cDNA Clone DWNK

• Plasmid: pGEX6P1 WNK 214-959 (drosophila)
• Parent Plasmid: pGEX6P1
• Expressed: GST DWNK 214-959
• Genbank: AGB94864
• Tag: GST
• Species: Drosophila Melanogaster

Sequence of Insert

GGATCCATGGAAGCACGTAAAAGCAGCAGCGATACCGTTATTGAACCGCTGATTAAACAGCAGGCACCGCTGCATAGCAGCAAAACCACCCCGACCACCC
TGACCGCAAATTTTGTTCAGAATATTCGTTTCGTGCGCAAAAATGTTGAACAGAGCGGTCGTAATACCAATCCGCTGCAGTTTGTTGAACTGGAAACCGA
ATTTCCGCGTGATTATGATGATAACATTGAAATGCTGAGCCGTGAAGCAGAACATCTGGAAGAACAGTTTCGTACCCCGACCCGTAGCAATGCAACCGAT
GCAACCAGCCATCATGTTGCAGGTATTATTGATAACATCATTACCGAAGCAAGCCGTAGCCTGACCATTGAAAAAACCGAAGATGATGTTCCGCTGAAAA
GCAGTACCAAACATAGCAGCGGTGTTAAACGTGTTGGTTTTCAGGTTGAGGATAAAGATGATACCGAAGTGCAGAGCGAAAAACAGGCAAAAAGCTTTGA
TGATATTACCAAAAAAGCCGAAAGCAGCGAAGCCAGCGCAGAAGAAGCAGCAGTTACCGGTAGCAGCACCGATGCGAGCGCAAGTCCGCTGCCGAGCACC
AGCCTGGTTAGCACCACCAGTAGCGCAACCAGCATTACCAAATCAAAATCCGATGAGGATGATGATCCGGTTGCAATGAGCCCGTGTGGTCGCTTTTTCA
AATATGATAAAGAAGTTGGTCGCGGTAGCTTCAAAACCGTTTATCGTGGTCTGGATACCCTGACAGGTGTTCCGGTTGCCTGGTGTGAACTGCTGGATAA
ACAGGTGAAAAAAAGCGAACGTACCCGTTTTCGTGAAGAGGCAGATATGCTGAAAAAACTGCAACATCCGAATATCGTGCGCTTCTATACCTATTGGGAA
TTTCCGATTGGTCGCAAAAAAAACATTGTTCTGGTTACCGAACTGATGCTGAGCGGCACCCTGAAAAGCTATCTGAAACGCTTCAAAAAAATCCACCCGA
AAGTTCTGAAAAGTTGGTGTCGTCAGATTCTGAAAGGTCTGAATTTTCTGCATACCCGTCAGTTTCCGATTATTCATCGTGATCTGAAATGCGACAACAT
TTTCATTACCGGTACAACCGGTAGCGTGAAAATTGGTGATCTGGGTCTGGCAACACTGAAAAATCGTAGCCATGCAAAAAGCGTTATTGGCACACCGGAA
TTTATGGCACCGGAAATGTATGAAGAACACTATGATGAAAGCGTGGATGTGTATGCATTTGGTATGTGCATGCTGGAAATGGCGATTAGCGAATATCCGT
ATAGCGAATGTAAAGGTCCGGCACAGATTTACAAAAAAGTGATTAGCGGTATTAAACCGGCAGCACTGGCAAAAGTTGAAGATCCGAATGTTCGCGATAT
TATCGAACGTTGCATCGAACTGAAAAAAGAAGATCGTCCGAGCTGTAATGAGCTGCTGGAAAGTGAATTTTTTGATGAGGATATTGGCATTCGTGTTGAA
CCGACCGCAAGCGAACAGTTCCTGAGCGATCCGAGCATTAGCATTATTGAATTTCGTCTGCGTTTCATGGACCCGAAAAAACGTAGCAGCCGTCATAAAG
AAAATGAAGCCATTCAGTTTGAGTACAACATCCGCCATGATGAATATGAGCAGATTGCACAAGAAATGATGAAAGAAAACATCATCAGCGAGGATGATAG
CCGTGCAGTTGCACGTCTGCTGAAAGTTCAGGTTGTTAGCCTGCTGAAAGAACGTGCACAGCGTCAGACACAGATTAAACTGCAGAATGAAAAAAGTCGC
CTGGAAAAACTGGCACTGCAGAAACAGCGTGAAAGCCTGCCGACCAATGTTGATGAAGATGAAGAAGAAGAGGAAGAATCTGAGGACGAAGAGGATGGTG
TTAAATGGAATCAGCGTCTGCAGCTGCGTTATGATCTGCTGAATACCGATAGTGAAACCAGTCTGGCACTGAGCACCAATAGCGTTGAACCGCAGCAGCT
GAGCACCCGTAGTAATACCAGCATTCCGAATAGCGGCATTCAGCAGCCGGTTCAGGTTCCGGGTCAGGTGCCGGTTAGCCAGCTGATTAGCGTTCAGCCG
CAGGCAATTCCGAGCCCTGCCATTCCGATGCAGCAGAAACCGACCGTTCATTATATTCAGCCTCCGCAGCTGGCAAGCTATCAGAATAGCAATGCGAGCA
TGCAAGAGATGACCAATAATCAGGTGATTAGCCCGACCGGTAGTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEARKSSSDTVIEPLIKQQAPLHSSKTTPTTLTANFVQNIRFVRKNVEQSGRNTNPLQFVELETEFPRD
YDDNIEMLSREAEHLEEQFRTPTRSNATDATSHHVAGIIDNIITEASRSLTIEKTEDDVPLKSSTKHSSGVKRVGFQVEDKDDTEVQSEKQAKSFDDITK
KAESSEASAEEAAVTGSSTDASASPLPSTSLVSTTSSATSITKSKSDEDDDPVAMSPCGRFFKYDKEVGRGSFKTVYRGLDTLTGVPVAWCELLDKQVKK
SERTRFREEADMLKKLQHPNIVRFYTYWEFPIGRKKNIVLVTELMLSGTLKSYLKRFKKIHPKVLKSWCRQILKGLNFLHTRQFPIIHRDLKCDNIFITG
TTGSVKIGDLGLATLKNRSHAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMAISEYPYSECKGPAQIYKKVISGIKPAALAKVEDPNVRDIIERC
IELKKEDRPSCNELLESEFFDEDIGIRVEPTASEQFLSDPSISIIEFRLRFMDPKKRSSRHKENEAIQFEYNIRHDEYEQIAQEMMKENIISEDDSRAVA
RLLKVQVVSLLKERAQRQTQIKLQNEKSRLEKLALQKQRESLPTNVDEDEEEEEESEDEEDGVKWNQRLQLRYDLLNTDSETSLALSTNSVEPQQLSTRS
NTSIPNSGIQQPVQVPGQVPVSQLISVQPQAIPSPAIPMQQKPTVHYIQPPQLASYQNSNASMQEMTNNQVISPTGS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46624
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee