cDNA Clone Rap80/Cezanne

• Synonyms: UIMC1; X2HRIP110; ZA20D1; OTUD7B
• Plasmid: pcDNA5D FRT/TO GFP Rap80 60-124-NLS-Cezanne OTU (OTUD7B 124-438) C194A
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP Rap80 60-124-NLS-Cezanne OTU (OTUD7B 124-438) C194A
• Genbank: Rap80: NM_016290.4, Cezanne: NM_020205.3
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCACGAAGACAAAACAGTCGAATAGAGCAAAGTGTTTGGCCAAAAGAAAAATCGCACAGATGACAGAAGAAGAACAGTTTGCTCTGGCTCTCAAAATGAGTGAGCAGGAAGCTAGGGAGGTGAACAGCCAGGAGGAGGAAGAAGAGGAGCTCTTGAGGAAAGCCATTGCTGAAAGCCTGAATAGTTGCCGGCCTTCTCCACCGAAGAAGAAGCGCAAGGTCAATGAGCACCCCCTGGAAATGCCCATCTGTGCCTTCCAGCTTCCAGATCTCACTGTATACAATGAAGACTTCCGCAGCTTCATAGAGAGAGACCTCATTGAGCAGTCCATGCTGGTTGCCTTGGAACAGGCAGGGCGTTTGAACTGGTGGGTGAGTGTGGACCCCACCTCTCAGAGGCTGCTTCCTTTGGCAACTACTGGAGATGGGAACGCTCTCCTGCATGCAGCCTCCCTTGGAATGTGGGGTTTCCATGATCGGGACTTGATGCTGCGGAAAGCTTTGTATGCACTGATGGAGAAGGGAGTTGAGAAGGAAGCGTTGAAAAGGCGCTGGAGGTGGCAGCAGACACAGCAGAATAAAGAGTCAGGGCTGGTATACACAGAAGATGAATGGCAGAAGGAGTGGAATGAACTGATCAAGCTTGCCTCAAGTGAACCCCGAATGCATCTAGGTACCAATGGAGCCAACTGTGGTGGGGTGGAGAGTTCTGAGGAGCCTGTATATGAGAGCCTTGAAGAGTTTCACGTCTTTGTCCTTGCTCATGTGCTTAGGAGGCCCATAGTCGTCGTGGCAGACACCATGCTGAGGGACTCCGGAGGGGAAGCATTTGCCCCTATTCCCTTTGGAGGAATCTATCTGCCTTTGGAGGTCCCAGCCAGCCAGTGTCACCGCTCCCCTCTGGTGCTCGCCTATGATCAGGCCCACTTTTCTGCACTCGTGTCCATGGAGCAGAAGGAGAATACCAAGGAACAAGCTGTGATCCCACTTACAGATTCAGAGTATAAGCTGCTGCCCTTGCACTTTGCTGTGGACCCTGGAAAGGGCTGGGAGTGGGGCAAAGATGATAGTGACAATGTCCGATTGGCCAGTGTAATTCTGTCCCTAGAGGTCAAATTGCATCTGCTGCATAGCTACATGAATGTGAAGTGGATTCCACTGTCCTCTTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSTKTKQS
NRAKCLAKRKIAQMTEEEQFALALKMSEQEAREVNSQEEEEEELLRKAIA
ESLNSCRPSPPKKKRKVNEHPLEMPICAFQLPDLTVYNEDFRSFIERDLI
EQSMLVALEQAGRLNWWVSVDPTSQRLLPLATTGDGNALLHAASLGMWGF
HDRDLMLRKALYALMEKGVEKEALKRRWRWQQTQQNKESGLVYTEDEWQK
EWNELIKLASSEPRMHLGTNGANCGGVESSEEPVYESLEEFHVFVLAHVL
RRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQ
AHFSALVSMEQKENTKEQAVIPLTDSEYKLLPLHFAVDPGKGWEWGKDDS
DNVRLASVILSLEVKLHLLHSYMNVKWIPLSS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49553
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: (with silent changes to kill internal BamHI sites)