cDNA Clone Ubiquitin

• Plasmid: pET24a 6His Ubiquitin
• Parent Plasmid: pET24a original
• Expressed: 6His-Ubiquitin
• Genbank: P62988*
• Tag: 6His
• Species: Human

Sequence of Insert

ATGCATCACCATCACCATCACGGCGGTATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGGATCC

Amino Acid Sequence

MHHHHHHGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQ
RLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU49503
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for 6His-tagged ubiquitin (Arg73 codon AGA mutated to more common CGA); ; Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389).