cDNA Clone Fancd2 (mouse)

• Synonyms: 2410150O07Rik; AU015151; BB137857; FA-D2; FA4; FACD; FAD; FANCD
• Plasmid: pBabeD puro mouse fancd2 K559R
• Parent Plasmid: pBabeD puro
• Expressed: untagged mouse fancd2 K559R
• Genbank: NM_001033244.3 GI:282721082 with silent change t4203c to kill internal BamHI
• Tag: untagged
• Species: Mouse

Sequence of Insert

GGATCCATGATTTCCAAAAGACGTCGGCTAGATTCTGAGGATAAAGAAAACCTGACAGAAGATGCCTCCAAAACCATGCCCCTTTCCAAGCTGGCAAAGAAGTCTCACAATTCTCATGAAGTTGAAGAAAATGGCAGTGTCTTTGTAAAGCTTCTTAAGGCTTCAGGACTCACTCTTAAAACTGGAGAGAACCAAAATCAGCTAGGTGTGGATCAGGTAATCTTCCAAAGGAAGCTCTTTCAGGCCTTGAGGAAGCATCCTGCTTATCCCAAAGTAATAGAAGAGTTTGTTAATGGCCTGGAGTCCTACACTGAGGACAGTGAGAGTCTCAGGAACTGCCTGCTGTCTTGTGAGCGCCTGCAGGATGAGGAAGCCAGCATGGGCACATTTTACTCCAAGAGTCTGATCAAGCTACTTCTGGGGATTGACATTTTACAGCCTGCCATTATCAAAATGTTATTTGAAAAAGTGCCTCAGTTTCTTTTTGAAAGTGAGAACAGAGATGGAATCAACATGGCCAGACTCATTATCAATCAACTAAAATGGCTGGATAGAATTGTGGATGGCAAGGACCTCACGGCCCAGATGATGCAGTTGATCAGTGTTGCTCCCGTGAACTTACAGCATGACTTCATCACGAGCCTTCCTGAAATCCTAGGGGATTCCCAGCATGCTAATGTGGGGAAAGAGCTTGGCGAGCTGCTGGTGCAGAATACTTCCCTGACTGTTCCAATTTTGGATGTCTTTTCCAGTCTCCGACTTGACCCCAACTTCCTGTCCAAGATCCGCCAGTTGGTGATGGGCAAGCTGTCATCTGTCCGTCTAGAGGATTTCCCTGTGATTGTAAAGTTCCTTCTTCATTCTGTAACAGACACCACTTCCCTTGAGGTCATTGCCGAGCTTCGGGAGAACTTGAACGTCCAGCAGTTTATTTTGCCGTCACGAATTCAGGCTTCCCAAAGCAAATTGAAAAGTAAAGGACTAGCAAGCTCTTCAGGAAATCAAGAGAACAGTGATAAAGACTGTATTGTTCTTGTCTTTGATGTAATAAAGTCAGCCATTAGATATGAGAAAACCATTTCAGAGGCCTGGTTTAAGGCAATTGAACGCATTGAGTCCGCGGCTGAACATAAGGCTTTGGACGTGGTCATGCTGCTCATCATCTACAGCACCAGCACGCAGACCAAGAAGGGCGTGGAGAAGCTGCTGAGAAACAAGATTCAGTCAGACTGCATTCAAGAACAGCTGCTTGACAGTGCGTTCTCTACACATTACCTGGTTCTTAAGGATATTTGCCCATCTATTCTTTTGCTGGCTCAGACTTTGTTTCACTCTCAAGACCAGAGGATCATTTTGTTTGGCAGTCTTCTGTACAAATATGCTTTTAAGTTTTTTGATACTTACTGCCAGCAGGAAGTGGTTGGTGCCCTAGTCACCCATGTCTGCAGTGGGACTGAGGCTGAAGTCGACACTGCACTGGATGTCCTCCTGGAGCTGATTGTGCTAAACGCCTCTGCTATGAGGCTCAATGCTGCTTTTGTTAAGGGCATCTTAGATTATTTGGAAAATATGTCCCCTCAGCAAATACGAAAAATCTTCTGTATTCTCAGCACTCTTGCATTTAGCCAACAGCCCGGAACCAGCAACCATATCCAGGACGACATGCACCTGGTGATCCGGAGGCAGCTCTCTAGCACTGTGTTCAAGTACAAGCTCATTGGGATCATTGGTGCAGTCACCATGGCCGGCATCATGGCGGAAGACAGAAGTGTACCATCTAACTCATCCCAGAGGAGCGCCAATGTGAGCAGTGAGCAGCGCACACAGGTGACTTCTTTGCTACAACTAGTTCATTCTTGCACTGAGCACTCTCCTTGGGCCTCTTCTCTGTATTATGATGAATTTGCCAACCTGATCCAAGAAAGGAAGTTGGCTCCAAAAACCTTGGAGTGGGTTGGGCAGACCATCTTCAATGATTTCCAAGATGCCTTTGTGGTAGACTTCTGTGCTGCTCCAGAGGGTGACTTTCCATTTCCTGTGAAAGCGCTCTATGGACTGGAAGAGTACAGCACTCAAGACGGCATTGTCATCAACCTCCTGCCGCTGTTCTATCAGGAATGTGCAAAAGATGCCAGTCGAGCGACATCACAAGAATCGAGCCAGAGATCAATGTCTTCTTTGTGCCTGGCTTCCCATTTCCGGCTGCTGAGACTTTGCGTGGCAAGACAACATGATGGAAACTTGGATGAGATCGATGGTCTCTTAGATTGTCCCCTGTTCCTCCCTGACCTGGAACCTGGAGAGAAACTGGAGTCCATGTCTGCTAAAGACCGTTCGCTTATGTGTTCGCTCACATTCCTAACTTTCAACTGGTTCCGAGAGGTTGTGAATGCCTTCTGCCAACAAACATCTCCTGAGATGAAGGGCAAGGTTCTTAGTCGGCTAAAGGACCTTGTAGAACTTCAGGGAATCCTAGAGAAGTACTTGGCAGTCATCCCAGACTATGTTCCGCCTTTCGCAAGCGTTGACTTGGACACTTTAGATATGATGCCTAGGAGCAGTTCTGCTGTTGCAGCAAAAAACAGAAACAAGGGAAAGACGGGGGGAAAGAAACAAAAAGCTGATAGCAACAAAGCATCCTGTTCGGACACACTTCTAACAGAAGACACTTCAGAGTGTGACATGGCGCCATCTGGGAGAAGCCACGTAGACAAGGAGTCCACAGGGAAGGAAGGAAAGACGTTTGTGTCACTGCAGAATTACCGCGCTTTTTTCCGAGAGCTGGACATTGAGGTCTTCTCTATTCTACATTCTGGACTTGTGACCAAGTTCATCTTAGACACTGAAATGCACACTGAAGCTACAGAGGTCGTACAGCTGGGGCCTGCTGAGCTGCTCTTCTTGCTGGAAGATCTTTCCCAGAAGCTAGAGAATATGCTGACTGCTCCTTTTGCCAAGAGAATCTGCTGCTTTAAGAATAAAGGAAGGCAGAATATTGGCTTCTCACATCTTCATCAGAGATCTGTCCAGGACATTGTGCACTGTGTGGTTCAGCTGCTAACCCCGATGTGTAACCATCTGGAGAACATTCACAACTTCTTTCAGTGCTTAGGTGCTGAGCATCTCAGTGCAGATGACAAGGCGAGAGCGACAGCTCAGGAGCAGCACACCATGGCCTGCTGCTACCAGAAGCTGCTGCAGGTCTTGCACGCGCTCTTTGCGTGGAAGGGATTTACTCACCAATCAAAGCACCGCCTCCTGCACTCAGCCCTTGAGGTCCTCTCGAACCGACTAAAGCAGATGGAACAGGACCAGCCCTTGGAGGAACTGGTCAGCCAGAGCTTCAGTTACTTGCAGAACTTCCACCATAGTGTTCCCAGTTTCCAGTGTGGTCTCTACCTTCTCAGACTTCTGATGGCCCTTCTGGAGAAGTCTGCAGTACCTAACCAGAAGAAAGAAAAACTTGCCTCTCTGGCCAAACAGCTGCTTTGCCGAGCATGGCCTCATGGGGAAAAAGAGAAGAACCCCACTTTTAATGACCACCTGCATGATGTGCTTTACATCTACTTGGAGCACACAGACAATGTTCTGAAGGCCATAGAGGAGATCACTGGTGTTGGTGTCCCAGAACTGGTCAGTGCTCCGAAAGACGCCGCCTCCTCTACATTCCCTACGTTGACCAGGCACACCTTTGTCATATTCTTCCGTGTGATGATGGCTGAACTCGAGAAGACGGTGAAGGGTCTTCAGGCTGGCACAGCAGCAGATTCGCAGCAGGTTCACGAAGAGAAGCTCCTCTATTGGAACATGGCTGTCCGAGATTTCAGCATCCTTCTCAATCTGATGAAAGTATTTGACAGTTATCCTGTTCTGCATGTGTGTTTAAAGTATGGCCGTCGCTTTGTGGAGGCATTTCTGAAGCAATGTATGCCACTCCTCGACTTCAGCTTTAGAAAGCATCGGGAAGATGTTCTGAGCTTGCTGCAAACCCTTCAGTTGAACACGAGGCTACTTCATCACCTTTGTGGACACTCCAAGATTCGCCAGGACACAAGACTCACCAAGCATGTGCCTTTACTCAAAAAGTCACTGGAACTGTTAGTTTGCAGAGTCAAAGCCATGCTTGTCCTCAACAACTGTAGAGAGGCTTTCTGGTTGGGTACTCTCAAAAACCGAGACTTACAGGGTGAAGAAATTATTTCCCAGGACCCCTCTTCCTCAGAGAGCAATGCAGAGGACAGTGAGGATGGCGTGACATCTCACGTCTCCAGGAACAGAGCAACAGAGGATGGGGAAGATGAAGCAAGTGATGAACAGAAGGACCAGGACAGTGATGAAAGTGACGACAGCTCCAGTTAGCGGCCGC

Amino Acid Sequence

MISKRRRLDSEDKENLTEDASKTMPLSKLAKKSHNSHEVEENGSVFVKLL
KASGLTLKTGENQNQLGVDQVIFQRKLFQALRKHPAYPKVIEEFVNGLES
YTEDSESLRNCLLSCERLQDEEASMGTFYSKSLIKLLLGIDILQPAIIKM
LFEKVPQFLFESENRDGINMARLIINQLKWLDRIVDGKDLTAQMMQLISV
APVNLQHDFITSLPEILGDSQHANVGKELGELLVQNTSLTVPILDVFSSL
RLDPNFLSKIRQLVMGKLSSVRLEDFPVIVKFLLHSVTDTTSLEVIAELR
ENLNVQQFILPSRIQASQSKLKSKGLASSSGNQENSDKDCIVLVFDVIKS
AIRYEKTISEAWFKAIERIESAAEHKALDVVMLLIIYSTSTQTKKGVEKL
LRNKIQSDCIQEQLLDSAFSTHYLVLKDICPSILLLAQTLFHSQDQRIIL
FGSLLYKYAFKFFDTYCQQEVVGALVTHVCSGTEAEVDTALDVLLELIVL
NASAMRLNAAFVKGILDYLENMSPQQIRKIFCILSTLAFSQQPGTSNHIQ
DDMHLVIRRQLSSTVFKYKLIGIIGAVTMAGIMAEDRSVPSNSSQRSANV
SSEQRTQVTSLLQLVHSCTEHSPWASSLYYDEFANLIQERKLAPKTLEWV
GQTIFNDFQDAFVVDFCAAPEGDFPFPVKALYGLEEYSTQDGIVINLLPL
FYQECAKDASRATSQESSQRSMSSLCLASHFRLLRLCVARQHDGNLDEID
GLLDCPLFLPDLEPGEKLESMSAKDRSLMCSLTFLTFNWFREVVNAFCQQ
TSPEMKGKVLSRLKDLVELQGILEKYLAVIPDYVPPFASVDLDTLDMMPR
SSSAVAAKNRNKGKTGGKKQKADSNKASCSDTLLTEDTSECDMAPSGRSH
VDKESTGKEGKTFVSLQNYRAFFRELDIEVFSILHSGLVTKFILDTEMHT
EATEVVQLGPAELLFLLEDLSQKLENMLTAPFAKRICCFKNKGRQNIGFS
HLHQRSVQDIVHCVVQLLTPMCNHLENIHNFFQCLGAEHLSADDKARATA
QEQHTMACCYQKLLQVLHALFAWKGFTHQSKHRLLHSALEVLSNRLKQME
QDQPLEELVSQSFSYLQNFHHSVPSFQCGLYLLRLLMALLEKSAVPNQKK
EKLASLAKQLLCRAWPHGEKEKNPTFNDHLHDVLYIYLEHTDNVLKAIEE
ITGVGVPELVSAPKDAASSTFPTLTRHTFVIFFRVMMAELEKTVKGLQAG
TAADSQQVHEEKLLYWNMAVRDFSILLNLMKVFDSYPVLHVCLKYGRRFV
EAFLKQCMPLLDFSFRKHREDVLSLLQTLQLNTRLLHHLCGHSKIRQDTR
LTKHVPLLKKSLELLVCRVKAMLVLNNCREAFWLGTLKNRDLQGEEIISQ
DPSSSESNAEDSEDGVTSHVSRNRATEDGEDEASDEQKDQDSDESDDSSS
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49554
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee