cDNA Clone Ufd1 (yeast)

• Plasmid: pET15 6His 3C yeast Ufd1
• Parent Plasmid: pET15b 6His 3C R1
• Expressed: 6His 3C yeast Ufd1
• Genbank: NM_001181177.1 GI:296145085 with silent change to kill internal BamHI
• Tag: 6His
• Species: Yeast

Sequence of Insert

GGATCCATGTTTTCTGGCTTTAGTTCTTTTGGCGGCGGAAATGGTTTTGTAAATATGCCTCAAACGTTTGAGGAGTTCTTTAGATGCTACCCTATAGCTATGATGAATGATAGAATACGGAAGGATGACGCAAACTTCGGCGGGAAAATATTCCTGCCACCAAGTGCTCTAAGCAAACTATCCATGTTGAACATAAGATACCCCATGCTTTTTAAACTAACTGCCAATGAAACTGGACGTGTGACACATGGAGGTGTTTTAGAATTTATTGCTGAAGAGGGCAGAGTTTACTTACCACAATGGATGATGGAAACTTTGGGTATACAGCCAGGATCTCTACTGCAAATTTCATCTACTGATGTACCTCTGGGCCAGTTTGTGAAGTTAGAACCTCAGTCTGTAGATTTCTTGGATATTTCCGACCCCAAAGCAGTTTTGGAAAACGTACTGAGGAATTTCTCGACTTTGACCGTGGACGATGTTATTGAGATTAGTTACAATGGTAAAACTTTCAAAATTAAGATCTTAGAAGTCAAACCAGAATCATCATCGAAGAGTATATGTGTCATTGAAACAGATTTAGTAACAGATTTTGCTCCACCTGTAGGATATGTGGAACCGGATTACAAAGCGCTGAAAGCTCAGCAAGATAAAGAAAAAAAGAATTCTTTTGGTAAGGGCCAAGTTTTGGACCCCTCGGTGCTTGGCCAGGGATCGATGTCCACAAGAATCGACTATGCGGGTATCGCTAACAGTAGCAGAAACAAACTATCTAAATTTGTAGGTCAGGGACAAAACATATCTGGTAAAGCTCCGAAAGCGGAGCCCAAGCAAGATATAAAAGATATGAAGATAACGTTTGATGGCGAGCCCGCCAAGCTAGATCTGCCTGAAGGCCAATTATTCTTTGGTTTCCCAATGGTCCTACCGAAAGAAGACGAAGAAAGTGCCGCTGGTAGCAAATCCAGTGAACAAAATTTCCAAGGCCAAGGGATCTCACTAAGGAAGAGCAATAAGAGAAAAACGAAAAGTGACCATGATTCGTCCAAGTCCAAGGCTCCCAAAAGCCCGGAAGTAATCGAAATTGACTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMFSGFSSFGGGNGFVNMPQTFEEFFRC
YPIAMMNDRIRKDDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTANETG
RVTHGGVLEFIAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKL
EPQSVDFLDISDPKAVLENVLRNFSTLTVDDVIEISYNGKTFKIKILEVK
PESSSKSICVIETDLVTDFAPPVGYVEPDYKALKAQQDKEKKNSFGKGQV
LDPSVLGQGSMSTRIDYAGIANSSRNKLSKFVGQGQNISGKAPKAEPKQD
IKDMKITFDGEPAKLDLPEGQLFFGFPMVLPKEDEESAAGSKSSEQNFQG
QGISLRKSNKRKTKSDHDSSKSKAPKSPEVIEID*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU49707
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee