cDNA Clone TRIM49

• Synonyms: RNF18, TRIM49L2
• Plasmid: pMK-RQ TRIM49 Nter PURO donor
• Parent Plasmid: pMK-RQ
• Expressed: Puro (following recombination)
• Genbank: ENSG00000168930
• Species: Human

Sequence of Insert

CCATGGTGGTTTGCTGCCCGGCTTTTGTGACTCTCTATAAAAATATGTCATAGAACCATCTTCTCATATTTCTTTGGCCATCTTCTCATATTTCTTTGGCCCGGGTAGTTTTTTCCCTCCCACTCTTTCACAGTCATTCATCAGTTATTGAAAGAAGAATAGGGACAACTATGATTTATTCTGACCACTTAGAATCAAAACCCAATTTCCAGATCTATGTTCAGAAAATGGGTAGTTGAATAGGTTTGTATTATGCTACAGACAGGAACTAGGAGTAGAAGGGACTGTGAGTCCTCTGGTTAGTAATATCTGTTAGAAAGCCAGGAGACTCCCTCTAAGTGTAAATTTTTAAGTTTGTAACAGAAGAAATAGTTTGTTGTACTTTAATGAACACTGTCAAGAGAAGAAAATATAACTATCACATATCTCCACATGTTTAGAAGGTTTTCATCAACCCAGGCCCCAAAATGACATGTTTCTCTTTGTTCCTCAGAAACATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGTGCCCGAAGGACCGCGCGACCTGGTGCATGACCCGCAAGCCCGGTGCCTGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGGTAAGTGATGGCTCTGAAGATCGATTTCTGTAAAGGAAGCATAAAATTCCTGTGGGTCTATTTTCTTGGAGATTGGATAAAGCCAACTCTGAGTCCCTTTAAGCAGCTCTGTTTGGGCTTTCTTAGCTTCCAACCTCTGGGCTTTGACAAACATGAAGGGAAACAAAGGAAATGCCATTTACTAGGGGCTTATTTGTCTCTCATTCTGGGCCCCCTCCCTATGTCATGGTCTGCACTGGTGCTTCAATTTATGGCTCTTTTGCAGGAGAAGCTTTTACAGAAAATGCAGTCTTTGTGGGAAAAAGCTTGTGAAAATCACAGAAACCTGAATGTGGAAACCACCAGAACCAGATGCTGGAAGGTTAGTCCTGTACTACTCTACCTTCTCCAGGAACTTATGGTGGGCAAATGGGTGACTCTTAAAATAGGAACTTGATATCAAACTGTAATGTTTCTGGGATACAGTAAAAAAAAAAAGGCGAGAGAAAACACTGAGAAAA

Amino Acid Sequence

MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQ
ELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSG
SRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEA
AERAGVPAFLETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*

• Vector Type: Other
• Antibiotic: Kan
• DU Number: DU48233
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR donor designed to knockout TRIM49 by inserting Puro to the N-terminus (exon 3) via homologous recombination. Consists of a puromycin-polyA cassette flanked on either side by 500 bp of TRIM49 homology and is to be co-transfected with 48096 into a Cas9 expressing cell-line.; Sequence of insert shows the donor sequence whilst sequence of cassette and amino acid sequence depict the expected expression product from the resultant transcript.