cDNA Clone MCM7 (C. elegans)

• Plasmid: pET15b 6His 3C c. elegans mcm7 M1-K222
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His 3C c. elegans mcm7 M1-K222
• Genbank: NM_071798.5 GI:392918804
• Tag: 6His
• Species: Caenorhabditis elegans

Sequence of Insert

GGATCCATGAAAACCACCACTTACAACACAGACTGGGCTGCCGAGAAGACGAAAATTCGCAGTTTCTTCGACGAATACTATGTGGACAATGAGGACGGATCTGGAAAGGCATTCCCATATCGTGATCAAGTCTTTGAAATTGCTCGCCGCGACAAGCAGGCAATCGTTGTGAATGTTGACCATATTAAGGAATCTGATATTCCAGATGCTCTTGAACTCTCAGAAGCCATTACTTCCAATACAAAGAGATACGAAGTCCTGTTCAAGGACACAATTTCGGATATGATCCAGGACTATCTCGGAGACAAGCAAGCGCCAGTAATCGATGCTCTTGATGCCTACATGTTCCAGCGTCTTCATATGGATAGGAACGAGGGAGCCGCCAACGAGGAAGTTTCTCTTCAGGACAAACGCAAGAAATACCCACCACAGCTCCTTCAACGCTTCGAGGTCTACTTCACAACGGACGATGCCGCCCATGAAACGTGTGTCCGAAACATCAAAGCCACCGAGATCGGGCACTTGGTCTCCATGAAGGGAGTTGTTATCCGTGCGACCGAGGTGAAACCTTGTGTTGAAGTCATGACCTACACTTGCGATACTTGTGCCGCCGAAGTCTATCAGCCTGTGAAGGGAATGCAATTCACCCCACCGGTCAACTGTCCCAACAAGTAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMKTTTYNTDWAAEKTKIRSFFDEYYVD
NEDGSGKAFPYRDQVFEIARRDKQAIVVNVDHIKESDIPDALELSEAITS
NTKRYEVLFKDTISDMIQDYLGDKQAPVIDALDAYMFQRLHMDRNEGAAN
EEVSLQDKRKKYPPQLLQRFEVYFTTDDAAHETCVRNIKATEIGHLVSMK
GVVIRATEVKPCVEVMTYTCDTCAAEVYQPVKGMQFTPPVNCPNK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU49538
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make a construct to introduce 9His at the N-terminus of; C. elegant MCM-7 (1-222), for expression in E. coli. Please then pass this; to the protein expression and antibody team. We would like to make affinity; purified polyclonal antibodies for this protein in SHEEP.; I will be happy to supply either a cDNA library for C. elegans, or to help; make RNA if necessary.; ; Here is the sequence of the C. elegant N-terminus MCM-7 protein:; MKTTTYNTDWAAEKTKIRSFFDEYYVDNEDGSGKAFPYRDQVFEIARRDK; QAIVVNVDHIKESDIPDALELSEAITSNTKRYEVLFKDTISDMIQDYLGD; KQAPVIDALDAYMFQRLHMDRNEGAANEEVSLQDKRKKYPPQLLQRFEVY; FTTDDAAHETCVRNIKATEIGHLVSMKGVVIRATEVKPCVEVMTYTCDTC; AAEVYQPVKGMQFTPPVNCPNK; ; ; Here is the sequence of C. elegant 5' fragment of mcm-7 cDNA (without STOP codon):; ATGAAAACCACCACTTACAACACAGACTGGGCTGCCGAGAAGACGAAAAT; TCGCAGTTTCTTCGACGAATACTATGTGGACAATGAGGACGGATCTGGAA; AGGCATTCCCATATCGTGATCAAGTCTTTGAAATTGCTCGCCGCGACAAG; CAGGCAATCGTTGTGAATGTTGACCATATTAAGGAATCTGATATTCCAGA; TGCTCTTGAACTCTCAGAAGCCATTACTTCCAATACAAAGAGATACGAAG; TCCTGTTCAAGGACACAATTTCGGATATGATCCAGGACTATCTCGGAGAC; AAGCAAGCGCCAGTAATCGATGCTCTTGATGCCTACATGTTCCAGCGTCT; TCATATGGATAGGAACGAGGGAGCCGCCAACGAGGAAGTTTCTCTTCAGG; ACAAACGCAAGAAATACCCACCACAGCTCCTTCAACGCTTCGAGGTCTAC; TTCACAACGGACGATGCCGCCCATGAAACGTGTGTCCGAAACATCAAAGC; CACCGAGATCGGGCACTTGGTCTCCATGAAGGGAGTTGTTATCCGTGCGA; CCGAGGTGAAACCTTGTGTTGAAGTCATGACCTACACTTGCGATACTTGT; GCCGCCGAAGTCTATCAGCCTGTGAAGGGAATGCAATTCACCCCACCGGT; CAACTGTCCCAACAAG