTTTTGTACAAGTTAGAGCTCTCGAGGATTATGGAGAATTTGAAACGTCAGATGGAACTCAAGTTCAGCTGTCCAAGGATAGTTTGCACTCCCTTCCACGCCAAGACTGCGAAATGCTGATACGTCAGGGTGTTCTCGAGCTTGTTCATTAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMSSGDQNRGGVADKALQLVLEMKRNPD
VLPPYNTELVRQCYQKIDELFQKNAAVVEKIRAGLPHDSTLLQPRLAAMC
HIRRCMMAYVNERKNRIRSFRWKYGGALPASVRNALCDAEIQFFNEYSST
LARFQSNLGEGGVNLLLHSAPPKSLFVQVRALEDYGEFETSDGTQVQLSK
DSLHSLPRQDCEMLIRQGVLELVH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU49539
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make a construct to introduce 9His at the N-terminus of C. elegans PSF-1 (1-201), for expression in E. coli. Please then pass this to the protein expression and antibody team. We would like to make affinity purified polyclonal antibodies for this protein in SHEEP.; Remi will be happy to supply either a cDNA library for C. elegans, or to help make RNA if necessary.; ; Here is the sequence of the C. elegans PSF-1 protein:; MSSGDQNRGGVADKALQLVLEMKRNPDVLPPYNTELVRQCYQKIDELFQK; NAAVVEKIRAGLPHDSTLLQPRLAAMCHIRRCMMAYVNERKNRIRSFRWK; YGGALPASVRNALCDAEIQFFNEYSSTLARFQSNLGEGGVNLLLHSAPPK; SLFVQVRALEDYGEFETSDGTQVQLSKDSLHSLPRQDCEMLIRQGVLELV; H; ; Here is the sequence of C. elegans psf-1 cDNA without STOP codon:; ATGAGTTCCGGCGATCAGAATCGAGGAGGCGTCGCCGACAAAGCACTACA; ACTTGTTTTAGAGATGAAAAGAAATCCAGATGTATTACCGCCTTATAACA; CAGAACTTGTACGCCAATGTTATCAGAAAATCGATGAGTTGTTTCAAAAG; AATGCTGCCGTCGTTGAAAAAATCCGTGCAGGACTCCCTCATGATTCTAC; GCTTCTTCAACCTCGTTTAGCTGCAATGTGCCATATTCGACGTTGCATGA; TGGCCTATGTGAACGAGAGGAAAAACAGGATTCGATCATTCAGATGGAAG; TATGGAGGTGCTCTTCCCGCGTCTGTTAGGAACGCCCTCTGCGATGCGGA; AATTCAGTTTTTCAATGAGTATTCGAGCACATTGGCAAGATTCCAAAGCA; ATCTTGGAGAAGGTGGTGTTAATCTGCTGCTTCATTCTGCACCACCGAAG; TCACTTTTTGTACAAGTTAGAGCTCTCGAGGATTATGGAGAATTTGAAAC; GTCAGATGGAACTCAAGTTCAGCTGTCCAAGGATAGTTTGCACTCCCTTC; CACGCCAAGACTGCGAAATGCTGATACGTCAGGGTGTTCTCGAGCTTGTT; CAT