cDNA Clone MCM2 (C. elegans)

• Plasmid: pET15b 6His 3C c. elegans mcm2 M1-Y222
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His 3C c. elegans mcm2 M1-Y222
• Genbank: NM_001027245.5 GI:392891981
• Tag: 6His
• Species: Caenorhabditis elegans

Sequence of Insert

AGATCTATGGCTGATCGTGCTAACAACGATGACGACGTCGATCGCCAGCCACTTCCGATCGCCGACGATGCGGATGATGACGTTGACGGCATCGATGAGATGTTCAACAACGAGGACGAGGATCCAGAGGATGAGGAGGGCGAGAATCTTTTCGGAGATGATATGGAGAGGGACTACCGTGAGCAGCCGGAGCTCGATCAGTACTCCGAATCTGGAATGGACGACGCATCGGACGTCGGAAGTCTCTCGGTGAGCGCCCGCCGTGCCGCCGAACGTGAGATGGCTCAGCGTGATCAACTGCTCGACGACGATGCTCTGATGTACGAGGATGGAGATAGCGAGGAGGTTGACACACGTCGTCGTGGACGTGGACGTCGCGGTCGAGGTGATGCTGCTGATGACGATTCGGTTCCGATGGAGGAAGAGGACATTCCTGTAGATATTCTCGAGAACATCCGCGGTCGAACAATCCGTGATCACGTCTCCGATGAGGCTGTCGCCAAGGAGATTGAGCGAAGATTCAAGAATTTCCTTCGAAGCTTCCACGAACCGGGCAACAAGCAGACCAAGTATATTCAGATGATCAAATCTATGGCGGCGGATAATCGGGAATCGCTGGAAGTCAGTTTTACGGATCTTAGTGATGATAACGGAGAGCAGAACATCTCATACTAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMADRANNDDDVDRQPLPIADDADDDVD
GIDEMFNNEDEDPEDEEGENLFGDDMERDYREQPELDQYSESGMDDASDV
GSLSVSARRAAEREMAQRDQLLDDDALMYEDGDSEEVDTRRRGRGRRGRG
DAADDDSVPMEEEDIPVDILENIRGRTIRDHVSDEAVAKEIERRFKNFLR
SFHEPGNKQTKYIQMIKSMAADNRESLEVSFTDLSDDNGEQNISY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU49542
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make a construct to introduce 6His at the N-terminus of C. elegant MCM-2 (1-222), for expression in E. coli. Please then pass this to the protein expression and antibody team. We would like to make affinity purified polyclonal antibodies for this protein in RABBIT.; I will be happy to supply either a cDNA library for C. elegans, or to help make RNA if necessary.; ; Here is the sequence of the C. elegant N-terminus MCM-2 protein:; MADRANNDDDVDRQPLPIADDADDDVDGIDEMFNNEDEDPEDEEGENLFG; DDMERDYREQPELDQYSESGMDDASDVGSLSVSARRAAEREMAQRDQLLD; DDALMYEDGDSEEVDTRRRGRGRRGRGDAADDDSVPMEEEDIPVDILENI; RGRTIRDHVSDEAVAKEIERRFKNFLRSFHEPGNKQTKYIQMIKSMAADN; RESLEVSFTDLSDDNGEQNISY; ; Here is the sequence of C. elegant 5' fragment of MCM-2 cDNA (without STOP codon):; ATGGCTGATCGTGCTAACAACGATGACGACGTCGATCGCCAGCCACTTCC; GATCGCCGACGATGCGGATGATGACGTTGACGGCATCGATGAGATGTTCA; ACAACGAGGACGAGGATCCAGAGGATGAGGAGGGCGAGAATCTTTTCGGA; GATGATATGGAGAGGGACTACCGTGAGCAGCCGGAGCTCGATCAGTACTC; CGAATCTGGAATGGACGACGCATCGGACGTCGGAAGTCTCTCGGTGAGCG; CCCGCCGTGCCGCCGAACGTGAGATGGCTCAGCGTGATCAACTGCTCGAC; GACGATGCTCTGATGTACGAGGATGGAGATAGCGAGGAGGTTGACACACG; TCGTCGTGGACGTGGACGTCGCGGTCGAGGTGATGCTGCTGATGACGATT; CGGTTCCGATGGAGGAAGAGGACATTCCTGTAGATATTCTCGAGAACATC; CGCGGTCGAACAATCCGTGATCACGTCTCCGATGAGGCTGTCGCCAAGGA; GATTGAGCGAAGATTCAAGAATTTCCTTCGAAGCTTCCACGAACCGGGCA; ACAAGCAGACCAAGTATATTCAGATGATCAAATCTATGGCGGCGGATAAT; CGGGAATCGCTGGAAGTCAGTTTTACGGATCTTAGTGATGATAACGGAGA; GCAGAACATCTCATAC