cDNA Clone PSF3 (C. elegans)

• Synonyms: Y65B4BR.8
• Plasmid: pET15b 6His 3C c. elegans Psf3 M1-L193(end)
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His 3C c. elegans Psf3 M1-L193(end)
• Genbank: NM_058347.4 GI:392884650
• Tag: 6His
• Species: Caenorhabditis elegans

Sequence of Insert

GGATCCATGGCTGGATTTGAAATTCAAAGAGTCGAGTCCAAATTCTTCGACTTGGACGACATAATCGCCAAGTCAGACTCCACGTCTTGCACATTTGAGATTGGAGATTTGAATCCAGATTTCTTCCAGGAAATGCTGGGAGTTTCGAAGCCAACTCAAAATGCAGACGGATACGGCGTTGATGCTCCATACTGGCTGCTGGAATCCGTTAGAAGTTCATTTTCCATTCAACTGCCAAAAGCTTATAGTGTGAACATGCAAAATGTGCTGAACGCTGACAGCAAGAAATTGAATCTCTCCGGCTTGCAGCAACACTTTTACGGCAACGGGATGCAGTTATGCCGTCTGATGAAGGGCGAAAATCCGGACGGCGCGCTGAGCCTCGCACGATGCCTTGTCTCCACACTTACACAGCGATTAGGAGAAATTGTGTCAACTGCAACCCATCTGCAAAGCAAAGGCGAGAAATTTGACAGCCTCGAGACAAAAGTGTTCCTGGAGGGTAAACGGTGCAAAGAAGACATTGATACTTGGCTGAGACAAGACAATAAGTGCTCGAGCAAGAAGCGTAAGAGACTTTCGTTATAGCGGCCGC

Amino Acid Sequence

MGSSPHHHHHSSGLEVLFQGPGSMAGFEIQRVESKFFDLDDIIAKSDSTS
CTFEIGDLNPDFFQEMLGVSKPTQNADGYGVDAPYWLLESVRSSFSIQLP
KAYSVNMQNVLNADSKKLNLSGLQQHFYGNGMQLCRLMKGENPDGALSLA
RCLVSTLTQRLGEIVSTATHLQSKGEKFDSLETKVFLEGKRCKEDIDTWL
RQDNKCSSKKRKRLSL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU49541
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make a construct to introduce 6His to the full length of; C. elegant PSF-3 (1-193), for expression in E. coli. Please then pass this; to the protein expression and antibody team. We would like to make affinity; purified polyclonal antibodies for this protein in RABBIT.; I will be happy to supply either a cDNA library for C. elegans, or to help; make RNA if necessary.; ; Here is the sequence of the C. elegant PSF-3 protein:; MAGFEIQRVESKFFDLDDIIAKSDSTSCTFEIGDLNPDFFQEMLGVSKPT; QNADGYGVDAPYWLLESVRSSFSIQLPKAYSVNMQNVLNADSKKLNLSGL; QQHFYGNGMQLCRLMKGENPDGALSLARCLVSTLTQRLGEIVSTATHLQS; KGEKFDSLETKVFLEGKRCKEDIDTWLRQDNKCSSKKRKRLSL; ; Here is the sequence of C. elegant psf-3 cDNA (without STOP; codon):; ATGGCTGGATTTGAAATTCAAAGAGTCGAGTCCAAATTCTTCGACTTGGA; CGACATAATCGCCAAGTCAGACTCCACGTCTTGCACATTTGAGATTGGAG; ATTTGAATCCAGATTTCTTCCAGGAAATGCTGGGAGTTTCGAAGCCAACT; CAAAATGCAGACGGATACGGCGTTGATGCTCCATACTGGCTGCTGGAATC; CGTTAGAAGTTCATTTTCCATTCAACTGCCAAAAGCTTATAGTGTGAACA; TGCAAAATGTGCTGAACGCTGACAGCAAGAAATTGAATCTCTCCGGCTTG; CAGCAACACTTTTACGGCAACGGGATGCAGTTATGCCGTCTGATGAAGGG; CGAAAATCCGGACGGCGCGCTGAGCCTCGCACGATGCCTTGTCTCCACAC; TTACACAGCGATTAGGAGAAATTGTGTCAACTGCAACCCATCTGCAAAGC; AAAGGCGAGAAATTTGACAGCCTCGAGACAAAAGTGTTCCTGGAGGGTAA; ACGGTGCAAAGAAGACATTGATACTTGGCTGAGACAAGACAATAAGTGCT; CGAGCAAGAAGCGTAAGAGACTTTCGTTA