cDNA Clone DCUN1D2

• Synonyms: C13orf17
• Plasmid: pU6 DCUN1D2 gRNA B
• Parent Plasmid: pU6 gRNA BamHI
• Expressed: DCUN1D2 gRNA B
• Genbank: ENSG00000150401
• Species: Human

Sequence of Insert

gGCTGGAGCGGCTGTACGGC(AGG)

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48393
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Expresses DCUN1D2 guide RNA B, 19mer target sequence shown as sequence of insert with remaining 3 bases of PAM site in brackets (extra g added to optimise length). Targets coding region in exon 2, PAM site is situated on the sense strand. To be used in conjunction with DCUN1D2 guide RNA A, a ssDNA KO donor and Cas9 nickase.