cDNA Clone POH1

• Synonyms: PAD1; PSMD14; RPN11
• Plasmid: pcDNA5D FRT/TO GFP POH1
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP-POH1
• Genbank: NM_005805.5 GI:375268695
• Tag: GFP
• Species: Human

Sequence of Insert

AGATCTATGGACAGACTTCTTAGACTTGGAGGAGGTATGCCTGGACTGGGCCAGGGGCCACCTACAGATGCTCCTGCAGTGGACACAGCAGAACAAGTCTATATCTCTTCCCTGGCACTGTTAAAAATGTTAAAACATGGCCGTGCTGGAGTTCCAATGGAAGTTATGGGTTTGATGCTTGGAGAATTTGTTGATGATTATACCGTCAGAGTGATTGATGTGTTTGCTATGCCACAGTCAGGAACAGGTGTCAGTGTGGAGGCAGTTGATCCAGTGTTCCAAGCTAAAATGTTGGATATGTTGAAGCAGACAGGAAGGCCGGAGATGGTTGTTGGTTGGTATCACAGTCACCCTGGCTTTGGTTGTTGGCTTTCTGGTGTGGATATCAACACTCAGCAGAGCTTTGAAGCCTTGTCGGAGAGAGCTGTGGCAGTGGTTGTGGATCCCATTCAGAGTGTAAAAGGAAAGGTTGTTATTGATGCCTTCAGATTGATCAATGCTAATATGATGGTCTTAGGACATGAACCAAGACAAACAACTTCGAATCTGGGTCACTTAAACAAGCCATCTATCCAGGCATTAATTCATGGACTAAACAGACATTATTACTCCATTACTATTAACTATCGGAAAAATGAACTGGAACAGAAGATGTTGCTAAATTTGCATAAGAAGAGTTGGATGGAAGGTTTGACACTTCAGGACTACAGTGAACATTGTAAACACAATGAATCAGTGGTAAAAGAGATGTTGGAATTAGCCAAGAATTACAATAAGGCTGTAGAAGAAGAAGATAAGATGACACCTGAACAGCTGGCAATAAAGAATGTTGGCAAGCAGGACCCCAAACGTCATTTGGAGGAACATGTGGATGTACTTATGACCTCAAATATTGTCCAGTGTTTAGCAGCTATGTTGGATACTGTCGTATTTAAATAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMDRLLR
LGGGMPGLGQGPPTDAPAVDTAEQVYISSLALLKMLKHGRAGVPMEVMGL
MLGEFVDDYTVRVIDVFAMPQSGTGVSVEAVDPVFQAKMLDMLKQTGRPE
MVVGWYHSHPGFGCWLSGVDINTQQSFEALSERAVAVVVDPIQSVKGKVV
IDAFRLINANMMVLGHEPRQTTSNLGHLNKPSIQALIHGLNRHYYSITIN
YRKNELEQKMLLNLHKKSWMEGLTLQDYSEHCKHNESVVKEMLELAKNYN
KAVEEEDKMTPEQLAIKNVGKQDPKRHLEEHVDVLMTSNIVQCLAAMLDT
VVFK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49728
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. This clone is slow-growing. May need 48 hours on plate and in liquid culture.