cDNA Clone SV2A (mouse)

• Synonyms: mKIAA0736, AI746429
• Plasmid: pCMV mSV2A T84A c-mCerulean shRNAi I res
• Parent Plasmid: pmCerulean N1
• Expressed: SV2A (T84A)-mouse cerulean
• Genbank: NM_022030.3
• Tag: mCerulean
• Species: Mouse

Sequence of Insert

GGATCCATGGAAGAAGGCTTTCGAGACCGAGCAGCGTTCATCCGTGGGGCCAAAGACATTGCCAAGGAAGTGAAGAAGCATGCGGCCAAGAAGGTGGTGAAGGGCCTTGACAGAGTCCAGGATGAGTATTCCCGAAGGTCCTACTCCCGCTTTGAGGAGGAGGACGACGACGATGACTTCCCTGCCCCTGCGGACGGCTATTACCGCGGAGAAGGGGCCCAGGATGAGGAGGAAGGTGGCGCTTCTAGTGATGCCGCCGAGGGCCACGATGAAGATGATGAGATCTATGAGGGAGAATATCAGGGCATCCCCCGGGCAGAGTCTGGGGGCAAAGGCGAACGCATGGCAGATGGGGCACCCCTGGCTGGAGTGAGAGGGGGCTTGAGTGATGGGGAGGGTCCCCCTGGGGGTCGGGGGGAGGCGCAGCGGCGTAAAGATCGGGAAGAGCTTGCGCAGCAATACGAAACCATCCTCCGGGAGTGTGGCCATGGCCGCTTCCAGTGGACACTCTACTTCGTGCTGGGTCTGGCGCTGATGGCAGATGGTGTAGAGGTCTTTGTGGTGGGCTTTGTGCTGCCCAGTGCTGAGAAAGACATGTGCCTGTCGGACTCCAACAAAGGCATGCTAGGCCTCATTGTGTACCTGGGCATGATGGTGGGGGCCTTCCTCTGGGGAGGCCTGGCTGATCGGCTGGGTCGGAGACAGTGTCTGCTCATCTCACTCTCAGTCAACAGCGTCTTCGCCTTCTTCTCGTCCTTCGTCCAGGGTTATGGAACCTTTCTCTTCTGCCGCCTCCTTTCCGGGGTCGGGATTGGCGGTTCCATCCCCATAGTCTTCTCCTATTTTTCGGAGTTTCTGGCGCAGGAGAAACGTGGGGAGCATTTGAGCTGGCTCTGTATGTTCTGGATGATCGGTGGAGTGTATGCAGCTGCAATGGCCTGGGCCATCATCCCTCACTATGGGTGGAGTTTCCAGATGGGCTCTGCTTACCAGTTCCACAGCTGGAGGGTGTTTGTCCTCGTGTGTGCCTTTCCCTCTGTGTTTGCCATCGGGGCTCTGACTACGCAGCCAGAGAGTCCCCGCTTCTTCCTAGAGAATGGGAAGCATGACGAAGCCTGGATGGTACTGAAGCAGGTTCACGACACCAACATGCGAGCCAAGGGCCACCCTGAGCGCGTCTTCTCAGTGACCCACATTAAAACGATTCATCAAGAGGATGAATTGATTGAGATCCAGTCTGACACAGGAACCTGGTACCAGCGCTGGGGAGTACGGGCTTTGAGCCTTGGGGGTCAGGTTTGGGGGAATTTCCTCTCCTGCTTCAGTCCAGAGTATCGGCGCATCACGCTGATGATGATGGGTGTGTGGTTCACCATGTCTTTCAGCTACTATGGTTTGACTGTCTGGTTTCCCGACATGATCCGCCATCTCCAGGCTGTGGACTATGCAGCCCGAACCAAAGTGTTCCCAGGGGAGCGCGTGGAGCATGTGACGTTTAACTTCACACTGGAGAATCAGATCCACCGAGGGGGACAGTACTTCAATGACAAGTTCATCGGGCTGCGTCTGAAGTCAGTGTCCTTTGAGGATTCCCTGTTTGAGGAGTGTTACTTTGAAGATGTTACATCCAGCAACACATTCTTCCGCAACTGCACGTTCATCAACACTGTGTTCTATAACACTGACCTATTTGAGTACAAGTTCGTGAACAGCCGGCTGGTGAACAGCACGTTCCTGCACAATAAGGAAGGCTGCCCGCTAGACGTGACGGGGACAGGCGAAGGTGCCTACATGGTGTACTTTGTCAGCTTCTTGGGGACACTGGCTGTGCTTCCTGGAAACATTGTGTCTGCTCTGCTCATGGACAAGATTGGCAGGCTCAGAATGCTTGCTGGTTCCAGTGTGTTGTCCTGTGTGTCCTGCTTCTTCCTGTCTTTTGGGAACAGCGAGTCAGCCATGATCGCTCTGCTCTGCCTTTTTGGGGGAGTTAGCATCGCATCCTGGAACGCGCTGGACGTGCTGACCGTTGAGCTCTACCCTTCCGACAAGAGGACTACTGCCTTTGGCTTCCTGAATGCCCTGTGTAAGCTGGCAGCTGTGCTGGGGATCAGCATCTTCACATCCTTTGTGGGCATCACCAAGGCTGCTCCCATTCTCTTTGCCTCGGCTGCTCTTGCCCTTGGTAGCTCTCTGGCTCTGAAGCTGCCTGAGACCCGGGGACAGGTGCTGCAG

Amino Acid Sequence

MEEGFRDRAAFIRGAKDIAKEVKKHAAKKVVKGLDRVQDEYSRRSYSRFE
EEDDDDDFPAPADGYYRGEGAQDEEEGGASSDAAEGHDEDDEIYEGEYQG
IPRAESGGKGERMADGAPLAGVRGGLSDGEGPPGGRGEAQRRKDREELAQ
QYETILRECGHGRFQWTLYFVLGLALMADGVEVFVVGFVLPSAEKDMCLS
DSNKGMLGLIVYLGMMVGAFLWGGLADRLGRRQCLLISLSVNSVFAFFSS
FVQGYGTFLFCRLLSGVGIGGSIPIVFSYFSEFLAQEKRGEHLSWLCMFW
MIGGVYAAAMAWAIIPHYGWSFQMGSAYQFHSWRVFVLVCAFPSVFAIGA
LTTQPESPRFFLENGKHDEAWMVLKQVHDTNMRAKGHPERVFSVTHIKTI
HQEDELIEIQSDTGTWYQRWGVRALSLGGQVWGNFLSCFSPEYRRITLMM
MGVWFTMSFSYYGLTVWFPDMIRHLQAVDYAARTKVFPGERVEHVTFNFT
LENQIHRGGQYFNDKFIGLRLKSVSFEDSLFEECYFEDVTSSNTFFRNCT
FINTVFYNTDLFEYKFVNSRLVNSTFLHNKEGCPLDVTGTGEGAYMVYFV
SFLGTLAVLPGNIVSALLMDKIGRLRMLAGSSVLSCVSCFFLSFGNSESA
MIALLCLFGGVSIASWNALDVLTVELYPSDKRTTAFGFLNALCKLAAVLG
ISIFTSFVGITKAAPILFASAALALGSSLALKLPETRGQVLQMVSKGEEL
FTGVVPILVELDGDVNGHKFSVSGEGEGDATLNGKLTLKFICTTGKLPVP
WPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADK
QKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSK
LSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU48237
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: T84A, shRNA 1 resistant.